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Extraction separation method for total lactones of ginkgo

A technology of total ginkgo lactone and separation method is applied in the field of extraction and separation of total ginkgo lactone, can solve the problems of long production cycle, complicated operation, unfavorable industrialized production, etc., and achieves high quality, low ginkgo phenolic acid content and cost saving Effect

Pending Publication Date: 2019-07-09
CHENGDU BAIYU PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The patent application publication number CN1977868A discloses a method for extracting total ginkgo lactones, which comprises the steps of ethanol water extraction followed by macroporous resin separation, polyamide resin, elution, ethyl acetate extraction, and drying. The method not only requires Through two kinds of resin columns, the operation is more complicated, and the production cycle is long, which is not conducive to industrial production

Method used

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  • Extraction separation method for total lactones of ginkgo
  • Extraction separation method for total lactones of ginkgo
  • Extraction separation method for total lactones of ginkgo

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Example 1 The extraction and separation method of the present invention

[0047] Take 10kg of crushed Ginkgo biloba leaves in a 100L reactor, add 50L of 70% ethanol aqueous solution and heat to 60-80°C for 3 times, combine the extracts, filter the filtrate and concentrate at 50-60°C under reduced pressure to recover ethanol and concentrate 物A.

[0048] Concentrate A was applied to a 5L polyamide resin column and eluted with 3BV of purified water. The eluate was collected and concentrated under reduced pressure at 50-60°C to obtain Concentrate B.

[0049] Concentrate B was first extracted twice with n-heptane, each extraction volume was 5L; then it was extracted three times with ethyl acetate, each volume was 4L, and the combined organic phases were concentrated under reduced pressure to recover ethyl acetate to obtain concentrate C.

[0050] Concentrate C was heated and dissolved in 2L of 95% ethanol, 20g activated carbon was added, heated to 50-60℃, decolorized for 50min, filte...

Embodiment 2

[0052] Example 2 The extraction and separation method of the present invention

[0053] Take 10kg of crushed Ginkgo biloba leaves in a 100L reactor, add 50L of 70% ethanol aqueous solution and heat to 60-80°C for 3 times, combine the extracts, filter the filtrate and concentrate at 50-60°C under reduced pressure to recover ethanol and concentrate 物A.

[0054] After filtration, the filtrate was applied to a 10L polyamide resin column and eluted with 3BV of purified water. The eluent was collected and concentrated under reduced pressure at 50-60°C to obtain concentrate B.

[0055] Concentrate B was first extracted with petroleum ether twice with a volume of 5L for each extraction; then extracted three times with ethyl acetate with a volume of 4L each. The combined organic phases were concentrated under reduced pressure to recover ethyl acetate to obtain a concentrate C.

[0056] After the concentrate C was heated and dissolved in 2L of 95% ethanol, 20g activated carbon was added, the tem...

Embodiment 3

[0058] Embodiment 3 The extraction and separation method of the present invention

[0059] Take 10 kg of crushed ginkgo leaves in a 100L reactor, add 50L of 70% ethanol aqueous solution and heat to 70°C for two extractions, combine the extracts, concentrate under reduced pressure at 50-60°C to recover ethanol to obtain concentrate A.

[0060] After filtration, the filtrate was applied to a 10L polyamide resin column and eluted with 3BV of purified water. The eluent was collected and concentrated under reduced pressure at 50-60°C to obtain concentrate B.

[0061] Concentrate B was first extracted twice with n-hexane, each extraction volume was 5L; then it was extracted three times with ethyl acetate, each volume was 4L, the combined organic phases were concentrated under reduced pressure to recover ethyl acetate to obtain concentrate C.

[0062] After the concentrate C was heated and dissolved in 2L of 95% ethanol, 20g activated carbon was added, the temperature was raised to 50-60℃, th...

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Abstract

The invention provides an extraction separation method for total lactones of ginkgo. The method comprises the following steps: (1) extracting; (2) passing through an amide resin column; (3) extracting; and (4) crystallizing. The invention further provides the total lactones, acquired according to the method, of ginkgo. After extraction and separation according to the method, total content of the total lactones of ginkgo reaches 90% or more, and a yield is high, the content of a harmful ingredient phenolic acid is low extremely, operation is simple and convenient, a process route is rational, and the method is suitable for industrialized mass production.

Description

Technical field [0001] The invention belongs to the technical field of Chinese medicine extraction, and specifically relates to a method for extracting and separating ginkgo total lactones. Background technique [0002] Ginkgo biloba L. is one of the oldest rare tree species in the world. Ginkgo biloba has been used for medicine for more than 500 years. Ginkgo biloba total lactones are unique components in Ginkgo biloba leaves with special molecular structure and significant pharmacological activity. They belong to terpenoids, also known as terpene lactones, including diterpene lactones and sesquiterpene lactones. Modern studies have shown that Ginkgo biloba total lactone has a strong and specific inhibitory effect on platelet activating factor receptors, and also has a protective effect on single ischemic injury and central nervous system. It also has anti-allergic, anti-shock, and anti-organ transplant rejection. Therefore, total ginkgo lactones have high medicinal value and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16
CPCA61K36/16A61K2236/333A61K2236/51A61K2236/55
Inventor 李大雄柯鸿樊小波孙毅其他发明人请求不公开姓名
Owner CHENGDU BAIYU PHARMA CO LTD