New uses of Pogostemon cablin volatile oil
A technology of volatile oil and patchouli, applied in the direction of application, chemicals for biological control, fungicides, etc., to achieve high efficiency, long-lasting control effect, and quality improvement effect
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Embodiment 1
[0019] Embodiment 1: the preparation of patchouli volatile oil
[0020] (1) Add 8 times the mass of water to 1000g of chopped patchouli, soak for 2 hours, and use steam to extract the volatile oil in patchouli by heating and distillation. ;
[0021] (2) The extracted fluid was dried with anhydrous sodium sulfate to remove the contained water to obtain the volatile oil of patchouli, which was put into a brown sample bottle, sealed, and stored at -20°C until use.
Embodiment 2
[0022] Embodiment 2: the activation of pathogenic bacteria
[0023] (1) Potato Dextrose Agar (PDA): 200g potato, 20g glucose, 20g agar, 1000mL distilled water;
[0024] (2) Purify and identify the pathogenic bacteria isolated from the roots and shoots of Panax notoginseng, and identify them as Fusarium solani, Pythium aphanidermatum, Cylindrocarpondestructans, Botrytis cinerea cinerea), Colletotrichum gloeosporioides and Rhizoctonia solani, the above strains were inoculated on PDA medium for activation, and after inoculation for 3 to 4 times, vigorously growing strains were taken for later use.
Embodiment 3
[0025] Embodiment 3: GC-MS measures the chemical composition of patchouli volatile oil
[0026] (1) GC conditions: Instrument model: Agilengt Technologies 7890B-5977B; Chromatographic column: HP-5MS30m×250μm×0.25μm;
[0027] (2) EI source ionization source temperature: 230°C; quadrupole temperature: 150°C; scanning range: 30-500m / z; inlet temperature: 285°C; split ratio 10:1; sample volume: 1μl; Energy: 70eV; temperature program of column oven: 50°C for 4min, 5°C / min to 120°C, 1°C / min to 180°C, 10°C / min to 280°C and hold for 16min; The retention time of the alkane continuous carbon (C9-C25) is calculated, and the retention time and mass spectrum of the compound are compared with the NIST17.L database to determine the final compound. The test results are shown in the table below;
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PUM
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