A foliar barrier agent for inhibiting the absorption and transport of heavy metal cadmium by wheat grains and its use, research methods and applications

A technology of wheat grains and barrier agents, which is applied in the field of environmental pollution control, can solve problems such as reduction, and achieve the effects of convenient use, mitigation of toxic effects, and wide sources

Inactive Publication Date: 2021-04-27
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] According to published reports, foliar barriers have a good effect on reducing the absorption and accumulation of heavy metals such as Cd, Pb, and As in crops. A highly targeted, foliar barrier agent that can effectively block the absorption and transport of heavy metal cadmium in wheat grains, and is easy to operate

Method used

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  • A foliar barrier agent for inhibiting the absorption and transport of heavy metal cadmium by wheat grains and its use, research methods and applications
  • A foliar barrier agent for inhibiting the absorption and transport of heavy metal cadmium by wheat grains and its use, research methods and applications
  • A foliar barrier agent for inhibiting the absorption and transport of heavy metal cadmium by wheat grains and its use, research methods and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: field experiment

[0040] Experimental material: Aikang 58 (Henan local variety)

[0041] Experimental design: The experimental field is a heavy metal-contaminated farmland located in Xinxiang City, Henan Province, with an area of ​​5m*60m, which is divided into 5 plots of 5m*11m, and the area of ​​each plot is 55m 2 , the interval between each cell is 1m. The basic physical and chemical properties of the experimental field are shown in Table 2. Eight treatments were set in the experiment, namely: control group (deionized water), lanthanum chloride 5mmol / L, sodium nitroprusside 5umol / L, EDTA-2Na 2mmol / L, L-cysteine ​​1mmol / L, boric acid 5mmol / L, ferrous sulfate 10mmol / L, foliar fertilizer diluted 100 times, the experimental field was divided into 5 plots, the interval between each plot was 1m, and each treatment was 5 repetitions. According to the random block design, each treatment point was marked with a label, and the spraying treatment was carried o...

Embodiment 2

[0047] Embodiment 2: pot experiment

[0048] Experimental material: low resistance 58

[0049] experimental design:

[0050] 1. Soil treatment: The experiment of wheat soil cultivation potted planting selected heavy metal-polluted soil in Qixia Mountain, Nanjing. After the soil was naturally dried, impurities such as straw and stones were picked out. The naturally air-dried soil was fully ground and mixed evenly, and potted for sowing. The basic physical and chemical properties of Qixia Mountain polluted farmland soil are shown in Table 3.

[0051] 2. Soil column preparation: The soil column has a diameter of 10cm and a height of 30cm, and is placed in a pot with a diameter of 12cm. The bottom of the soil column is wrapped with 3 layers of 60-mesh gauze, and two layers of filter paper are placed on it. There are 54 pots in total.

[0052] 3. Wheat sowing: Sow 30 complete and uniform wheat grains in each pot to ensure sufficient soil moisture.

[0053] 4. Thinning and finali...

Embodiment 3

[0061] Example 3: Wheat Pot Experiment Digestion by Part and Sample Heavy Metal Cd Content Analysis

[0062] Experimental design: In order to further explore the transshipment relationship between various parts of wheat plants, mature wheat plants were divided into grains, wheat husks, flag leaves, middle leaves, lower leaves, the first internode (near the root system), the second section 8 parts between the internode and the third internode, and HNO was applied to different parts of the wheat plant 3 : HClO 4 =87:13 (v; v) is completely digested, and the sample after digestion is analyzed and detected by ICP-OES sample, and the digestion process adopts national standard substance GBW (E) 100348 to carry out quality control, and its recovery rate is above 90%; SPSS and GraphPad software were used to analyze the effect of blockers on the transport of heavy metal Cd in different parts of wheat plants.

[0063] Discussion and analysis:

[0064] (1) As shown in the figure above...

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Abstract

The invention provides a blocking agent which is highly targeted to wheat grains and can efficiently block the absorption and translocation of heavy metal cadmium in wheat grains, and its active ingredient is EDTA‑2Na. The present invention also provides a method for using the foliar barrier agent. It is sprayed on cadmium-contaminated wheat leaves during the period of vigorous wheat growth and development, which can effectively and significantly block the absorption and transport of heavy metal cadmium in wheat grains, and the application frequency and application amount are small. , has no toxic side effects, does not affect the normal growth and development of wheat, is safe and non-toxic to the human body, and can effectively inhibit the absorption and transport of heavy metal cadmium by wheat grains, and reduce the accumulation of cadmium in wheat grains. The invention also provides a method for researching the blocking mechanism of the heavy metal Cd of the foliar blocking agent, and a new application of EDTA-2Na, which provides a good solution to the problem of cadmium pollution of wheat grains.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution control, and relates to a foliar barrier agent for inhibiting the absorption and translocation of heavy metal cadmium by wheat grains, its use method, research method and application. Background technique [0002] Cadmium (Cd) is a heavy metal element with strong biological toxicity. It has strong chemical activity in the environment and long-lasting toxicity. It is easy to enter the human body through crops and cause harm. In recent years, soil heavy metal cadmium (Cd) pollution has become a serious problem, posing a threat to crop production and food security. Agricultural inputs, sewage irrigation and atmospheric deposition are the main sources of Cd pollution in farmland. [0003] With the frequent incidents of "arsenic poisoning", "blood lead" and "cadmium rice", heavy metal pollution has become one of the most concerned public events. At present, heavy metals in crops are mos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/44A01P21/00
CPCA01N37/44A01G22/20
Inventor 陈亚华杨洋郑翔夏胜兰沈振国李芳柏杜臻杰
Owner NANJING AGRICULTURAL UNIVERSITY
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