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Molecular imprinting electrochemical detection method for ketamine drugs

A molecular imprinting and detection method technology, applied in the field of molecular imprinting electrochemical detection of ketamine drugs, can solve the problems of inability to complete rapid on-site detection, affecting detection results, and long detection period, and achieves short detection time, high detection sensitivity, and detection. short cycle effect

Active Publication Date: 2019-07-26
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a molecularly imprinted electrochemical detection method for ketamine drugs, which can be directly used for the detection of human urine without the need for sample pretreatment, so as to solve the problem of interference in the detection of biological samples in existing detection methods As a result, a large amount of pretreatment work is required for the samples, the detection period is long, and the on-site rapid detection cannot be completed

Method used

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  • Molecular imprinting electrochemical detection method for ketamine drugs
  • Molecular imprinting electrochemical detection method for ketamine drugs
  • Molecular imprinting electrochemical detection method for ketamine drugs

Examples

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Embodiment 1

[0029] The method for molecularly imprinted electrochemical detection of ketamine drugs provided by the present invention includes the following steps:

[0030] 1. Preparation of high-purity graphene-loaded MOFs suspension: 1mg / ml graphene and 1mg / ml MOFs suspension were ultrasonically processed by an ultrasonic processor for 3 to 4 hours to make MOFs uniformly loaded on the graphene sheet.

[0031] 2. Preparation of graphene / MOFs modified electrode: Take 3μL of high-purity graphene-loaded MOFs suspension, drop it on the surface of the screen-printed electrode twice, and dry it naturally to obtain a graphene / MOFs modified electrode.

[0032] 3. Take 2μL of molecularly imprinted polymerization solution and apply dropwise to the graphene / MOFs modified electrode, place the UV lamp 22cm above the screen-printed electrode in a dark box, and initiate polymerization by UV for 180 minutes to obtain MIM / RGO / MOFs modification electrode.

[0033] Scanning electron microscopy experiments were per...

Embodiment 2

[0041] In order to further optimize the technical solution in Example 1, the amount of the high-purity graphene supported MOFs suspension in this example is 1.5-4 μL. When the dosage of high-purity graphene suspension is less than 3μL, the peak current will increase with the increase of dosage. After exceeding 3μL, the peak current will decrease with the increase of dosage. It is recommended to choose 3μL as the best dosage.

Embodiment 3

[0043] In order to further optimize the technical scheme in Example 1 and the polymerization conditions of the molecularly imprinted polymer membrane, in this example, ketamine was selected as the template molecule, MAA was the functional monomer and the cross-linking agent EGDMA, and the molar ratio of the three was 1:4 : 60; Methanol: 9:1 (volume ratio) of acetic acid as the eluent, eluted at a speed of 180 revolutions per second for 30 minutes, and then rinsed with deionized water several times; electrode to template molecules The adsorption time is 10min. At this time, the adsorption of ketamine on the imprinted membrane reached equilibrium. Because the incubation time continues to increase, the peak current basically no longer changes.

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Abstract

The invention relates to the technical field of drug detection, in particular to a molecular imprinting electrochemical detection method of ketamine drugs, which comprises the following steps: using amodified screen printing electrode as a working electrode, and determining the ketamine drugs in a buffer solution by using a cyclic voltammetry or a differential pulse voltammetry, wherein the screen printing electrode is respectively modified by a graphene RGO and MOFs composite nano material and a molecular imprinting polymer film and then washed, and ketamine is added into the buffer solution. The method has high sensitivity and good selectivity, can be directly used for detecting human urine without sample pretreatment, and solves the problems that in the existing detection method, the detection result is influenced by interferents in biological detection materials, a large amount of pretreatment work needs to be carried out on the sample, the detection period is long, and the fieldrapid detection cannot be completed.

Description

Technical field [0001] The invention relates to the technical field of drug detection, in particular to a molecular imprinting electrochemical detection method for ketamine drugs, which is used for detecting whether ketamine is contained in human urine. Background technique [0002] Ketamine, the main component of "K powder", chemical name: 2-(2-chlorophenyl)-2-(methylamino)cyclohexanone, molecular formula: C 13 H 16 CINO, a derivative of phencyclidine hydrochloride, is a dissociative anesthetic and a non-competitive antagonist of the N-methyl-D-aspartic acid (NMDA) subtype of excitatory amino acid receptors. Ketamine can regulate AMPA receptor throughput by antagonizing NMDA receptors and play an antidepressant effect. The sedative drug approved by the US Food and Drug Administration for the induction and maintenance of adult anesthesia can have a rapid and sustained antidepressant effect in depressed patients, including those with refractory depression. Clinically used for sur...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 白慧萍付开新王世雄张瑞林张艮林柳清菊
Owner YUNNAN UNIV
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