Engineering strain for fermented synthesis of ergothioneine and construction method thereof

An engineering strain and ergothioneine technology, applied in the field of bioengineering, can solve the problems of expensive synthetic raw materials, high extraction cost and high synthetic cost, and achieve the effects of efficient biosynthesis and wide application value
CN110358719AActive Publication Date: 2019-10-22JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-10-22

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Abstract

The invention discloses an engineering strain for fermented synthesis of ergothioneine and a construction method thereof, and belongs to the technical field of bioengineering. With escherichia coli and bacillus subtilis as the host and three shuttle plasmids pEBS of bacillus subtilis, escherichia coli and saccharomyces cerevisiae as the expression vector, egtABCDE gene clusters from different sources are introduced into the escherichia coli and the bacillus subtilis for heterologous expression; biosynthesis of the ergothioneine is realized; then, the egtABCDE gene clusters are subjected to optimized expression, including codon optimization, promoter optimization and expression of different combinations, and meanwhile a culture medium and added methionine, hercynine, histidine and cysteineare optimized. Through the optimized expression and optimized culture conditions, efficient biosynthesis of the ergothione is realized. The engineering strain lays a foundation for efficient fermentedpreparation of ergothione by a microbial system and is suitable for industrial production and application.
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Description

technical field

[0001] The invention relates to an engineering strain for fermenting and synthesizing ergothioneine and a construction method thereof, belonging to the technical field of bioengineering. Background technique

[0002] Ergothioneine is a rare natural chiral histidine-derived thiol compound with unique biological functions and pharmacological activities. It not only has strong antioxidant activity: it can scavenge free radicals, peroxynitroso, hypochlorous acid, chelate divalent metal ions, activate antioxidant enzymes, inhibit superoxide dismutase, etc., but also It has anti-inflammatory and cell-protecting effects, and has broad application prospects and market prospects in the fields of medicine, food, health care products, cosmetics and biotechnology.

[0003] Ergothioneine was first isolated from ergot bacteria in 1909, and it can also be synthesized in microorganisms such as actinomycetes, cyanobacteria and fission yeast, some fungi, streptomyces, mycobac...

Claims

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