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Method for extracting soluble protein from discarded tobacco leaves

A waste tobacco leaf, soluble technology, applied in the direction of protein food ingredients, protein ingredients of plant materials, food science, etc., can solve the problems of hindering a large number of extraction steps, unsuitable for industrial extraction production, costing reagent costs, etc., to meet the requirements of green environmental protection , The extraction method is simple and fast, and the extraction efficiency is improved

Pending Publication Date: 2019-10-25
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current extraction methods of plant soluble protein mainly include direct heating precipitation method, alkali-soluble acid precipitation method, organic solvent method, fermentation method, etc. Although there are many extraction methods, there are high-speed or ultra-fast freezing methods in the traditional methods of extracting soluble protein. Steps such as centrifugation, gel filtration, isoelectric focusing and ultrafiltration that hinder a large number of extractions are complicated to operate, consume high reagent costs, and are not suitable for industrial extraction production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting soluble protein from waste tobacco leaves, comprising the following steps:

[0023] (1) Powdering: Weigh 1kg of tobacco leaf raw material, powder it with a pulverizer;

[0024] (2) Homogenization: Stir and mix the extracted tobacco leaf powder and extract evenly, then use a colloid mill to homogenate for 5 minutes, press and filter through a 200-mesh filter cloth to obtain a slurry;

[0025] (3) pH adjustment: adjust the pH of the filtered slurry to 8 with an alkaline reagent;

[0026] (4) Heat sink: put the alkaline liquid in a constant temperature water bath at 40°C for 20 minutes, take it out and let it stand for 1 day to obtain flocculent protein, and centrifuge to remove the supernatant to obtain protein precipitate 1.

[0027] (5) Washing and purification: Add 1L of washing solution to the obtained protein precipitate 1 and mix it thoroughly, then centrifuge to obtain protein precipitate 2, then add 1L of purifying agent and protein precip...

Embodiment 2

[0031] A method for extracting soluble protein from waste tobacco leaves, comprising the following steps:

[0032] (1) Powdering: Weigh 10kg of tobacco leaf raw material, powder it with a pulverizer;

[0033] (2) Homogenization: Stir and mix the extracted tobacco leaf powder and extract evenly, then use a colloid mill to homogenize for 7 minutes, press and filter through a 200-mesh filter cloth to obtain a slurry;

[0034] (3) pH adjustment: adjust the pH of the filtered slurry to 8.5 with an alkaline reagent;

[0035] (4) Heat sink: put the alkaline liquid in a constant temperature water bath at 50°C for 30 minutes, take it out and let it stand for 1 day to obtain flocculent protein, and centrifuge to remove the supernatant to obtain protein precipitate 1.

[0036] (5) Washing and purification: first add 5L of washing liquid to the obtained protein precipitation 1 according to a certain ratio of material to liquid and mix it thoroughly, then centrifuge to obtain protein prec...

Embodiment 3

[0040] A method for extracting soluble protein from waste tobacco leaves, comprising the following steps:

[0041] (1) Powdering: Weigh 100kg of tobacco leaf raw material and powder it with a pulverizer;

[0042] (2) Homogenization: Stir and mix the obtained tobacco leaf powder and the extract evenly, then use a colloid mill to homogenize for 9 minutes, press and filter through a 200-mesh filter cloth to obtain a slurry;

[0043] (3) pH adjustment: adjust the pH of the filtered slurry to 9.0 with an alkaline reagent;

[0044] (4) Heat sink: put the alkaline liquid in a constant temperature water bath at 60°C for 40 minutes, take it out and let it stand for 1 day to obtain flocculent protein, and centrifuge to remove the supernatant to obtain protein precipitation 1;

[0045] (5) Washing and purification: first add 20L of washing liquid to the obtained protein precipitation 1 according to a certain ratio of material to liquid and mix it thoroughly, then centrifuge to obtain pr...

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Abstract

The invention discloses a method for extracting soluble protein from discarded tobacco leaves. The method includes the steps: crushing the tobacco leaves into powder, and mixing the powder and extracts to prepare uniform slurry; adjusting filtered slurry to reach alkalinity to keep the slurry for a certain time at constant temperature; performing standing to obtain flocculating protein precipitation; washing and purifying protein; spray-drying the protein to obtain a soluble protein finished product. The method for extracting the soluble protein from the discarded tobacco leaves is simple, convenient and rapid, traditional steps preventing a lot of extraction such as high-speed or ultra-speed refrigerated centrifugation, gel filtration, isoelectric focusing and ultra-filtration are abandoned, protein extraction rate is larger than 65%, the protein after crude extraction is washed and purified, so that special smell of the soluble protein and parts of impurities in the discarded tobaccoleaves can be effectively removed, the purity of the soluble protein is improved, the method is suitable for large-scale industrial production, and broad prospects are provided for products such as animal feed developed by the soluble protein in the discarded tobacco leaves.

Description

technical field [0001] The invention belongs to the field of plant protein extraction, in particular to a method for extracting soluble protein from waste tobacco leaves. Background technique [0002] Flue-cured tobacco, a plant of the genus Nicotiana in the Solanaceae family, is the type of tobacco with the largest cultivation area in my country and the world. It is the main raw material for the cigarette industry and is also used as pipe tobacco. Studies have found that the protein content of flue-cured tobacco is higher than that of rice, wheat, corn and other crops. Among them, the protein content of flue-cured tobacco is about 10%, and that of sun-cured tobacco and Burley tobacco can be as high as 20%. However, due to technical limitations in the process of tobacco planting and cigarette production and processing, the leftovers (waste tobacco leaves) accounting for about 25% of the total mass of flue-cured tobacco will not be fully utilized and will be wasted in the ent...

Claims

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Application Information

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IPC IPC(8): A23J1/00
CPCA23J1/007
Inventor 苏贤坤吴拥军张婕陈旭峰杨慧李霓艺
Owner GUIZHOU TOBACCO SCI RES INST
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