Regenerated cellulose filament for artificial ligament and application of regenerated cellulose filament

A technology for regenerating cellulose and artificial ligaments, which can be used in cellulose/protein conjugated man-made filaments, ligaments, tissue regeneration, etc., and can solve problems such as unfavorable growth of surrounding tissues, difficult cells, and excessively dense ligament weaving. Achieving favorable effects on cell migration and matrix formation, improving histocompatibility, and good histocompatibility

Inactive Publication Date: 2019-08-23
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of PET molecules is highly symmetrical, and the low surface energy makes it difficult for cells to attach and enter the interior of PET fibers. The osteoinductivity is poor, and there is a problem of poor bone healing between the graft and the host, and its biological activity is still unsatisfactory.
Its poor mechanical properties lead to densely woven ligaments and small fiber gaps, which are also not conducive to the growth of surrounding tissues.

Method used

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  • Regenerated cellulose filament for artificial ligament and application of regenerated cellulose filament
  • Regenerated cellulose filament for artificial ligament and application of regenerated cellulose filament
  • Regenerated cellulose filament for artificial ligament and application of regenerated cellulose filament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of silk fibroin solution:

[0029] Put 200g silk at a temperature of 100°C and 4-6L Na with a mass concentration of 1g / L 2 CO 3 Degumming in the solution for 3 times, 30-40min each time, remove the sericin protein to obtain the degummed silk fibroin protein, then wash the obtained silk fibroin protein with deionized water, and dry it at 50-60°C to constant weight for later use .

[0030] Calcium chloride-ethanol-water (CaCl 2 -EtoH-H 2 O) solution, silk fibroin is dissolved in calcium chloride-ethanol-water (CaCl 2 -EtoH-H 2 (0) solution, dissolve the degummed silk fibroin in a constant temperature water bath at 60°C for 4-5h, after dissolving, after the silk fibroin solution is cooled to room temperature, use a centrifuge at 8 000-10000r / min, centrifuge for 5-10min, Obtain silk fibroin solution.

Embodiment 2

[0032] At room temperature, take 100ml 20% (mass volume fraction) silk fibroin protein solution, add 50g polyvinyl alcohol, 30mg chondroitin sulfate and stir until completely dissolved, then add 20g type I collagen and stir slowly until dissolved to form a spinning solution. Wet spinning technology is used to prepare regenerated fiber filaments. The spinning process is as follows: the spinning solution is squeezed into the spinneret through a pressure pump at a room temperature of 20-25°C; the spinning solution coming out of the spinneret enters the coagulation bath, the coagulation bath is 10% alcohol, and the length of the coagulation tank is 1m; Pressure 0.1MPa; spinning rate 5mL / h; winding rate 8r / min. Through the comparison of spinning process parameters, coagulation bath is the most influential factor, followed by spinning speed, and pressure is the least influential factor. Through water and alcohol coagulation bath experiments with different concentrations, when the c...

Embodiment 3

[0034] At room temperature, take 100ml 25% (mass volume fraction) silk fibroin protein solution, add 50g polyvinyl alcohol, 30mg chondroitin sulfate and stir until completely dissolved, then add 15g type I collagen and slowly stir until dissolved to form a spinning solution. Wet spinning technology is used to prepare regenerated fiber filaments. The spinning process is as follows: the spinning solution is squeezed into the spinneret through a pressure pump at a room temperature of 20-25°C; the spinning solution coming out of the spinneret enters the coagulation bath, the coagulation bath is 10% alcohol, and the length of the coagulation tank is 1m; Pressure 0.1MPa; spinning rate 5mL / h; winding rate 8r / min. The regenerated fiber filament prepared in this embodiment is named NF2.

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Abstract

The invention relates to a regenerated cellulose filament for an artificial ligament. The regenerated cellulose filament comprises type-I collagen, silk fibroin, polyvinyl alcohol and chondroitin sulfate. In the raw materials of the regenerated cellulose filament, the mass ratio of the type-I collagen to the silk fibroin to the polyvinyl alcohol to the chondroitin sulfate is (15-20):(20-25):(40-50):(0.02-0.03). The artificial ligament can be further prepared. The prepared regenerated cellulose filament is composed of the collagen and the silk fibroin, the histocompatibility of the cellulose filament is greatly improved, and for cells, the regenerated cellulose filament not only has no toxicity, but also facilitates apposition growth of the cells; after the prepared artificial ligament is transplanted, the growth of autologous ACL sample cambiums are more easily generated in the body, and fusion of the cambiums and the ligament is tighter.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and relates to regenerated cellulose filaments for artificial ligaments and applications thereof. Background technique [0002] With the increasing national strength and the improvement of living standards in our country, the incidence of ligament injury is increasing year by year. Taking the most common anterior cruciate ligament (ACL) injury as an example, the annual number of ligament injuries in my country is conservatively estimated at more than 10 million. The anterior cruciate ligament is the inner ligament of the knee joint, which connects the tibia and the femur, controls the rotation of the knee joint and prevents excessive anterior displacement of the tibial plateau or posterior displacement of the femur. The importance of its function lies in maintaining and coordinating the stability of the knee joint It has stable performance, helps the human body to complete a variety of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/58A61F2/08D01C3/02D01D5/06D01F1/10D01F8/02D01F8/10
CPCA61F2/08A61F2240/001A61L27/26A61L27/58A61L2430/10D01C3/02D01D5/06D01F1/10D01F8/02D01F8/10C08L89/00C08L29/04C08L5/08
Inventor 王富友张颖陈光兴杨柳
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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