Method for removing oil and fat from donkey-hide gelatin peptide

A gelatin peptide and oil technology, applied in the biological field, can solve the problems of cumbersome processing process, high cost, long time, etc., and achieve the effects of high clearance rate, low cost and low cost

Pending Publication Date: 2019-08-27
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of this method is too cumbersome and takes a long time
[0006] The sample processing process of the above method is c...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] ① Compound enzymolysis method to enzymatically hydrolyze donkey-hide gelatin

[0033] Add dried donkey-hide gelatin lumps into the buffer solution, heat to prepare a concentration of 110g (dry) / L, and adjust the initial pH to 7.5; according to the enzymatic activity unit of trypsin, alkaline protease and neutral protease at 1:1:4, add Trypsin, alkaline protease and neutral protease, add 13000U per gram of dried donkey-hide gelatin, and enzymolyze at 50°C for 110 minutes to obtain hydrolyzate;

[0034] The dried donkey-hide gelatin lump is an intermediate product of the concentration process in the production process of donkey-hide gelatin, with a water content of 13.1% and a protein content of donkey-hide gelatin of 86.8%;

[0035] Described buffer solution is the disodium hydrogen phosphate-sodium hydroxide solution of pH7.5;

[0036] The compound enzyme method is to carry out synchronous enzymolysis after mixing the added trypsin, alkaline protease and neutral protea...

Embodiment 2

[0046] ① Compound enzymolysis method to enzymatically hydrolyze donkey-hide gelatin

[0047] Add dried donkey-hide gelatin lumps into the buffer solution, heat to prepare a concentration of 110g (dry) / L, and adjust the initial pH to 7.5; according to the enzymatic activity unit of trypsin, alkaline protease and neutral protease at 1:1:4, add Trypsin, alkaline protease and neutral protease, add 13000U per gram of dried donkey-hide gelatin, and enzymolyze at 50°C for 110 minutes to obtain hydrolyzate;

[0048] The dried donkey-hide gelatin lump is an intermediate product of the concentration process in the production process of donkey-hide gelatin, with a water content of 13.1% and a protein content of donkey-hide gelatin of 86.8%;

[0049] Described buffer solution is the disodium hydrogen phosphate-sodium hydroxide solution of pH7.5;

[0050] The compound enzyme method is to carry out synchronous enzymolysis after mixing the added trypsin, alkaline protease and neutral protea...

Embodiment 3

[0060] ① Compound enzymolysis method to enzymatically hydrolyze donkey-hide gelatin

[0061] Add dried donkey-hide gelatin lumps into the buffer solution, heat to prepare a concentration of 110g (dry) / L, and adjust the initial pH to 7.5; according to the enzymatic activity unit of trypsin, alkaline protease and neutral protease at 1:1:4, add Trypsin, alkaline protease and neutral protease, add 13000U per gram of dried donkey-hide gelatin, and enzymolyze at 50°C for 110 minutes to obtain hydrolyzate;

[0062] The dried donkey-hide gelatin lump is an intermediate product of the concentration process in the production process of donkey-hide gelatin, with a water content of 13.1% and a protein content of donkey-hide gelatin of 86.8%;

[0063] Described buffer solution is the disodium hydrogen phosphate-sodium hydroxide solution of pH7.5;

[0064] The compound enzyme method is to carry out synchronous enzymolysis after mixing the added trypsin, alkaline protease and neutral protea...

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PUM

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Abstract

The invention discloses a method for removing oil and fat from donkey-hide gelatin peptide, and belongs to the technical field of biology. A donkey-hide gelatin peptide product is obtained by taking adry donkey-hide gelatin heap as a raw material, performing enzymolysis on donkey-hide gelatin protein by using compound protease, degreasing and decolorizing by activated carbon, combining with an ultrafiltration separation and purification method, and performing spray drying. The method for removing the oil and fat from the donkey-hide gelatin peptide disclosed by the invention has the advantages of low cost, short clearing time, less pollution, high clearing rate, simple process and no influence on various functions of the donkey-hide gelatin peptide.

Description

technical field [0001] The invention relates to a method for removing fat from donkey-hide gelatin peptide, which belongs to the field of biotechnology. Background technique [0002] Collagen is an extremely important structural protein in connective tissue, widely present in animal tissues such as bone, tendon, muscle sheath, ligament, sarcolemma, cartilage and skin. Its extracted products have been widely used in medicine, health care, food processing, cosmetics and many other fields. The main source of raw materials for collagen products currently produced is the skin, bone and tendons of terrestrial mammals such as pigs or cattle. The removal of grease from animal hides remains an unresolved problem. [0003] In the production of collagen, defatting is part of the pretreatment. Too much fat content will generate insoluble calcium fatty acid in the subsequent process, and agglomerate, seriously hindering the subsequent operation. In addition, too much oil residue will...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/14C07K1/34
CPCC12P21/06C07K14/78
Inventor 王元秀查士银丁良
Owner UNIV OF JINAN
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