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A kind of preparation method and application of ferroferric oxide/gold structure nanoparticles

A technology of ferric oxide and gold nanoparticles, which is used in the preparation of test samples, material excitation analysis, instruments, etc., and can solve the interference of background autofluorescence, high purity of the measured object, blood color and electrode pollution. and other problems, to achieve the effect of high sensitivity, strong specificity, and avoiding interference

Active Publication Date: 2022-02-01
SOUTH CHINA NORMAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

In 2018, Xie's research group used cobalt nitride nanoarrays as electrochemical sensors to detect hydrogen peroxide and glucose. 2 o 2 The detection limit of the method reached 1μM, and the detection limit of glucose reached 50nM, but the method was easily interfered by protein in blood, blood color and electrode contamination
However, this method is susceptible to interference from other colored components, and requires a high purity of the analyte; in 2018, Liu’s research group developed an iminocoumarin derivative based on an aromatic boronic acid ester as a sensing unit. A fluorescent probe for H 2 o 2 The detection limit reached 60nM
Although fluorometry can overcome the problems of the previous two methods, it is susceptible to interference from background autofluorescence

Method used

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  • A kind of preparation method and application of ferroferric oxide/gold structure nanoparticles
  • A kind of preparation method and application of ferroferric oxide/gold structure nanoparticles
  • A kind of preparation method and application of ferroferric oxide/gold structure nanoparticles

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Embodiment 1

[0055] A preparation method of ferric oxide / gold structure nanoparticles, comprising the following steps:

[0056] Step one, Fe 3 o 4 Preparation of nanoparticles: 65 mL of water was added to a 250 mL three-necked flask, and the water bath was heated to 90° C. in an oxygen-free environment with nitrogen. Weigh 1.280g of ferrous sulfate heptahydrate and dissolve it in 10mL of deionized water to obtain an aqueous solution of ferrous sulfate. In a three-neck flask, add 5mL of polyethyleneimine (relative molecular mass: 10000, concentration: 80mg / mL) (poly The final concentration of ethyleneimine is 4g / L), 10mL ferrous sulfate aqueous solution, 10mL potassium nitrate solution with a concentration of 2.0M, and 10mL sodium hydroxide solution with a concentration of 1.0M, and keep for 2h. The particles were settled with a magnet and washed 5 times with deionized water. Add 80mL deionized water to resuspend for later use, the Fe 3 o 4 Most of the diameters of nanoparticles are be...

Embodiment 2

[0065] Using the ferric oxide / gold nanoparticles prepared in Example 1 as a SERS probe, 3,3',5,5'-tetramethylbenzidine was used as a reporter molecule to detect H 2 o 2 Concentration involves the following steps:

[0066] Step 1. Preparation of a constant concentration gradient hydrogen peroxide solution: Take a certain amount of 30% hydrogen peroxide aqueous solution, and successively prepare 1 mL of a certain gradient concentration hydrogen peroxide aqueous solution.

[0067]Step 2. SERS detection of different concentrations of hydrogen peroxide solutions: For different concentrations of hydrogen peroxide solutions, add 100 μL of the above-mentioned functionalized probes, react for 15 minutes, magnetically pick up the probes, and drop them on analytical pure aluminum sheets for Raman testing. Among them, the wavelength of the Raman excitation light is 633nm, the power is 10%, the static mode is adopted, the exposure time is 1s, and the number of integrations is 2 times.

Embodiment 3

[0069] Using the ferric oxide / gold nanoparticles prepared in Example 1 as a SERS probe, using 3,3',5,5'-tetramethylbenzidine as a signal molecule, the detection of glucose concentration includes the following steps:

[0070] Step 1. Enzyme treatment of a certain concentration gradient glucose solution: Weigh a certain amount of glucose, and successively prepare 1 mL of a certain concentration gradient glucose aqueous solution. Add 20 μL of 20 mg / mL glucose oxidase GOx, and react in a water bath at 37.5 °C for 30 min;

[0071] Step 2, SERS detects glucose solutions of different concentrations: for a certain concentration gradient (1-10 -9 mol / L) glucose solution, add 100 μL of the above-mentioned functionalized probe, react for 15 min, pick up the probe magnetically, and add it dropwise onto an analytically pure aluminum sheet for Raman testing. Among them, the wavelength of the Raman excitation light is 633nm, the power is 10%, the static mode is adopted, the exposure time is...

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Abstract

The invention discloses a preparation method and application of ferric oxide / gold structure nanoparticles. The ferric oxide / gold structure nanoparticles of the present invention select 3,3',5,5'-tetramethylbenzidine (TMB) as the Raman reporter molecule, which is co-coated by gold-sulfur (Au-S) as the Raman reporter molecule. Valence bonds are attached to the gold surface to produce surface-enhanced Raman spectroscopy (SERS). Due to the catalytic effect of ferric oxide on the particles, hydrogen peroxide can rapidly oxidize 3,3',5,5'-tetramethylbenzidine in the detection, according to 3,3',5,5'-tetramethylbenzidine The change of SERS signal before and after the oxidation of benzidine molecule can realize the detection of H 2 O 2 Direct detection of concentration and indirect detection of glucose concentration. The advantages are high sensitivity and specificity, which can avoid the interference of other components in the sample during detection, and does not require pretreatment, and the operation steps are simpler. Not only that, but this method can be further upgraded to detect H in single cells 2 O 2 concentration.

Description

technical field [0001] The invention relates to the technical field of optical measuring instruments, and relates to a method for preparing ferric oxide / gold structure nanoparticles and an application thereof. Background technique [0002] The emergence of nanoscience and the development of nanotechnology have provided new opportunities for the design and construction of nanomaterial-based mimetic enzymes. Because mimic enzymes have the activity of natural enzymes, are easy to prepare and purify, have more tenacious resistance to temperature, acidity and inhibitors, etc., and have the advantages of high efficiency, strong specificity and low cost, therefore, mimic enzymes have become popular in recent years. The focus of research by scientists. Fe 3 o 4 The nanoparticles have similar enzymatic activity to natural horseradish peroxidase, making Fe-based 3 o 4 Nanoparticle-mimicking enzymes have become a hot area of ​​scientific research. [0003] Raman spectroscopy is t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N21/65
CPCG01N1/28G01N21/658
Inventor 钟丽云唐平张峰刘胜德吕晓旭
Owner SOUTH CHINA NORMAL UNIVERSITY
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