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Enzyme-polymer complex capable of decontaminating nerve agents and preparation method and applications thereof

A technology of nerve agents and complexes, applied in the field of enzyme-polymer complexes, can solve the problems of slow decontamination efficiency and personnel poisoning death, and achieve the effects of no toxic side effects, high safety, and high biocompatibility

Inactive Publication Date: 2019-07-16
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although biological enzyme decontaminants are mild, their decontamination efficiency is slow, and nerve agents are quick-killing toxins. If this type of material is used as a decontaminant for skin, etc., there will be a decontamination agent with slow enzymes. But people have been poisoned and died

Method used

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  • Enzyme-polymer complex capable of decontaminating nerve agents and preparation method and applications thereof
  • Enzyme-polymer complex capable of decontaminating nerve agents and preparation method and applications thereof
  • Enzyme-polymer complex capable of decontaminating nerve agents and preparation method and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The synthesis of embodiment 1 DFPase-pDMAEMA

[0075] 1. Isolation and purification of DFPase

[0076] (1) Weigh 650g of fresh pork liver, put it into a flask, cut it into small pieces, and add distilled water to mix.

[0077] (2) Add 100ml of cell lysate therein to fully rupture the pig liver tissue cells.

[0078] (3) Homogenize in batches with a homogenizer, and collect a total of 2000 ml of homogenate.

[0079] (4) Centrifuge the homogenate (10000r / min, 20min, 4°C), collect the supernatant to obtain a total of 1500ml of supernatant A (suspension).

[0080] (5) Heat-treat the supernatant A. The collected supernatant was placed in a water bath at 60°C for 10 min, and then quickly cooled in ice cubes. Then it was centrifuged (10000r / min, 10min, 4°C) to remove denatured protein impurities, and 1175ml of supernatant B solution (red, clear) was collected.

[0081] (6) Filter the supernatant B to remove impurities. Aspirate liquid B with a 50ml needle, and press filt...

Embodiment 2

[0098] The synthesis of embodiment 2.DFPase-F127

[0099] (1) Weigh 5g of F127 and put it into a two-necked pear-shaped bottle with a volume of 50ml. The system is sealed, vacuumized and then filled with nitrogen. The subsequent reactions in the pear-shaped bottle are carried out in a system filled with nitrogen. Mix 15ml of 1,4-dioxane and 110μl of triethylamine, then add 97mg of DMAP to dissolve completely, inject the solution into the pear-shaped bottle with a syringe, and keep stirring (1000r / min, 90min).

[0100] (2) Slowly drop the succinic anhydride solution (99mg succinic anhydride dissolved in 5ml 1,4-dioxane) into the pear-shaped bottle through the constant pressure dropping funnel, and continue to stir at room temperature (1000r / min , 41h).

[0101] (3) After the stirring was stopped, the solution was transferred to a 100ml pear-shaped bottle (the solution was almost oily and colorless). Use a rotary evaporator to remove excess 1,4-dioxane by suspension steaming (...

Embodiment 3D

[0108] Physical evaluation of embodiment 3DFPase-F127 and DFPase-pDMAEMA

[0109] (1) Morphological characterization. Take a small amount (about 150-200μl) of DFPase-F127 and DFPase-pDMAEMA aqueous solution (the concentration of DFPase is 0.2mg / ml) and drop it on the silicon wafer, and let it dry naturally at room temperature. After drying, a layer of nano-gold layer is sprayed to enhance the conductivity of the sample, and the sample is observed under a scanning electron microscope (SEM), see figure 1 , it can be seen that the synthesized DFPase-F127 and DFPase-pDMAEMA are nanoparticles with a size of about 200-300nm.

[0110] (2) Internal structure characterization. Take 50 μl of DFPase-F127 and DFPase-pDMAEMA aqueous solution (the concentration of DFPase is 0.2mg / ml) and drop it on the copper grid to air dry naturally. figure 2 , it can be seen that DFPase is a nearly round nanoparticle with a diameter of about 200-300nm; the density of the inner core of the nanopartic...

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Abstract

The present application provides an enzyme-polymer complex capable of decontaminating nerve agents, wherein the enzyme is selected from DFPase-like phosphoric triester hydrolases and the polymer is awater-soluble polymer. The invention also provides a preparation method of the complex and applications of the complex in decontaminating nerve agents. The complex can achieve extremely rapid decontamination on nerve agents and is mild and non-irritating to the skin.

Description

technical field [0001] The invention relates to the field of chemical defense, in particular to an enzyme-polymer complex, which can be used as a decontamination material for nerve agents. The complex can achieve extremely fast decontamination of nerve agents, has mild performance, and is non-irritating to skin, equipment, environment and the like. Background technique [0002] 1. Introduction to Nerve Agents [0003] Nerve agent is a kind of chemical warfare agent CWA (chemical warfare agent), which refers to the toxic chemical substance that destroys the normal conduction function of the nervous system. The injury routes of nerve agents include the skin, eyes, respiratory tract, and digestive tract, among which the skin has the largest surface area and is most likely to be contaminated with agents, causing poisoning or even death. [0004] Nerve agents are mainly divided into G-type agents and V-type agents. Their common features are: stability, that is, not easy to deco...

Claims

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Application Information

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IPC IPC(8): C12N9/16C08F220/34C08F222/38
CPCC08F220/34C12N9/16C12Y301/08C12Y301/08001C12Y301/08002C08F222/385
Inventor 王永安杨军王甲朋范丽雪孟凡荣李姚骆媛李万华全东琴隋昕高翔黄静宜
Owner ACADEMY OF MILITARY MEDICAL SCI
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