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A kind of exosome sustained-release polypeptide hydrogel and preparation method and use thereof

A hydrogel and exosome technology, applied in the field of exosome slow-release polypeptide hydrogel, can solve problems such as short half-life, achieve slow release, prolong half-life, and have good clinical application prospects

Active Publication Date: 2022-03-18
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Exos has shown great potential for the treatment of AKI, its half-life in the blood circulation system after injection into the body is very short, and it is easily cleared by macrophages in the body. Therefore, it is necessary to design a slow-release carrier to deliver Exos to enhance its curative effect

Method used

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  • A kind of exosome sustained-release polypeptide hydrogel and preparation method and use thereof
  • A kind of exosome sustained-release polypeptide hydrogel and preparation method and use thereof
  • A kind of exosome sustained-release polypeptide hydrogel and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of ExOSomes of the MSCS source of the present invention

[0034] The preparation method is as follows:

[0035] 1, MSCS primary culture: mouse is broken, 70% alcohol soaked for 10 minutes, separating bilateral tibia, rinsing bone marrow with serum-free medium, collecting rinsing liquid, centrifuging, and using an adhesive method to obtain MSCs.

[0036] 2. Culture Collection: DMEM medium (US GBICO) developing MSCs (US GBICO) was cultured in DMEM medium (US GBICO), US GBICO, US SBI, and regular collection.

[0037] 3, centrifugal extraction: Extraction of EXOS, 2000 g × 10 minutes by multiple centrifugation methods, and remove cell fragments.

[0038] 4, purification: The last EXOS was cleaned once with PBS, and then centrifuged 10,000 degrees for 60 minutes again.

Embodiment 2

[0039] Example 2 Preparation of a polypeptide hydrogel of the present invention

[0040] According to the treatment of the present invention, the hydrogel sustained EXOS is used, and the sustained release system is determined according to the pre-test results (therapeutic effect) and the experimental animal (C57 mouse) and the application site (mouse kidney film) as follows:

[0041] Element Concentration (mg / ml) Volume (μL) EXOSMES (EXOS) 8 10 Self-assembled short peptide KMP2 10 13 Sterile water 10 3

2 total 25

[0042] (1) Self-assembled short peptide KMP2 (KLDLPVGLIGKLDL) was synthesized from Shanghai Potati Biotechnology, and dried from dry powder in sterile water, and was prepared from 10 mg / ml of reservoir, and 13 μL was added to the 1.5 mL of EP tube;

[0043] (2) Take 10 μl of concentration of 8 mg / ml EXOS to EXOS to add EP tube, mix well.

[0044] (3) 2 μl of sterile water, blow mixing mixed.

[0045] Example 2 Preparation...

Embodiment 3

[0048] Example 3 Preparation of Polypeptide Watergels of Cogens

[0049] (1) Self-assembled short peptide KMP2 (KLDLPVGLIGKLDL) is purified by Shanghai Potati Biotechnology, and 300 ug self-contained peptide is dissolved in 20 μl of water into a self-assembled peptide aqueous solution; 100 ug of exosomes are dissolved in 15 μL of water formation into an outer body Aqueous solution; add in the EP tube;

[0050](2) The self-assembled peptide solution obtained from step (1), an extractive solution, add 5 μl of water, a total of 40 μl, mix average, and a polypeptide hydrogel.

[0051] The beneficial effects of the present invention are proved by specific pharmacodynamics experiments:

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Abstract

The invention discloses a polypeptide hydrogel, which is a polypeptide hydrogel prepared by mixing a self-assembling peptide (SAP) aqueous solution and exosomes (Exosomes, Exos). The invention also discloses the preparation method and application of the aforementioned polypeptide hydrogel. The polypeptide hydrogel of the present invention can effectively slow down the release of Exos, prolong its half-life, and the released Exos has similar activity to the newly isolated Exos, can effectively reduce HK2 cell damage caused by hypoxia, and promote the recovery of vascular endothelial damage; The invented polypeptide hydrogel can effectively reduce the expression of neutrophil gelatinase-associated apolipoprotein (NGAL) and the synthesis and secretion of renal extracellular matrix (ECM) by reducing renal cell apoptosis, inflammatory factors and renal injury marker protein-neutrophil gelatinase-associated apolipoprotein (NGAL), and can effectively reduce acute Kidney damage, slow down the role of renal fibrosis, clinical application prospects are good.

Description

Technical field [0001] The present invention relates to the field of biomaterials, and more particularly to an exosomental sustained release polypeptide hydrogel that promotes damaged kidney repair. Background technique [0002] Acute Kidney Injury, AKI is a common clinical emergency, with rapid decline in renal function as clinical characteristics, eventually leading to acute renal failure, and even cause other organ failure. AKI has a lot of factors, such as ischemia / reperfusion, surgical comparative agents, misconduct, corrugated muscle solubility, infection, etc. In recent years, the incidence of Aki has increased, and AKI's AKI has increased by 1% to 5%, rapidly. At present, in the process of clinical treatment, there is still a lack of effective AKI prevention and treatment, and after AKI, the patient has improved the kidney function, but it is difficult to achieve complete recovery. The incomplete repair of damaged kidneys will lead to a series of pathological changes su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K9/06A61P13/12
CPCC07K7/08A61K35/28A61K47/42A61K9/06A61P13/12
Inventor 刘敬平周毅洁程惊秋陆燕蓉陈又南
Owner WEST CHINA HOSPITAL SICHUAN UNIV