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Electrode material for aptamer sensor, aptamer sensor and preparation method thereof

An aptamer sensor and electrode material technology, which is applied in the direction of material analysis, material analysis, and material electrochemical variables by electromagnetic means, which can solve the problems of low chemical and physical stability, high antibody production cost, and difficult chemical modification. , to achieve the effect of high sensitivity and selectivity, good selectivity, and excellent comprehensive performance

Active Publication Date: 2019-10-15
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, previous electrochemical studies using Mb antibodies have several limitations, including low chemical and physical stability, high antibody production costs, batch-to-batch variability, and difficulty in chemical modification.

Method used

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  • Electrode material for aptamer sensor, aptamer sensor and preparation method thereof
  • Electrode material for aptamer sensor, aptamer sensor and preparation method thereof
  • Electrode material for aptamer sensor, aptamer sensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The electrode material for the aptasensor of this embodiment is prepared by the following steps:

[0066] (1) Manganese cobalt Prussian blue analogue

[0067] Disperse 150 mg of manganese acetate and 150 mg of sodium citrate into 20 mL of ultrapure water under stirring to obtain solution A.

[0068] 133 mg of K 3 [Co(CN) 6 ] was dissolved in 20mL of ultrapure water to obtain solution B.

[0069] Under stirring, solution A was poured into solution B and further stirred for 10 min, then stood at room temperature for 24 h to obtain a yellow precipitate. The precipitate was separated, washed with water and ethanol by centrifugation, and then dried at 70°C for 12 hours to collect the product, which was the manganese cobalt Prussian blue analogue, which was denoted as MnCo PBA.

[0070] (2) Electrode materials for aptamer sensors

[0071] Place the manganese-cobalt Prussian blue analog obtained in step (1) in a tube furnace at 5°C min -1 The heating rate was raised to 5...

Embodiment 2

[0073] The electrode material for the aptamer sensor of this embodiment differs from the preparation steps of the electrode material for the aptamer sensor of Example 1 only in that the calcination temperature in step (2) is different, and the electrode material for the aptasensor of the present embodiment The step (2) of is:

[0074] The manganese-cobalt Prussian blue analogue obtained in step (1) of Example 1 is placed in a tube furnace at 5° C. min -1 The heating rate was increased to 300°C, and the manganese-cobalt Prussian blue analogue was calcined for 2 hours, and then cooled to room temperature to obtain the electrode material for aptasensor, which was designated as Mn-Co-300.

Embodiment 3

[0076] The electrode material for the aptamer sensor of this embodiment differs from the preparation steps of the electrode material for the aptamer sensor of Example 1 only in that the calcination temperature in step (2) is different, and the electrode material for the aptasensor of the present embodiment The step (2) of is:

[0077] The manganese-cobalt Prussian blue analogue obtained in step (1) of Example 1 is placed in a tube furnace at 5° C. min -1 The heating rate was increased to 800°C, and the manganese-cobalt Prussian blue analog was calcined for 2 hours, and then cooled to room temperature to obtain the electrode material for aptasensor, which was designated as Mn-Co-800.

[0078] Two, the specific embodiment of the preparation method of the aptamer sensor of the present invention is as follows:

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Abstract

The invention relates to an electrode material for an aptamer sensor, the aptamer sensor and a preparation method, and belongs to the technical field of electromechanical sensors. The electrode material for the aptamer sensor is prepared according to a method comprising the following steps: (1) dissolving manganese salt, citrate and cobalt cyanide acid salt into a solvent; carrying out a reactionto obtain a manganese cobalt Prussian blue analogue; (2) roasting the obtained manganese cobalt Prussian blue analogue to obtain the electrode material for the aptamer sensor. The method is simple, high in operability, and low in cost; the prepared electrode material is high in electrochemical activity and high in combination effect with a nucleic acid aptamer material; the aptamer sensor preparedaccording to the method is high in selectivity, stability, repeatability, reproducibility and adaptability, and low in detection limit.

Description

technical field [0001] The invention relates to an electrode material for an aptamer sensor, an aptamer sensor and a preparation method thereof, belonging to the technical field of electrochemical sensors. Background technique [0002] Acute myocardial infarction (AMI) is the main cause of cardiac death in patients all over the world, and the rapid and accurate diagnosis of AMI has been paid more and more attention. Determination of the concentration of cardiac biomarkers such as serum myoglobin (Mb), troponin I (TnI), C-reactive protein (C-RP), creatine kinase MB (CK-MB) has become an accurate method for the diagnosis of AMI. Useful ways. Among these cardiac biomarkers, Mb is the first cardiac marker released after myocardial injury, which plays an important role in the early diagnosis of AMI. Mb is rapidly released into the blood circulation within 4-5 h after the onset of AMI, and its concentration will increase to about 4.8 μM, which is significantly higher than the no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01C3/08G01N27/30G01N27/327G01N27/48
CPCC01C3/08C01P2002/72C01P2002/82C01P2002/85C01P2004/03C01P2004/04G01N27/308G01N27/3275G01N27/3277G01N27/3278G01N27/48
Inventor 王明花宋英攀何领好张治红贾巧娟李真真
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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