Laboratory mouse adipose tissue paraffin section tissue processing method

Abstract

The invention relates to a laboratory mouse adipose tissue paraffin section tissue processing method and belongs to the pathological tissue section manufacturing technology field. A traditional adipose tissue processing scheme takes a long time and is inefficient. In the invention, the above problems are solved. The method comprises the following steps of S1, fixing laboratory mouse in vitro adipose tissues in a neutral buffered formalin solution with a concentration of 10% for 24 hours, and then taking out the in vitro adipose tissues for later use; and S2, successively using following gradient concentration ethanol solutions to dehydrate the in vitro adipose tissues, and acquiring the dehydrated in vitro adipose tissues which are used for subsequent embedding. Time for manufacturing a laboratory mouse adipose tissue paraffin section in the method of the invention is shortened by 16 hours compared with the time in a traditional program, and working efficiency is greatly improved. Thesection is complete without cavities and wrinkles. Under a microscope, an adipose tissue morphology is intact, cells are full, nuclei are clear, a cell membrane has no fracture and no shrinkage, and ahigh quality section basis is provided for subsequent scientific research such as HE staining, immunohistochemistry and the like.

Description

technical field [0001] The invention belongs to the technical field of pathological tissue preparation, and in particular relates to a method for treating experimental mouse adipose tissue paraffin section tissue. Background technique [0002] Adipose tissue is an important energy storehouse of the body, which widely exists in the subcutaneous and internal organs of the whole body. Adipose tissue is mainly composed of adipocytes containing lipid droplets. In recent years, scientists have paid more and more attention to the study of adipose tissue, such as the study of adipose-derived tumors, liver diseases, etc. These diseases first need us to study through experimental animals, and this is inseparable from morphological aspects (such as HE staining , immunohistochemistry) observations. Therefore, it is very important to make good sections of adipose tissue specimens. [0003] However, due to the special structure of adipose tissue, which contains a large amount of oil, i...

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Problems solved by technology

[0003] However, due to the special structure of adipose tissue, which contains a large amount of oil, if the tissue is not completely fixed and dehydrated, it is difficult to make a complete tissue paraffin section

When making paraffin sections in routine laboratory procedures, various problems such as tissue fragmentation, cavities, soft tissue, and depression after placement often occur, and if the adipose tissue is developed according to the general tissue temperature (45°C). slices, the temperature is too high, the slices are easy to disperse, and the slices cannot be picked up, which will affect the subsequent staining, observation and research

Although many experiences and methods have been reported in the literature, making adipose tissue paraffin sections according to the traditional process described in the literature can produce relatively complete adipose tissue paraffin sections, which basically meet the requirements of morphological research, but the whole process takes a long time and the efficiency is obvious Reduced, slowed down the process of scientific research

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