Preparation method and application of targeting enzyme immobilized carrier based on magnetic molecular imprinting technology

Abstract

The invention discloses a preparation method and application of a targeted enzyme immobilized carrier based on a magnetic molecular imprinting technology. The method comprises the following steps of preparing ferric aminotetroxide; preparing boracic acid modified ferroferric oxide; preparing a molecular imprinting polymer layer by a sol-gel method; removing horseradish peroxidase combined on the molecular imprinting polymer; sealing non-specific adsorption residuals on the surface of the polymers to obtain horseradish peroxidase magnetic molecular imprinting polymers. The method provided by the invention has the advantages that the preparation process is simple; the cost is low; the obtained magnetic molecular imprinting polymers can be used for specifically recognizing the target substance of the horseradish peroxidase, and can also be used for fixing the horseradish peroxidase in complicated samples directly through adsorption; the formed immobilized enzyme can be used for detecting substances such as glucose, sarcosine, trioxypurine and cholesterol capable of being decomposed to produce H2O2.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to the preparation of targeted immobilized horseradish peroxidase and its carrier based on magnetic molecular imprinting technology. Background technique [0002] Immobilized enzyme technology is a type of technology that restricts or binds enzymes to a certain area of ​​a solid phase carrier under the condition of maintaining the catalytic activity of the enzyme. Compared with unimmobilized free enzymes, immobilized enzymes overcome the shortcomings of free enzymes while maintaining their high-efficiency, specificity and mild enzymatic reaction characteristics. It has the advantages of repeated use, continuous and controllable operation, and simple process. Immobilized enzymes have been rapidly developed and widely used in chemistry, biology, medicine, food science, and environmental science. The preparation methods of immobilized enzymes can be divided into two ...

AI Technical Summary

Problems solved by technology

[0004] At present, the imprinted polymer technology of small molecules has become mature, however, for water-soluble protein molecules (such as enzyme molecules), commonly used methods for preparation and recognition in organic phases, such as bulk method, in situ method and precipitation method, etc. , due to the poor resistance of the protein to organic solvents and acid and alkali, it is not applicable, which makes the preparation of enzyme molecularly imprinted polymers difficult.

There is no report on the application of molecularly imprinted polymers to immobilized enzymes

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