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Detection method for improving reaction microenvironment by using sodium dodecyl sulfate to carry out qualitative and quantitative detection on silver ions

A technology of sodium dodecyl sulfate and detection method, applied in the field of analysis and detection, can solve the problems of high requirements for technical personnel, complicated process, long time consumption, etc., and achieve the effects of easy aggregation and deterioration, low environmental requirements, and simple operation.

Pending Publication Date: 2019-09-17
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods are relatively mature, they still encounter many problems in practical application: complex preprocessing is required before detection, and the process is complex and time-consuming; The need for real-time in situ detection
However, currently developed Ag based on spectrophotometry + The design principle of the detection method is relatively complicated, and most of them use nanomaterials that need to be synthesized in multiple steps, which makes the reproducibility of the method insufficient, thus affecting the accuracy of detection

Method used

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  • Detection method for improving reaction microenvironment by using sodium dodecyl sulfate to carry out qualitative and quantitative detection on silver ions
  • Detection method for improving reaction microenvironment by using sodium dodecyl sulfate to carry out qualitative and quantitative detection on silver ions
  • Detection method for improving reaction microenvironment by using sodium dodecyl sulfate to carry out qualitative and quantitative detection on silver ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Using secondary water as a solvent, prepare a series of 500 μL of standard silver ion solutions with concentrations of 0.5, 4, 10, 20, 30, 36, 50, 100, 200, and 300 μM, and then pour them into each standard silver ion solution, respectively. Add 500 μL of phosphate buffer (20mM, pH 8.5) containing 1.0mg / mL SDS and 1.2mM TMB and shake and mix thoroughly, let stand for 10min, observe the color of the solution after the reaction corresponding to each standard silver ion solution (specific color development state See figure 1 in a to j); the sample tubes containing the corresponding post-reaction solutions of these 10 standard silver ion solutions are respectively numbered as a, b, c, d, e, f, g, h, i, j, from figure 1 It can be seen that the colors of the solutions in sample tubes a to j are all blue, and as the concentration of silver ions increases, the color of the solution in the entire reaction system becomes darker, and as the concentration of silver ions increases, ...

Embodiment 2

[0041] (1) Using secondary water as a solvent, prepare a series of 500 μL of standard silver ion solutions with concentrations of 0.5, 4, 10, 20, 30, and 36 μM, respectively, and then add 500 μL of 1.0 mg to each standard silver ion solution. / mL SDS and 1.2mM TMB phosphate buffer (20mM, pH 8.5) and fully shake and mix, let stand for 10min;

[0042] (2) detect respectively the visible light absorbance value of solution at 397nm place after the reaction corresponding to above-mentioned each standard silver ion solution at room temperature with ultraviolet spectrophotometer (solution ultraviolet-visible absorption spectrum changes with silver ion concentration as follows figure 2 Shown), thus obtain the silver ion concentration-absorbance value standard curve (such as image 3 Shown), further obtain the linear regression equation y=0.0314x+0.2693 of absorbance value and silver ion concentration, R 2 =0.996, wherein, y is the absorbance value, and x is the concentration of silv...

Embodiment 3

[0046] Embodiment 3: In order to explore the color development when different substances are contained in the detection method of the present invention and to determine the principle of the reaction, the inventors of the present invention measured solutions containing different substances. The measurement method is specifically:

[0047] ① Take 940 μL of phosphate buffer (10mM, pH 8.5), add 60 μL of 10mM TMB, shake and mix thoroughly, and let it stand for 20 minutes; then observe the discoloration of the solution, and then detect the reaction product of the solution at room temperature with a UV spectrophotometer at 397nm absorbance value at .

[0048] ② Take 840 μL of phosphate buffer (10mM, pH 8.5), add 60 μL of 10mM TMB and 100 μL of 5mg / mL SDS, shake and mix thoroughly, and let it stand for 20 minutes; then observe the discoloration of the solution, and then test it with a UV spectrophotometer at room temperature Next, detect the absorbance value of the solution reaction p...

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Abstract

The invention relates to a detection method for improving a reaction microenvironment by utilizing sodium dodecyl sulfate to carry out qualitative and quantitative detection on silver ions, and the method comprises the following steps of: adding 3,3',5,5'-Tetramethylbenzidine (TMB) and sodium dodecyl sulfate (SDS) into a solution to be detected under an alkaline condition; observing whether the solution turns blue or not by adopting a visual colorimetry; realizing qualitative analysis on the silver ions in the solution to be detected; and then, determining the visible light absorbance value of the reacted solution at 397 nm by utilizing an ultraviolet spectrophotometer, and realizing quantitative detection on the silver ions in the solution to be detected. Compared with the prior art, the whole detection process is simple to operate, short in time consumption, low in cost, low in environmental requirement and easy to realize in a field rapid detection process; the detection method has stable color reaction, and no turbidity or color fading phenomenon is found within 48 hours after the solution is colored; and in addition, the method also has strong anti-interference capability.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to a detection method for qualitative and quantitative detection of silver ions by using sodium dodecyl sulfate to improve the reaction microenvironment. Background technique [0002] Silver is a heavy metal widely used in modern industries such as automobiles, electronics, pharmaceuticals, photosensitive imaging and other industries. Trace amounts of silver ions have antibacterial activity, but high doses of silver ions will seriously pollute the environment and endanger people's health. Therefore, the establishment of a rapid and sensitive silver ion detection method is of great importance for water quality analysis, food safety, and clinical medical diagnosis. Practical significance. [0003] At present, the commonly used silver ion detection techniques mainly include plasma mass spectrometry (ICP-MS), atomic spectrometry, electrochemical method and fluorescence...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N21/78G01N1/28
CPCG01N21/33G01N21/78G01N1/28
Inventor 许惠凤朱希余丽双张诗琪陈婷婷王丽丽
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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