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Detection pad for heavy metal mercury in chemical wastewater and preparation method thereof

A technology for chemical sewage and heavy metals, which is applied in the direction of measuring devices, instruments, and material analysis through optical means, can solve problems such as endangering human health, damage, and excessive heavy metal pollution, and achieve the effect of SERS detection

Inactive Publication Date: 2019-07-02
湖南泰华科技检测有限公司
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Problems solved by technology

[0003] The discharge of industrial mercury waste water and other problems not only cause serious mercury pollution in the environment, but also cause excessive heavy metal pollution in agricultural products, which seriously affects the quality of agricultural products in my country and international competitiveness, and also seriously endangers human health. The main manifestations of mercury's harm to the human body are In causing kidney damage (in the form of inorganic mercury), central nervous system disorders, mental retardation, and even death (in the form of organic mercury), mercury pollution and other heavy metal pollution often cause compound pollution of two or more heavy metal elements, and It has the characteristics of universality and complexity, which brings greater pressure to the ecological environment. Therefore, the detection of heavy metal mercury pollution in the environment has attracted more and more attention.

Method used

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preparation example Construction

[0022] A detection pad and preparation method of heavy metal mercury in chemical sewage shown in the present invention include a detection pad, which is a detection pad nitrocellulose membrane, which is coated with Fe3O4 / SiO2 magnetic particles, and the preparation of the magnetic particles includes Follow the steps below:

[0023] 1) Fe3O4 / SiO2 nanoparticles were prepared by hydrolysis of tetraethyl silicate (TEOS) and high-temperature separation, and 50mg of Fe3O4 / SiO2 magnetic particles were dispersed in 100mL of 2% polydiallyldimethylammonium chloride (PDDA) In the solution, react for 20 minutes under mechanical stirring, and wash the generated precipitate with deionized water to obtain PDDA-modified Fe3O4 / SiO2 magnetic particles.

[0024] 2) Preparation of Fe3O4 / Au magnetic particles: Disperse 0.05g of PDDA-modified Fe3O4 / SiO2 magnetic particles obtained in step (1) in 200mL of Au sol, put them in a shaker, react at 30°C for 20min, and use deionized Wash with water to ob...

Embodiment 1

[0031]Preparation of Fe3O4 / Au nanoparticles: Mix 0.65g FeCl3 6H2O, 0.118g sodium citrate, 1.2g sodium acetate and 20mL ethylene glycol and stir magnetically for 0.5h, put them in a polytetrafluoro reactor at 200°C for 10h, heat After finishing, cool to room temperature, wash with ethanol 3 times, deionized water 3 times, and dry in a vacuum oven at 50°C for 2 hours to obtain Fe3O4 magnetic particles; preparation of Fe3O4 / SiO2 magnetic particles: take 0.1g Fe3O4 and disperse in 20mL nitric acid solution ( 0.1mol / L), ultrasonic for 10min, washed 6 times with deionized water, dispersed in the mixed solution (20mL ethanol + 4mL deionized water + 1mL ammonia water), ultrasonic for 10min, poured into a three-neck round bottom flask, under mechanical stirring Add 0.8mL TEOS dropwise to it continuously, and after the reaction is carried out for 3h, the resulting precipitate is dried in vacuum at 60°C for 1h to obtain Fe3O4 / SiO2 nanoparticles; 0.1 mgFe3O4 / SiO2 magnetic particles are dis...

Embodiment 2

[0033] Preparation of nucleic acid aptamer-modified SERS substrate: disperse 0.015g FA magnetic particles in 50mL of PBS solution (0.4g NaCl+0.01g KCl+0.072g Na2HPO4+0.012g KH2PO4), put it in a shaker and react at 30°C for 60min , wash the resultant with PBS and dissolve it in 15mL PBS, add 118μL DNA solution (5′-Cy5-TTGTTTGTCCCCTCTTTCTTA-(CH2)3-SH-3′) with a concentration of 100μM / L, and place it on a shaker at 30°C After reacting for 3 hours, add 1 mL of an aqueous solution containing 17.5 mg of NaCl, incubate for 12 hours, wash the resultant three times with PBS, and dissolve it in 0.5 mL of PBS to prepare aptamer-modified FA-DNA magnetic particles.

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Abstract

The invention discloses a detection pad for heavy metal mercury in chemical wastewater and a preparation method thereof. The detection pad for heavy metal mercury in chemical wastewater comprises a detection pad, the detection pad is a cellulose nitrate membrane, and the membrane is coated with Fe3O4 / SiO2 magnetic particles. The magnetic particle preparation comprises the following steps: Fe3O4 / SiO2 nanoparticles are prepared through a tetraethoxy-silicone (TEOS) hydrolysis method and a high temperature separation method, 50 mg of Fe3O4 / SiO2 nanoparticles are dispersed in 100 mL of a 2% poly(diallyldimethylammonium chloride) (PDDA) solution, the reaction is carried out for 20 min under mechanical stirring, and the generated precipitate is washed with deionized water to obtain PDDA-modifiedFe3O4 / SiO2 magnetic particles. The invention prepares a Fe3O4 / Au core-shell magnetic material, carrying is carried out through a carrier, aptamer modification is carried out on the surface of a substrate, T-Hg-T effects between Hg ions and the aptamer and the magnetic properties of the substrate are used to realize enrichment and separation of mercury ions in an actual water sample, and SERS detection on the mercury ions is also realized.

Description

technical field [0001] The invention relates to the technical field of heavy metal mercury detection, in particular to a detection pad for heavy metal mercury in chemical sewage and a preparation method. Background technique [0002] my country is one of the main mercury-producing countries in the world, and also one of the countries with the most serious mercury pollution. The heavy metal mercury is an important metal element that exists in nature. Due to its many excellent properties, mercury and its compounds are widely used in industrial and agricultural production. However, mercury is a metal pollutant with strong toxicity to human body and higher organisms. As early as the 1950s, the Minamata disease in Kumamoto, Japan After the outbreak, the pollution of mercury has attracted people's attention. In recent years, with the continuous acceleration of urbanization and the rapid improvement of the level of industrialization, the pollution of mercury and its compounds cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N21/01
CPCG01N21/01G01N21/658
Inventor 陈龙何月云沈亮
Owner 湖南泰华科技检测有限公司
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