Preparation method and application of electrochemical immunosensor of PdCu nanowire functionalized porous graphene
A porous graphene, immunosensor technology, applied in chemical instruments and methods, material electrochemical variables, inorganic chemistry, etc., can solve the problems of time-consuming, low accuracy, etc., and achieve lower detection limits, good catalytic performance, and good catalytic performance. performance effect
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Embodiment 1
[0033] Example 1 A method for preparing an electrochemical immunosensor of PdCu nanowire functionalized porous graphene
[0034] (1) A glassy carbon electrode with a diameter of 3.0 mm is covered with Al 2 o 3 Polish the polishing powder into a mirror surface, and clean it ultrasonically in anhydrous ethanol;
[0035] (2) Add 6.0 µL, 0.5 mg / mL dispersion of PdCu nanowire functionalized porous graphene nanoparticles onto the electrode surface, rinse with ultrapure water, and dry at room temperature;
[0036] (3) Add 6.0 µL, 5.0 µg / mL hepatitis B e antibody dropwise to the electrode surface, rinse the electrode surface with pH=7.0 phosphate buffer solution, and dry it in a refrigerator at 4 °C;
[0037] (4) Continue to add 3.0 µL, 1.0 mg / mL bovine serum albumin solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse the electrode surface with pH=7.0 phosphate buffer solution, and store in a refrigerator at 4 °C dry in ...
Embodiment 2
[0039] Example 2 A method for preparing an electrochemical immunosensor of PdCu nanowire functionalized porous graphene
[0040] (1) A glassy carbon electrode with a diameter of 4.0 mm is covered with Al 2 o 3 Polish the polishing powder into a mirror surface, and clean it ultrasonically in anhydrous ethanol;
[0041] (2) Add 6.0 µL, 1.0 mg / mL dispersion of PdCu nanowire functionalized porous graphene nanoparticles onto the electrode surface, rinse with ultrapure water, and dry at room temperature;
[0042] (3) Add 6.0 µL, 10.0 µg / mL hepatitis B e antibody dropwise to the electrode surface, wash the electrode surface with phosphate buffer solution with pH=7.0, and dry it in a refrigerator at 4 °C;
[0043] (4) Continue to add 3.0 µL, 1.5 mg / mL bovine serum albumin solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse the electrode surface with pH=7.0 phosphate buffer solution, and place in a refrigerator at 4 °C dry...
Embodiment 3
[0045] Example 3 A method for preparing an electrochemical immunosensor of PdCu nanowire functionalized porous graphene
[0046] (1) A glassy carbon electrode with a diameter of 5.0 mm is covered with Al 2 o 3 Polish the polishing powder into a mirror surface, and clean it ultrasonically in anhydrous ethanol;
[0047] (2) Add 6.0 µL, 2.0 mg / mL dispersion of PdCu nanowire functionalized porous graphene nanoparticles onto the electrode surface, rinse with ultrapure water, and dry at room temperature;
[0048] (3) Add 6.0 µL, 15.0 µg / mL hepatitis B e antibody dropwise to the surface of the electrode, wash the electrode surface with phosphate buffer solution with pH=7.0, and dry it in a refrigerator at 4 °C;
[0049] (4) Continue to add 3.0 µL, 2.0 mg / mL bovine serum albumin solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse the electrode surface with pH=7.0 phosphate buffer solution, and store in a refrigerator at 4 ...
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