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Method for extracting chondroitin sulfate and collagen peptide from animal cartilage

A chondroitin sulfate and collagen peptide technology, which is applied in the production process and the extraction of collagen peptides by enzymatic hydrolysis, can solve the problems of suboptimal production methods and high production costs, and achieve the effects of white color, improved benefits, and increased economic benefits.

Pending Publication Date: 2019-08-20
SHANDONG HAIYU BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production cost of chondroitin sulfate manufacturers using enzymatic hydrolysis is relatively high, and the yield and purity are improved compared with alkaline hydrolysis, but the production method is not ideal (the product yield is relative to the content of cartilage itself)

Method used

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  • Method for extracting chondroitin sulfate and collagen peptide from animal cartilage
  • Method for extracting chondroitin sulfate and collagen peptide from animal cartilage
  • Method for extracting chondroitin sulfate and collagen peptide from animal cartilage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A preparation method utilizing animal cartilage to produce chondroitin sulfate and collagen peptides respectively, comprising the steps of:

[0065] (1) Cartilage treatment: Put 4500Kg of fresh bovine nasal bone into a 10000L reactor, add purified water to 9000L, and adjust the temperature of the feed liquid to 36.9°C.

[0066] (2) Use acetic acid to adjust the pH value of the feed solution to 4.5, extract at 36.9°C for 8 hours (use acetic acid to maintain the pH value of 4.5±0.2 during the extraction process), and after the extraction is completed, transfer the extract solution and filter it with a plate and frame Machine filter to get the extract, and the extract enters the next process. The remaining cartilage goes to another process.

[0067] Extraction treatment:

[0068] (2a) Concentrate the extract by ultrafiltration until the volume of the filtrate is 1 / 4 of the volume of the extract, then transfer the filtrate to an alcohol precipitation tank, start stirring ...

Embodiment 2

[0079] A preparation method for extracting chondroitin sulfate and collagen peptides from animal cartilage, comprising the steps of:

[0080] (1) Cartilage treatment: Put 4500Kg of fresh pig nose bones into a 10000L reactor, add purified water to 9000L.

[0081] (2) Adjust the temperature of the feed liquid to 36.8°C, adjust the pH value of the feed liquid to 4.4 with acetic acid, and extract for 8 hours (use acetic acid to maintain the pH value of 4.5±0.2 during the extraction process), after the extraction is completed, transfer the extract to a plate The extract is filtered by frame filter and enters the next process. The remaining cartilage goes to another process.

[0082] Extraction treatment:

[0083] (2a) Concentrate the extract by ultrafiltration with an ultrafiltration machine until the volume of the filtrate is 1 / 4 of the volume of the extract, stop the concentration, transfer the filtrate to the alcohol precipitation tank, start stirring and add ethanol (concentr...

Embodiment 3

[0094] A preparation method for extracting chondroitin sulfate and collagen peptides from animal cartilage, comprising the steps of:

[0095] (1) Cartilage treatment: put 4500Kg of fresh shark bone into a 10000L reactor, add purified water to 9000L.

[0096] (2) Adjust the temperature of the feed liquid to 37.1°C, adjust the pH value of the feed liquid to 4.6 with acetic acid, and extract for 8 hours (keep the pH value of the feed liquid at 4.5±0.2 during the extraction process). Extract the liquid and filter it with a plate and frame filter to obtain the extract, and enter the next process. The remaining cartilage goes to another process.

[0097] Extraction treatment:

[0098] (2a) Concentrate the extract by ultrafiltration until the volume of the filtrate is 1 / 4 of the volume of the extract, transfer the filtrate to an alcohol precipitation tank, start stirring and add ethanol (concentration: 92.2%) to The concentration of ethanol in the alcohol precipitation system was ...

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Abstract

The invention discloses a preparation method for respectively extracting chondroitin sulfate and collagen peptide from animal cartilage (cattle, pigs, sharks and the like). The preparation method comprises the following steps of performing leaching and extraction on animal cartilage raw materials with acetic acid, performing ultrafiltration and concentration on raffinate to obtain a concentrated solution A, performing ethanol precipitation, refining, filtration, ethanol precipitation, dehydration and drying on the concentrated solution A to obtain the chondroitin sulfate; and performing hydrolysis on surplus cartilage ingredients in a reaction kettle with proteases, then performing filtration, adding activated carbon, performing adsorption and filtration, performing nanofiltration and concentration, performing double-effect concentration, and performing spray drying so as to obtain collagen polypeptide. According to the preparation method disclosed by the invention, the high-value utilization of cartilage resources is realized, the chondroitin sulfate and the collagen peptide can be extracted and separated efficiently at the same time, the added value of products can be increased,and environmental pollution is reduced.

Description

technical field [0001] The invention relates to a processing technology of animal cartilage, in particular to a method for extracting chondroitin sulfate and collagen peptides respectively by using animal cartilage, in particular to extracting chondroitin sulfate with acetic acid extraction and then extracting collagen peptides with enzymatic hydrolysis A production process. Background technique [0002] my country is a populous country with relatively developed agriculture and animal husbandry and rich cartilage resources. Active ingredients such as chondroitin sulfate and collagen rich in animal cartilage, throat bone, nasal bone and trachea have been widely used in the fields of medicine, health food, cosmetics and food. [0003] Chondroitin Sulfate (CS) is an acidic mucopolysaccharide composed of repeating disaccharide units composed of D-glucuronic acid and N-acetylgalactosamine. It contains 50-70 disaccharide basic structures with a molecular weight between 10,000-50...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/14C07K1/34C08B37/08
CPCC12P21/06C07K14/78C08B37/0069C08B37/0003
Inventor 李爱菊赵艳凯王怀雷张志强
Owner SHANDONG HAIYU BIOLOGICAL CO LTD
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