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Gel material for rapid detection of pesticide and preparation method and application thereof

A technology of gel materials and pesticides, which is applied in the field of gel materials and its preparation for rapid detection of pesticides. It can solve the problems of reducing SERS signal detection, not being able to load SERS effects on AuNPs, and affecting the effective contact of analytes. It achieves high detection sensitivity, Suitable for mass production, the effect of uniform and stable morphology

Active Publication Date: 2019-09-06
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the NPs core-PVA shell composites are directly prepared by chemical methods, on the one hand, a large amount of AuNPs cannot be loaded into the PVA gel to obtain excellent SERS effect; Its SERS signal detection

Method used

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  • Gel material for rapid detection of pesticide and preparation method and application thereof
  • Gel material for rapid detection of pesticide and preparation method and application thereof
  • Gel material for rapid detection of pesticide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Electrostatic adsorption-SERS technology detects the positively charged probe molecule 2-aminopyridine, and the negatively charged probe molecule benzoic acid. The detection flow chart is as follows figure 1 Shown:

[0058] A. Microchannel device preparation of PVA gel microspheres:

[0059] (1) Add 0.5 g of PVA and 500 μL of sodium alginate into a centrifuge tube to mix evenly, and ultrasonicate for 30 minutes to remove air bubbles as the water phase;

[0060] (2) Mix 0.5mL N,N-dimethylformamide and 200mL ethyl acetate as an oil phase solution;

[0061] (3) Using the microchannel device ( figure 2 ), the water phase is added to the syringe pump, the oil phase solution is added to the peristaltic pump, the water phase and the oil phase are pumped into the cross-shaped reactor respectively, and microspheres are formed by the action of shear force, and PVA gel is obtained after further solidification Microspheres: The flow rate of the water phase in the syringe pump: ...

Embodiment 2

[0092] Detection of the positively charged pesticides cartap and 2-aminoethylphosphonic acid, and the negatively charged pesticides 4-nitro-1H-pyrazole-3-carboxylic acid and 3,5-dichlorophenol in fish flesh:

[0093] figure 1 The positively charged pesticide cartap and 2-aminoethylphosphonic acid, the negatively charged pesticide 4-nitro-1H-pyrazole-3-carboxylic acid and The flowchart of 3,5-dichlorophenol, described detection method comprises the following steps:

[0094] (1) Pretreatment of fish samples

[0095] Positively charged pesticides cartap and 2-aminoethylphosphonic acid, negatively charged pesticides 4-nitro-1H-pyrazole-3-carboxylic acid and 3,5-dichlorophenol and their metabolites in fish References were extracted and purified and optimized for Hurtaud-Pessel and Verdo's methods. The specific steps are as follows: Weigh 2.00±0.02g of fish meat into a 50mL centrifuge tube, add 500μL of 9.5g / L hydroxylamine hydrochloride solution and place it in the dark for 10mi...

Embodiment 3

[0104] A gel material for rapid detection of pesticides, the preparation method of which is as follows:

[0105] A. Microchannel device preparation of PVA gel microspheres:

[0106] (1) Add 0.5 g of PVA and 500 μL of sodium alginate into a centrifuge tube to mix evenly, and ultrasonicate for 30 minutes to remove air bubbles as the water phase;

[0107] (2) Mix 0.5mL N,N-dimethylformamide and 200mL ethyl acetate as an oil phase solution;

[0108] (3) Using the microchannel device, the water phase is added to the syringe pump, the oil phase solution is added to the peristaltic pump, and the water phase and the oil phase are pumped into the cross-shaped reactor respectively, and microspheres are formed by the action of shear force. After solidification, PVA gel microspheres are obtained: the flow rate of the water phase in the syringe pump: 0.02mL / min, the flow rate of the oil phase in the peristaltic pump: initially set at 0.2mL / min, and then slowly adjust the flow rate to 10mL...

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Abstract

The invention relates to a gel material for rapid detection of a pesticide and preparation and application thereof. The preparation comprises the following steps of: (1) preparing PVA pellets; (2) assembling AgNPs on the surfaces of the PVA pellets to form an SERS (Surface Enhanced Raman Scattering) detection substrate; (3) modifying mercaptoethylamine and mercaptopropionic acid on the surfaces ofthe PVA@Ag pellets; (4) selectively absorbing positive and negative charge probe or pesticide molecules to the PVA@Ag surfaces by utilizing the electrostatic interaction; and (5) detecting the live probe molecules or pesticide absorbed to the PVA@Ag surfaces by utilizing a portable Raman spectrometer so as to implement qualitative and quantitative analysis detection on pesticide residues in aquatic products. Compared to the prior art, the PVA@Ag composite material obtained by the preparation disclosed by the invention has the excellent surface enhanced Raman activity, not only has the characteristics of rapid analysis, high sensitivity, small sample use amount, wide application range, simplicity and convenience for operation, convenience for carrying and the like, but also has selectivity, and does not need to be marked and subjected to separation purification.

Description

technical field [0001] The invention relates to the field of pesticide detection, in particular to a gel material for rapid detection of pesticides, a preparation method and application thereof. Background technique [0002] The development of agricultural industrialization has made the production of agricultural products more and more dependent on exogenous substances such as pesticides. The amount of pesticides used in agricultural products in my country remains high, but generally speaking, only 10%-20% of pesticides are attached to crops, while 80% are lost in soil, water and air, and are leached by irrigation or precipitation. Pollution of ground water. When the pesticides in the water body exceed the standard seriously, it will cause human disease, abnormal development, and even indirectly lead to poisoning death. At present, there are many kinds of rapid detection methods for pesticide residues in water, and their principles are mainly divided into two categories: bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/28
CPCG01N1/28G01N21/658
Inventor 李丹张钦梅邓维
Owner SHANGHAI INST OF TECH
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