Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Enterobacter mori and application thereof in degrading tahiamethoxam

A technology of Enterobacter mulberry and thiamethoxam, applied in the field of microbiology and food safety

Active Publication Date: 2019-08-27
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, Enterobacter mulberry has been reported as a pathogenic bacteria (Meng Jiaorong et al., Biological characteristics and control agent screening of mulberry bacterial wilt in Guangxi [J]. Guangxi Plant Protection, 2015,28(3):1-7.), There is no literature report on the degradation of pollutants or pesticides, and there is no report on the degradation of neonicotinoid insecticides by Enterobacter mulberry

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enterobacter mori and application thereof in degrading tahiamethoxam
  • Enterobacter mori and application thereof in degrading tahiamethoxam
  • Enterobacter mori and application thereof in degrading tahiamethoxam

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation, identification and degradation function verification of Enterobacter mulberry TMX-13

[0029] 1. Isolation and identification of strain Enterobacter mori sp.TMX-13

[0030] In September 2016, the applicant collected the roots of mulberry plants artificially sprayed with thiamethoxam from the mulberry field in the south of the Jiangsu Academy of Agricultural Sciences, and then sterilized the surface of the mulberry roots, ground them into juice, and applied them on the mulberry plant containing thiamethoxam. On the inorganic salt solid medium as the only carbon and nitrogen source, a plant endophyte strain with thiamethoxam degradation characteristics was screened. The applicant named the bacterium as TMX-13, and its electron microscope pictures are as follows: figure 1 As shown, the strain was rod-shaped, without endophytic spores.

[0031] The TMX-13 strain was identified as Enterobacter mori through physiological and biochemical characteristics ...

Embodiment 2

[0037] The preparation of embodiment 2 TMX-13 microbial agent

[0038] In the present embodiment, TMX-13 bacterial agent is obtained through the following steps:

[0039] A) Inoculate the bacterial strain TMX-13 obtained in Example 1 into LB medium, streak at 30°C, pick a single colony and cultivate it twice, pick a single colony in the strain activation medium, shake at 30°C, 150-220rpm Bed shaking culture for 12-24 hours to obtain activated strains;

[0040] B) Inoculating the activated strain into a fermenter equipped with a seed medium, the inoculum amount is 1% of the volume of the seed medium, at 25-38° C., and culturing in air for 16-24 hours to obtain liquid seeds;

[0041] C) inoculating liquid seeds into a fermenter equipped with seed medium, the inoculum amount is 1% of the volume of the seed medium, and cultivating at 30-35° C. in the dark at 200 rpm until the logarithmic growth phase to obtain a viable cell culture;

[0042] D) Take 50ml of live bacterial cultur...

Embodiment 3

[0043] Example 3 TMX-13 inoculum root irrigation and inoculation to restore thiamethoxam contamination of leafy vegetables

[0044] Sampling the natural soil of farmland, first exposed to the sun and air-dried, and then passing through a 30-mesh sieve to remove oversized stone particles, the soil composition properties are pH 5.97, organic matter content 66.8g / kg dry soil, total nitrogen content 0.36%, total carbon content The hydrogen content is 5.7174%, the total sulfur content is 0.01%, the oxygen content is 44.37%, the clay content is 2.33%, the silt content is 16.1%, and the sand content is 81.5%.

[0045] Soak all the roots of the three-leaf and one-hearted small green vegetable Shanghai Qingming in the TMX-13 bacterial agent obtained in Example 2 for 4 hours, then transplant it to the natural soil of the farmland above, and soak the control group in physiological saline; after 21 days of cultivation, Shanghai Qingming is mature , sprayed thiamethoxam pesticide (purchase...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a strain Enterobacter mori and application thereof in degrading tahiamethoxam, and the preservation number of the Enterobacter mori is CGMCC No.16236. The Enterobacter mori can be prepared into a microbial agent, the microbial agent can be applied to the repair of a soil source with tahiamethoxam residual pollution in a leafy vegetable planting system, lower tahiamethoxamresidual in leaves of leafy vegetables, and promote the growth of plants; the Enterobacter mori belongs to soil probiotics, can be planted in the vegetables and plants for a long term, and can efficiently reduce the tahiamethoxam residual on the premise of not influencing vegetable quality, and realize green and safe production.

Description

technical field [0001] The invention relates to a strain of Enterobacter mori and its application in degrading thiamethoxam, that is, the bacteria prepared by using the Enterobacter mori for the soil polluted by the soil source plasticizer thiamethoxam in the leaf vegetable planting system Agents and their application belong to the field of microbiology and food safety. [0002] technical background [0003] The development of the vegetable industry plays an irreplaceable role in the optimization and adjustment of the planting industry structure in our country, the increase of agricultural efficiency, the increase of farmers' income, and the expansion of export earnings. Vegetables have a short growth period, fast growth, and high multiple cropping index, and are extremely vulnerable to pests and diseases during the production process. According to incomplete statistics, there are more than 200 kinds of vegetable pests in my country. A large amount of pesticides are used eve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C09K17/14A01N63/00A01P21/00B09C1/10C12R1/01
CPCB09C1/10C09K17/14A01N63/00C12R2001/01C12N1/205
Inventor 万群李易芯徐文君余向阳
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com