A rapid detection method of lead ions based on fluorescence colorimetry and microfluidic technology
A microfluidic technology and detection method technology, which is applied in the field of heavy metal safety detection in aquatic products, can solve the problems of cumbersome synthesis process, low accuracy, and high price of fluorescent sensing materials, and achieve improved selectivity, photostability, and energy saving. Assay cost, effect of reducing reagent and sample consumption
Active Publication Date: 2021-08-03
JIANGSU UNIV
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Problems solved by technology
However, the existing fluorescent colorimetric method for detecting heavy metal ions has two shortcomings: one is that the synthesis process of fluorescent sensing materials is cumbersome, the amount used is large and the price is expensive; the other is that the detection results are obtained by artificially comparing the fluorescent color with a standard color comparison card , although the detection cost is low and the operation is convenient, but the accuracy is low and the error is large
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Embodiment 1
[0039] Step 1: Preparation of fluorescent probe; 100 mg of silk was placed in a 10 mL porcelain bowl, 6 mL of polyethylene glycol (PEG200) and 200 μL of 98% concentrated H 2 SO 4 , mixed and stirred for 10min, microwave heated for 15s, and the power of the microwave oven was 900W, and the solution changed from colorless to golden yellow, which indicated the successful preparation of carbon quantum dots (carbon quantum dots); the prepared carbon quantum dots were surface-modified with active groups, with Reduction; then take 5mL carbon quantum dot solution in a 15mL beaker, add 5mL Cu(NO 3 ) 2 solution (20mM), and adjust the pH to 10, magnetically stirred for 12h, the solution was light purple, and a carbon quantum dot-copper nanocluster solution was prepared. This system has dual emission fluorescence characteristics at an excitation wavelength of 365nm, and the fluorescence peaks are 440nm and 550nm , corresponding to copper nanoclusters and carbon quantum dots, respectivel...
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Abstract
The invention belongs to the technical field of heavy metal safety detection in aquatic products, and specifically relates to a rapid detection method for lead ions based on fluorescence colorimetry and microfluidic technology; the specific steps are: preparation of fluorescent probe carbon quantum dot-copper nano cluster solution, The preparation of the microfluidic chip and the construction of the image acquisition platform are used to collect the fluorescence color image of the microfluidic chip, the acquisition of fluorescence information, the construction of the quantitative model and the detection of the actual sample through the dark box image acquisition device; the present invention uses carbon quantum dots ‑Copper nano-cluster nanocomposite system combined with microfluidic technology to prepare microfluidic chips, which reduces the consumption of reagents and samples, greatly saves detection costs, and improves the detection efficiency of lead ion content; , The combination of microfluidic chip technology and digital image processing technology realizes the rapid visual detection of lead ions in aquatic products. Compared with other rapid detection methods, it has the advantages of high detection accuracy and strong stability.
Description
technical field [0001] The invention belongs to the technical field of heavy metal safety detection in aquatic products, and in particular relates to a rapid detection method for lead ions based on fluorescence colorimetry and microfluidic technology. Background technique [0002] With the rapid development of social economy, heavy metal elements are widely used in various fields of production and life, such as electronic manufacturing, agriculture and metallurgy. Because heavy metal elements are difficult to degrade, they are easy to accumulate in the environment and cause ecological environment damage. In natural water bodies, heavy metal elements will be absorbed and accumulated by aquatic organisms through bioaccumulation, and ingested by the human body through the food chain. Studies have shown that long-term intake of excessive heavy metals can damage the liver, kidneys and nervous system, and endanger human health. Lead ion (lead ion) is a typical heavy metal pollut...
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IPC IPC(8): G01N21/78G01N21/64
CPCG01N21/643G01N21/78
Inventor 李文亭石吉勇邹小波史永强胡雪桃石海军黄晓玮李志华
Owner JIANGSU UNIV