Preparation method and application of immobilized microbial agent for kitchen waste treatment
A technology for immobilizing microorganisms and kitchen waste, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc. Obvious odor and other problems, to achieve the effect of preventing a large loss of live bacteria, tight combination, and strong ability
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Embodiment 1
[0044] Example 1 Screening and Identification of Predominant Bacteria for Degrading Food Waste
[0045] Take 5g of the soil sample near the Yuxiu Restaurant of Zhejiang University of Technology and put it into a sterilized Erlenmeyer flask with small glass beads and 50mL of sterile water, shake at 30°C and 200r / min for 20min, and suspend the bacteria in the ultra-clean bench. Liquid gradient dilution, the concentration gradient is 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 , inoculate 100 μL of each concentration of bacterial suspension on a PDA plate, seal the plate with a sealing film and invert it, place it in a constant temperature incubator at 28-37°C for 24-48 hours, select a plate with a suitable number of colonies, and pick typical colonies , after separation and purification, inoculate them on protein, starch, fat, and cellulose differential medium plates and spread evenly with a sterilized coating rod. Seal the culture dish with a parafilm and place it ups...
Embodiment 2
[0069] The preparation of embodiment 2 microbial bacterial agents
[0070] 1. Preparation of unimmobilized microbial agents
[0071] (1) Preparation of composite bacteria
[0072] (1) Incline cultivation:
[0073] Pichia kluyveri ZJB-091 and Geotrichumsilvicola ZJB-092 were inoculated on PDA solid medium respectively;
[0074] Among them, Geotrichum linsheng was cultured at a temperature of 30° C. for 48 hours, and Pichia kluyveri was cultured at a temperature of 28° C. for 72 hours to activate the strains.
[0075] (2) Primary seed liquid culture:
[0076] The activated Pichia kluyveri and Geotrichum forestiata after the activation of step (1) are inoculated respectively in 250mL liquid culture medium, carry out seed culture, obtain Pichia kluyveri first grade seed liquid culture and Geotrichum forestiata grade one seed liquid culture;
[0077] Among them, Geotrichum linsheng was cultured at a temperature of 30° C. for 48 hours, and Pichia kluyveri was cultured at a temp...
Embodiment 3
[0103] This embodiment uses the different immobilized microbial agents prepared in Example 2 to perform a degradation test on kitchen waste:
[0104] (1) Take the leftover meals in the school cafeteria and sort them to remove solid waste such as bones, paper scraps, wooden chopsticks, and plastic;
[0105] (2) Add 15kg of sorted kitchen waste in the garbage disposer, get the microbial inoculum that embodiment 2 makes (respectively unimmobilized microbial inoculum, immobilized microbial inoculum 1 and immobilized microbial inoculum Agent II) 900g is put into the garbage disposer, after continuing to ferment for 24 hours, then add 10kg of the above-mentioned food waste every other day, for 10 consecutive days, the weight ratio of the total food waste added to the microbial agent is about 130:1 ;
[0106] The fermentation temperature is 55°C, and the fermentation time is 28 hours; during the fermentation process, stirring is carried out, and the stirring frequency is 5 minutes e...
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