Active fermentation object and preparation method and application thereof

A fermented product and active technology, applied in the field of active fermented product and its preparation, can solve problems such as the decrease of β-glucan content, achieve the effects of increased yield and purity, low production cost, and good application prospects

Active Publication Date: 2019-10-08
泉后(广州)生物科技研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] In order to solve the problem in the prior art that the β-glucan content of oat bran fermented by Schizophyllum significantly decreases, the present invention firstly provides a method for preparing an active fermented product containing β-glucan, which specifically includes the following step:

Method used

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  • Active fermentation object and preparation method and application thereof
  • Active fermentation object and preparation method and application thereof
  • Active fermentation object and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, active fermented product of the present invention and preparation method thereof

[0035] S1. Mix oat bran and water according to the material-to-liquid ratio of 1:10 (W / V, g / mL), then use protease, glucoamylase and amylase to carry out enzyme treatment in turn, and then sterilize after the enzyme treatment to obtain oat Bran culture fluid;

[0036] The steps of the enzyme treatment are as follows:

[0037] a1, protease treatment: adjust the pH value to 6-7, then add papain with 1% weight of oat bran culture solution, and bathe in 60°C water bath for 120min;

[0038] a2, glucoamylase treatment: adjust the pH value to 4.0-4.5, add 1% glucoamylase by weight of the oat bran culture solution, and bathe in 55°C water for 3 hours;

[0039] a3. Amylase treatment: add 1% α-amylase by weight of oat bran culture solution (the α-amylase is freeze-dried powder, its weight percentage is 10%), and bathe in 70° C. water for 40 minutes.

[0040] S2. Get the oat bran cu...

Embodiment 2

[0046] Embodiment 2, active fermented product of the present invention and preparation method thereof

[0047] S1, take oat bran and water and mix according to the ratio of material to liquid 1:25 (W / V, g / mL), then use protease, glucoamylase and amylase to carry out enzymatic treatment successively, carry out sterilization after enzymatic treatment, make oat Bran culture fluid;

[0048] The steps of the enzyme treatment are as follows:

[0049] a1, protease treatment: adjust the pH value to 6-7, then add ficin with 5% weight of oat bran culture solution, and bathe in water at 65°C for 90 minutes;

[0050] a2, glucoamylase treatment: adjust the pH value to 4.0-4.5, add 3% glucoamylase by weight of oat bran culture solution, and bathe in water at 60°C for 2 hours;

[0051] a3. Amylase treatment: add 5% α-amylase by weight of oat bran culture solution (the α-amylase is freeze-dried powder, its weight percentage is 10%), and bathe in 75° C. water for 30 minutes.

[0052] S2. Ge...

Embodiment 3

[0058] Embodiment 3, active fermented product of the present invention and preparation method thereof

[0059] S1. Mix oat bran and water according to the ratio of material to liquid 1:15 (W / V, g / mL), then use protease, glucoamylase and amylase to carry out enzyme treatment in turn, and then sterilize after the enzyme treatment to obtain oat Bran culture fluid;

[0060] The steps of the enzyme treatment are as follows:

[0061] a1, protease treatment: adjust the pH value to 6-7, then add papain with 3% weight of oat bran culture solution, and bathe in 60°C water bath for 100min;

[0062] a2. Glucoamylase treatment: adjust the pH value to 4.0-4.5, add 2% glucoamylase by weight of the oat bran culture solution, and bathe in water at 60°C for 2.5 hours;

[0063] a3. Amylase treatment: add 3% α-amylase by weight of oat bran culture solution (the α-amylase is freeze-dried powder, its weight percentage is 10%), and bathe in 75° C. water for 30 minutes.

[0064] S2. Get the oat br...

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Abstract

The invention belongs to the technical field of biological fermentation, and particularly relates to an active fermentation object, a preparation method of the active fermentation object, and application of the active fermentation object to cosmetics. The preparation method comprises the steps: S1 oat bran and water are taken to be mixed, then enzyme treatment is conducted, after enzyme treatment,sterilization is conducted, and an oat bran culture solution is prepared; S2 the oat bran culture solution is taken to be transferred into a fermentation tank, schizophyllum commune strains subjectedto three-stage culture are inoculated for fermentation, and a schizophyllum commune polysaccharide fermentation solution is obtained; and S3 the schizophyllum commune polysaccharide fermentation solution is taken to be filtered through a plate-and-frame filter press, a schizophyllum commune fermentation extracellular product is obtained, and then the schizophyllum commune fermentation extracellular product is micro-filtered and washed with water through a ceramic membrane till the clear and bright active fermentation object is obtained. The method is low in production cost, and simple and stable in process, and the obtained fermentation object containing active components such as oat beta-glucan and schizophyllum commune polysaccharide has the remarkable repairing and moisturizing effectson the skin, is high in stability, and has good application prospects in the field of the cosmetics.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to an active fermented product, its preparation method and its application in cosmetics. Background technique [0002] Glucan is a natural polysaccharide present in oats, barley, yeast and fungi, including 1,3-glucan, 1,4-glucan (with branched chains) and 1 , 6-glucan (without branching), they may be α-type or β-type. A large number of studies have shown that β-glucan has a significant effect on reducing blood fat and blood sugar and improving the body's immunity, making it of great application value in biology, medicine, health care and food. [0003] In recent years, β-glucan has received a lot of research as a skin care ingredient. High polysaccharides of β-glucan can stimulate Langerhans cells, promote the proliferation of fibroblasts (dermis cells) and the synthesis of skin tissue matrix (such as collagen, elastin, proteoglycans and other component...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/08A61K8/9794A61K8/73A61Q19/00C12R1/645
CPCA61K8/73A61K8/9794A61K2800/805A61K2800/85A61Q19/00C12P19/08
Inventor 刘露张娇韩志东
Owner 泉后(广州)生物科技研究院有限公司
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