Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

a t 1 -t 2 Dual nuclear magnetic resonance imaging contrast agent and preparation method and application thereof

A nuclear magnetic resonance imaging, T1-T2 technology, applied in the direction of pharmaceutical formulations, preparations for in vivo tests, emulsion delivery, etc., to achieve the effects of small particle size, easy purification, and simple preparation methods

Active Publication Date: 2022-05-17
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention has mild reaction conditions, simple operation process, and is easy to separate and purify; the Fe prepared by the present invention 3 o 4 Magnetic nanoparticles have good colloidal stability, biocompatibility and tumor targeting, but they can only be used for T 2 weighted imaging

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • a t <sub>1</sub> -t <sub>2</sub> Dual nuclear magnetic resonance imaging contrast agent and preparation method and application thereof
  • a t <sub>1</sub> -t <sub>2</sub> Dual nuclear magnetic resonance imaging contrast agent and preparation method and application thereof
  • a t <sub>1</sub> -t <sub>2</sub> Dual nuclear magnetic resonance imaging contrast agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] This embodiment provides a T 1 -T 2 A method for preparing a dual-nuclear magnetic resonance imaging contrast agent, the method comprising the steps of:

[0072] (1) Dissolve 1 g of carboxymethyl chitosan in 50 mL of deionized water to obtain a carboxymethyl chitosan solution with a concentration of 20 g / L, place it in a three-necked flask, feed nitrogen gas for 30 minutes, and heat the oil bath to 102 °C Obtain reaction system A, wherein the molecular formula of carboxymethyl chitosan is:

[0073]

[0074] Wherein, n is any integer in 100-500;

[0075](2) Take FeCl 3 ·6H 2 O and FeCl 2 4H 2 O is dissolved in hydrochloric acid solution with a hydrogen ion concentration of 1mol / L, FeCl 3 ·6H 2 O and FeCl 2 4H 2 The molar ratio of O is 2:1.5;

[0076] (3) Inject solution B into reaction system A, slowly add ammonia water with a mass fraction of 25 wt.% to adjust the pH to 10, and reflux at 100° C. for 100 minutes to obtain reaction system C;

[0077] (4) Co...

Embodiment 2

[0091] This embodiment provides a T 1 -T 2 A method for preparing a dual-nuclear magnetic resonance imaging contrast agent, the method comprising the steps of:

[0092] (1) Dissolve 1g of carboxymethyl chitosan in deionized water to obtain a carboxymethyl chitosan solution with a concentration of 15g / L, place it in a three-necked flask, feed nitrogen gas for 40min, and heat the oil bath to 110°C to obtain Reaction system A, wherein the molecular formula of carboxymethyl chitosan is:

[0093]

[0094] Wherein, n is any integer in 100-500;

[0095] (2) Take FeCl 3 ·6H 2 O and FeCl 2 4H 2 O is dissolved in a hydrochloric acid solution with a hydrogen ion concentration of 1.5 mol / L, FeCl 3 ·6H 2 O and FeCl 2 4H 2 The molar ratio of O is 2:1.2.

[0096] (3) Inject solution B into reaction system A, slowly add ammonia water with a mass fraction of 27 wt.% to adjust the pH to 11, and reflux at 110° C. for 90 minutes to obtain reaction system C;

[0097] (4) Cool the re...

Embodiment 3

[0103] This embodiment provides a T 1 -T 2 A method for preparing a dual-nuclear magnetic resonance imaging contrast agent, the method comprising the steps of:

[0104] (1) Dissolve carboxymethyl chitosan in deionized water to obtain a carboxymethyl chitosan solution with a concentration of 18g / L, place it in a three-necked flask, feed nitrogen gas for 40 minutes, and heat the oil bath to 90°C to react System A, wherein the molecular formula of carboxymethyl chitosan is:

[0105]

[0106] Wherein, n is any integer in 100-500;

[0107] (2) Take FeCl 3 ·6H 2 O and FeCl 2 4H 2 O dissolved in a sulfuric acid solution with a hydrogen ion concentration of 2 mol / L, FeCl 3 ·6H 2 O and FeCl 2 4H 2 The molar ratio of O is 2:1.8.

[0108] (3) Inject solution B into reaction system A, slowly add ammonia water with a mass fraction of 28 wt.% to adjust the pH to 11, and reflux at 90° C. for 80 minutes to obtain reaction system C;

[0109] (4) Cool the reaction system C to 25°...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle sizeaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of nuclear magnetic resonance and relates to a T 1 -T 2 A dual-nuclear magnetic resonance imaging contrast agent and its preparation method and application. The contrast agent includes ferric iron tetroxide magnetic nanoparticles coated with carboxymethyl chitosan. The particle diameter of the magnetic nanoparticles is 5-25nm, and the hydrated particles are The diameter is 20‑200nm, the contrast agent has superparamagnetism and prominent T 1 with T 2 The relaxation enhancement effect has a good long-term circulation effect. After the function is fully exerted, it can be metabolized by the body. The preparation method is simple, the conditions are mild, and the cost is low. The contrast agent provided by the invention can be freeze-dried and stored in solid form. Long shelf life, little change in properties when reconstituted with deionized water.

Description

technical field [0001] The invention belongs to the technical field of nuclear magnetic resonance, relates to a contrast agent and its preparation method and application, in particular to a T 1 -T 2 Dual-nuclear magnetic resonance imaging contrast agent and its preparation method and application. Background technique [0002] According to the type of imaging enhancement, the currently used MRI contrast agents are divided into two categories: positive contrast agents and negative contrast agents. They respectively enhance the 1 / T of the local area 1 or 1 / T 2 Relaxation efficiency to achieve the effect of image signal enhancement to brighten or darken the picture. The commonly used negative contrast agent is iron, but many of them are unstable in nature due to large particle size, or can only be stable in the organic phase due to the limitation of the preparation method, which makes the application limited. [0003] In addition, most contrast agents only have one contrast...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/12A61K49/18
CPCA61K49/126A61K49/1863
Inventor 陈春英刘涛周会鸽赵宇亮
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com