A super-resolution microscopy system for detection of small-sized plasmonic nanoparticles

Abstract

The invention discloses a super-resolution microscope system for detecting small-sized plasma nanoparticles, which is mainly composed of a first laser, a filter unit, a first silver reflector, a phase plate, a second laser, a polarization-dependent beam splitter, and a second laser. Three lasers, dichroic mirror, transflective glass, galvanometer unit, microscope objective, translation stage, second silver mirror, filter, first pinhole, photomultiplier tube, lock-in amplifier and terminal ; Wherein, the Gaussian suppressed light produced by the first laser is input to the filter unit, and the Gaussian suppressed light is input to the first silver reflector and the phase plate after the filter unit, and the Gaussian suppressed light is generated by the phase plate to generate ring-shaped suppressed light; the lock-in amplifier controls the first The second laser produces Gaussian modulated light. The invention has the characteristics of low suppressed power, ultra-high resolution, no photobleaching, high signal contrast, extremely high repeatability, and durable observation, and can realize small-sized plasmonic nanometers in complex biological environments. Microscopic imaging of particles.

Description

technical field [0001] The invention relates to the technical field of super-resolution microscopic imaging, in particular to an all-optical switch regulation technology based on the nonlinear characteristics of small-sized plasma nanoparticles and a weak signal extraction technology of a lock-in amplifier. Background technique [0002] Plasmonic nanoparticles have a surface plasmon effect due to the presence of a large number of freely oscillating electrons on the surface, and are widely used in optical devices, biomarkers, solar cells, non-volatile memory, nanotechnology and other fields. At the same time, small-sized plasmonic nanoparticles In terms of material characterization and biomicroscopic imaging, it has the advantages of small size and obvious plasma effect. At present, there are mainly three types of detection methods for small-sized plasmonic nanoparticles. The first type mainly uses the interaction between particles and materials to perform small detection, su...

AI Technical Summary

Problems solved by technology

At present, there are mainly three types of detection methods for small-sized plasmonic nanoparticles. The first type mainly uses the interaction between particles and materials to perform small detection, such as atomic force microscopy (AFM) using the interaction force between atoms, and the interaction between electrons and matter. Scanning electron microscope (SEM) and transmission electron microscope (TEM), etc.; the second type of method mainly uses the scattering characteristics of nanoparticles for observation, such as dark field microscopy (DFM); the third type uses fluorescent substances for specific Indirect detection with markers is mainly based on stimulated radiation depletion (STED), photoactivated localization microscopy (PALM) and structured illumination microimaging (SIM). However, the above detection methods are in practical application There are problems such as high sample preparation requirements (first category), low detection resolution (second category), and unstable signal (third category).

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