Preparation method and application of electrochemical immunosensor of PdCu nanowire functionalized porous graphene

A porous graphene, immunosensor technology, applied in chemical instruments and methods, material electrochemical variables, inorganic chemistry, etc., can solve the problems of time-consuming, low accuracy, etc., and achieve lower detection limits, good catalytic performance, and good catalytic performance. performance effect

Inactive Publication Date: 2019-07-26
SHANDONG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional immunoassay technology has the disadvantages of time-consuming and low accuracy. Therefore, electrochemical immunosensors have the characteristics of miniaturization and portability. Elec

Method used

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Examples

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Embodiment 1

[0033] Example 1 A method for preparing an electrochemical immunosensor of PdCu nanowire functionalized porous graphene

[0034] (1) A glassy carbon electrode with a diameter of 3.0 mm is covered with Al 2 o 3 Polish the polishing powder into a mirror surface, and clean it ultrasonically in anhydrous ethanol;

[0035] (2) Add 6.0 µL, 0.5 mg / mL dispersion of PdCu nanowire functionalized porous graphene nanoparticles onto the electrode surface, rinse with ultrapure water, and dry at room temperature;

[0036] (3) Add 6.0 µL, 5.0 µg / mL hepatitis B e antibody dropwise to the electrode surface, rinse the electrode surface with pH=7.0 phosphate buffer solution, and dry it in a refrigerator at 4 °C;

[0037] (4) Continue to add 3.0 µL, 1.0 mg / mL bovine serum albumin solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse the electrode surface with pH=7.0 phosphate buffer solution, and store in a refrigerator at 4 °C dry in ...

Embodiment 2

[0039] Example 2 A method for preparing an electrochemical immunosensor of PdCu nanowire functionalized porous graphene

[0040] (1) A glassy carbon electrode with a diameter of 4.0 mm is covered with Al 2 o 3 Polish the polishing powder into a mirror surface, and clean it ultrasonically in anhydrous ethanol;

[0041] (2) Add 6.0 µL, 1.0 mg / mL dispersion of PdCu nanowire functionalized porous graphene nanoparticles onto the electrode surface, rinse with ultrapure water, and dry at room temperature;

[0042] (3) Add 6.0 µL, 10.0 µg / mL hepatitis B e antibody dropwise to the electrode surface, wash the electrode surface with phosphate buffer solution with pH=7.0, and dry it in a refrigerator at 4 °C;

[0043] (4) Continue to add 3.0 µL, 1.5 mg / mL bovine serum albumin solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse the electrode surface with pH=7.0 phosphate buffer solution, and place in a refrigerator at 4 °C dry...

Embodiment 3

[0045] Example 3 A method for preparing an electrochemical immunosensor of PdCu nanowire functionalized porous graphene

[0046] (1) A glassy carbon electrode with a diameter of 5.0 mm is covered with Al 2 o 3 Polish the polishing powder into a mirror surface, and clean it ultrasonically in anhydrous ethanol;

[0047] (2) Add 6.0 µL, 2.0 mg / mL dispersion of PdCu nanowire functionalized porous graphene nanoparticles onto the electrode surface, rinse with ultrapure water, and dry at room temperature;

[0048] (3) Add 6.0 µL, 15.0 µg / mL hepatitis B e antibody dropwise to the surface of the electrode, wash the electrode surface with phosphate buffer solution with pH=7.0, and dry it in a refrigerator at 4 °C;

[0049] (4) Continue to add 3.0 µL, 2.0 mg / mL bovine serum albumin solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse the electrode surface with pH=7.0 phosphate buffer solution, and store in a refrigerator at 4 ...

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Abstract

The invention belongs to the technical field of immunoassay and biosensing, and provides a preparation method of an electrochemical immunosensor of PdCu nanowire functionalized porous graphene. The PdCu nanowire functionalized porous graphene is used as an electrochemical signal amplification platform to construct a marker-free electrochemical immunosensor, so that quantitative detection of hepatitis B e antigen is achieved, the preparation method of the electrochemical immunosensor of the PdCu nanowire functionalized porous graphene has the advantages of high specificity, high sensitivity, low detection limit and the like, and has important scientific significance and application value for detection of hepatitis B.

Description

technical field [0001] The invention belongs to the technical fields of novel nanocomposite materials, immune analysis and biosensing, and provides a preparation method of a label-free electrochemical immunosensor of PdCu nanowire functionalized porous graphene, which is applied to the detection of hepatitis B e antigen HBeAg. Background technique [0002] At present, cancer is a major public health problem in many parts of the world. Cancer is usually only diagnosed after systemic metastasis. Cancer is not only fatal, but also one of the most expensive diseases in the world. The cure rate of advanced cancer is low, so the development of highly sensitive Quantitative detection of cancer pathogenic factors is of great significance to detect early cancer. [0003] Hepatitis B virus infection is one of the most concerned health problems. It can cause liver cancer, which ranks second in cancer mortality. Hepatitis B e antigen HBe Ag has been identified as a reliable tumor marker...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/576G01N33/543G01N27/327G01N27/26B82Y15/00B82Y30/00C01B32/194
CPCB82Y15/00B82Y30/00C01B32/194G01N27/26G01N27/3277G01N27/3278G01N33/5438G01N33/57484G01N33/5761
Inventor 刘青谭召灵刘会董慧颜芹董云会
Owner SHANDONG UNIV OF TECH
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