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Method for preparing high productivity mushroom beta-glucan and products thereof

A technology of glucan and manufacturing method, which is applied in the field of manufacturing to increase the yield of mushroom β-glucan, can solve the problems of poor homogenization and ineffective utilization, and achieve the effect of increasing the yield of polysaccharides

Active Publication Date: 2019-08-23
陈秀男
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the homogenization degree of the mycelium cell breaking technology in the previous case TW481718 is not good, and the small molecule ecology, glycoprotein, polysaccharide or other functional molecules inside the mushroom mycelium cannot be effectively utilized.

Method used

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  • Method for preparing high productivity mushroom beta-glucan and products thereof
  • Method for preparing high productivity mushroom beta-glucan and products thereof
  • Method for preparing high productivity mushroom beta-glucan and products thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0052] The present invention also provides a method for preparing high-purity mushroom β-glucan powder, comprising: adding an excipient to the mushroom β-glucan prepared by the above-mentioned preparation method to form a compound; and drying the compound .

[0053] The drying step includes spray drying or freeze drying to dry the compound.

[0054] The excipients include lactose, sucrose, glucose, fructooligosaccharide, starch or starch derivatives, and dietary fiber. In a specific embodiment, the excipient is dietary fiber. In another specific embodiment, the dietary fiber includes dextrin, and the dextrin is especially preferably Fibersol-2. Because the Fibersol-2 has good water solubility and good adsorption capacity for polysaccharides, the concentration of mushroom β-glucan powder in the mushroom β-glucan powder can be effectively increased through drying treatment, especially when spray drying is used. The moisture content of the processed mushroom β-glucan powder pr...

Embodiment 1 to 6

[0071] Embodiment 1 to 6: the test that culture fluid composition influences polysaccharide body output

[0072] One liter of culture liquid is prepared in a fermentation vessel with sterile water as a solvent, and trehalose, mannose and glucose are used as carbon sources of the culture liquid, and the weight ratio of the trehalose, mannose and glucose is 1:1:1, Use 0.5% by weight of yeast extract as the nitrogen source of the culture solution; then, put the configured production medium together with the fermentation vessel into a high-pressure steam sterilizer for high-temperature and high-pressure sterilization, and the sterilization conditions are 121 ° C, 15 minutes for sterilization Afterwards, cool at room temperature, then add the ganoderma lucidum mycelium, and cultivate for about 15 days at a speed of 30 to 100 rpm, so that the mushroom mycelium can synthesize a large amount of mushroom β-glucan in the culture solution.

[0073] The fermented culture solution containi...

Embodiment 4

[0075] The treatment method of Example 4 is the same as that of Example 1, but the mannose of the culture medium is changed to mannitol as shown in Table 1, and the result of the composition of the culture medium on the yield of mushroom β-glucan is evaluated.

[0076] The treatment methods of Examples 5 to 6 are the same as in Example 4, but the types of mushrooms are changed to Schizophyllum and Antrodia Cinnamomea as shown in Table 1, and the results of the composition of the culture solution on the yield of mushroom β-glucan are evaluated.

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Abstract

A method of preparing a high yield of mushroom beta-glucan is provided. The method includes: providing a liquid culture to culture the mushroom mycelium by fermentation, to increase the yields of themushroom mycelium and polysaccharide, wherein the liquid culture comprises at least two ingredients selected from the groups consisting of glucose, trehalose, a dietary fiber and mannose or derivatives thereof; and rupturing the mushroom mycelium with a continuous multiple-ultrasonic equipment; and removing insoluble matters from the liquid culture. A method of preparing highly pure mushroom beta-glucan powder and solution and the products thereof are also provided. By the method of the present disclosure, the yield of mushroom beta-glucan is effectively increased, its activity loss is reduced, and the stability of product thereof is improved.

Description

technical field [0001] The invention relates to a method for producing novel polysaccharides, in particular to a method for increasing the yield of mushroom β-glucan. Background technique [0002] Many recent studies have confirmed that the metabolites of mushrooms contain quite a few physiologically active components, including β1-3, 1-6 glucans, proteins, triterpenoids, nucleic acids, lectins, biological Substances such as alkali, antibiotics and steroids have immune regulation, anti-inflammation, anti-oxidation, inhibition of platelet aggregation and anti-tumor effects, among which polysaccharides can activate immune cells to enhance the body's immunity, so its medical application is the most It has attracted much attention, and the combined use of β-glucan and anticancer drugs by means of subcutaneous, intraperitoneal and intravenous injection is the trend of cancer treatment in the future. [0003] The current commercially available dextran products are made by using g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
CPCC12P19/04C12N13/00C08B37/0024A01G18/40C12N2500/34C12N1/14
Inventor 陈秀男
Owner 陈秀男