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Method and system for processing micro-nano structures on single protein crystals by femtosecond laser

A femtosecond laser and micro-nano structure technology, applied in laser welding equipment, metal processing equipment, manufacturing tools, etc., can solve problems that do not involve three-dimensional processing of protein single crystals, cannot be applied to protein structure analysis and biomanufacturing fields, etc. Achieve the effect of high processing precision and small heat-affected zone

Inactive Publication Date: 2019-09-17
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the existing methods involves the three-dimensional processing of protein single crystals, and cannot be applied to the fields of protein structure analysis and biomanufacturing

Method used

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  • Method and system for processing micro-nano structures on single protein crystals by femtosecond laser
  • Method and system for processing micro-nano structures on single protein crystals by femtosecond laser
  • Method and system for processing micro-nano structures on single protein crystals by femtosecond laser

Examples

Experimental program
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Embodiment 1

[0031] Example 1: Femtosecond Laser Fabrication of Porous Arrays Inside Single Protein Crystals

[0032] (1-1) In the present embodiment, bovine insulin is used as the protein raw material, and the ingredients in the supersaturated solution are bovine insulin powder 10 mg / ml, sodium dihydrogen phosphate 2% (mass fraction),

[0033] (1-2) Take 2 μl of the solution prepared in (1-1) and drop it on the cover glass, hang it above the liquid pool containing 10% (mass fraction) sodium dihydrogen phosphate solution, and keep it airtight at 20°C 24h, until protein crystals of about 100 μm can be observed in the suspended droplets through a microscope.

[0034] This embodiment processes a lattice with a pitch of 20 μm inside the protein, as shown in the attached figure 2As shown in , 11 is a focused laser beam, 12 is a protein crystal, and the dots indicated by dotted ellipses are dot arrays to be processed. The laser used is a femtosecond laser with a central wavelength of 800nm ​​...

Embodiment 2

[0035] Example 2: Femtosecond laser processing of micro-nano structures on the surface of a single protein crystal

[0036] In this embodiment, egg white lysozyme is used as protein raw material, and the composition of the supersaturated solution is: egg white lysozyme powder 30 mg / ml, sodium chloride 2.5% (mass fraction), sodium acetate trihydrate 1.36% (mass fraction).

[0037] Take 2 μl of this solution and drop it on the cover glass, hang it above the liquid pool containing 10% (mass fraction) sodium chloride solution, keep it airtight and stand at 20°C for 24 hours, until it can be observed in the suspended droplet through a microscope Protein crystals around 100 μm in size.

[0038] This embodiment processes the processed micro-nano structure on the surface of the protein, as shown in the attached image 3 As shown in , 11 is a focused laser beam, 12 is a protein crystal, and the crystal surface with a dotted line pattern is the surface to be processed, which forms a ce...

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Abstract

The invention relates to a method and a system for processing micro-nano structures on single protein crystals by femtosecond laser, and belongs to the field of femtosecond laser processing. The method uses the femtosecond laser for processing the micro-nano structures on the single protein crystals, and is high in processing precision, small in heat influenced area and incapable of causing destruction of crystal structures and inactivation of proteins. The processing system can realize processing of the protein crystals with complex space positions on any inclined plane through three-axle translation control of a translation table. The method can process the surfaces or the internals of the single protein crystals to obtain the micro-nano structures, wherein the smallest size of the micro-nano structures is 1-10 microns; and the realized micro-nano structures comprise ablation pits, lattices, micro-channels and the like, and can be widely applied to such technical fields as protein chips and microfluidics.

Description

technical field [0001] The invention relates to a method and system for processing a micro-nano structure on a single protein crystal with a femtosecond laser, belonging to the field of femtosecond laser processing. Background technique [0002] At present, biomaterials such as proteins have many important applications in the fields of biomedicine, environmental science, drug analysis, tissue engineering, etc. One of the research hotspots at this stage is the production of biomaterial micro-nano structures. It has great application prospects and development potential in the functionalization of micro devices and biological materials. For example, the micropatterning of biomolecules on the surface of materials is very important for many practical applications such as surface biodiagnosis, biosensors and biomedical devices. Cell adhesion and growth have very important effects. A patented method for immobilizing proteins on solid surfaces (application number 201110346431.X) a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B23K26/36B23K26/402
CPCB23K26/36B23K26/402
Inventor 姜澜俞嘉晨闫剑锋张正
Owner TSINGHUA UNIV