Hybridoma cell line 6b1 and its secreted monoclonal antibody against foot-and-mouth disease type a virus and its application

Abstract

The invention discloses a hybridoma cell strain 6B1, a foot-and-mouth disease A type virus resisting monoclonal antibody secreted from the hybridoma cell strain 6B1, and an application of the antibody, and belongs to the technical field of biology. The hybridoma cell strain can continuously and stably secrete the foot-and-mouth disease A type virus resisting monoclonal antibody, through the monoclonal antibody, various strains of a foot-and-mouth disease A type virus can be specifically recognized, and the monoclonal antibody has the advantages of being high in specificity and high in sensitivity. A colloidal gold test strip and a test card based on the monoclonal antibody as well as a reagent kit by an indirect ELISA method are good and high in sensitivity when being used for detecting the specificity of an antigen or an antibody of the foot-and-mouth disease A type virus. The monoclonal antibody can exert important effects on detecting the foot-and-mouth disease A type virus, monitoring the vaccine production quality and researching epidemiology and has great application prospects.

Description

technical field [0001] The invention belongs to the hybridoma cell line and its secreted monoclonal antibody and application in the field of biotechnology, in particular to the hybridoma cell line 6B1 and its secreted monoclonal antibody against foot-and-mouth disease A virus and the antibody's ability to detect foot-and-mouth disease A Type virus antibody and antigen application. Background technique [0002] Foot-and-mouth disease is an acute, febrile, and contagious infectious disease common to cloven-hoofed animals caused by Foot-and-mouth disease virus (FMDV) infection. The most susceptible animals are cattle, pigs, and sheep. Because foot-and-mouth disease spreads rapidly, is difficult to control, and has few remedial measures, it is called the "number one killer" of animal husbandry. The prevention of foot-and-mouth disease in my country is mainly through vaccination, and those who have foot-and-mouth disease are hunted and killed. [0003] There are 7 serotypes of ...

AI Technical Summary

Problems solved by technology

In addition, the transportation of disease materials may also lead to potential biological transmission risks

[0005] CN105950563A discloses a hybridoma cell line 7E3 and its secreted anti-foot-and-mouth disease type A virus monoclonal antibody and its application. The specificity of the monoclonal antibody 7E3 specifically involved is good, but it can only detect the virus strain Re-A / WH / 09, but cannot detect strains AKT58, AKTIII and AF72, so the detection spectrum is narrow

CN107541500A discloses a type A foot-and-mouth disease virus monoclonal antibody and its application, specifically mentioning a monoclonal antibody 2A2, which is a specific monoclonal antibody for foot-and-mouth disease type A, but it does not indicate which type A virus strain it is suitable for (such as Re-A / WH / 09, AKT58, AKTIII or AF72, etc.), so the detection spectrum of this monoclonal antibody is not clear

In order to obtain the ideal effect of the competition method, the amount of antigen and antibody must be controlled. Excessive amount will lead to low sensitivity; insufficient amount will lead to poor specificity

The indirect ELISA method also faces a problem of high-purity whole virus antigen. If the antigen purity is not good, the specificity of the test result will become poor.

Although conventional liquid chromatography (such as molecular sieves, ion exchange, affinity purification, etc.) can purify whole viruses, the operation is complicated and requires specialized instruments (high performance liquid chromatography, etc.) and reagents (various purification columns), And in the purification process, it is easy to cause the cracking of the whole foot-and-mouth disease virus

Images