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Method for preparing cordycepin by fermenting cordyceps militaris through calcium alginate freeze-dried network scaffold

A technology of calcium alginate and Cordyceps militaris, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of limiting production scale and production efficiency, and achieve improved production efficiency, small diffusion resistance, and large reaction efficiency effect

Active Publication Date: 2019-10-29
广州大鱼创福科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the liquid fermentation of Cordyceps is currently mainly static surface fermentation, which can only be performed intermittently, which limits the production scale and production efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Step 1: Treat and activate the spores of Cordyceps militaris through a plasma processor. The conditions of the plasma treatment are as follows: the gas uses oxygen, the processing power is 280W, the pressure is preferably 55Pa, and the processing time is 15min. Add 30g / L sodium alginate solution afterwards and make the Cordyceps militaris spore concentration reach 10 10 pieces / ml, stirred evenly, freeze-dried at -30°C, 0.024mBar into 3d scaffolds;

[0032] Step 2: Soak the scaffold in 20g / L calcium chloride solution for 3 days, carry out negative pressure flash explosion under 0.024mBar vacuum degree, and then carry out vacuum drying at 4°C and 0.024mBar to obtain the Cordyceps militaris fermented network scaffold;

[0033] Step 3: Cut the Cordyceps militaris fermentation network support into 1cm 3 Cubic packed column, the height-to-diameter ratio of the reaction column is 8:1, and the packed column volume is 80%. Prepare a conversion stock solution of 12g / L glucose, ...

Embodiment 2

[0035] Step 1: activate the spores of Cordyceps militaris through plasma processor treatment, the plasma treatment conditions are as follows: the gas is nitrogen, the treatment power is 250W, the pressure is 50Pa, and the treatment time is 10min. Add 10g / L sodium alginate solution afterwards and make the Cordyceps militaris spore concentration reach 10 9 pieces / ml, stirred evenly, freeze-dried at -30°C, 0.100mBar into 3d scaffolds;

[0036] Step 2: Soak the scaffold in 10g / L calcium chloride solution for 3 days, perform negative pressure flash explosion under 0.100mBar vacuum degree, and then carry out vacuum drying at 0°C and 0.100mBar to obtain the Cordyceps militaris fermented network scaffold;

[0037] Step 3: Cut the Cordyceps militaris fermentation network support into 1cm 3 Cubic packed column, the height-to-diameter ratio of the reaction column is 8:1, and the packed column volume is 50%. Prepare a conversion stock solution of 10g / L glucose, 0.05g / L potassium dihydro...

Embodiment 3

[0039] Step 1: activate the spores of Cordyceps militaris through plasma processor treatment, the conditions of plasma treatment are: the gas uses oxygen, the treatment power is 300W, the pressure is 60Pa, and the treatment time is 15min. Add 60g / L sodium alginate solution afterwards and make the Cordyceps militaris spore concentration reach 10 11 pieces / ml, stir evenly, freeze-dry at -20°C, 0.024mBar into 3d scaffolds;

[0040] Step 2: Soak the scaffold in 20g / L calcium chloride solution for 3 days, carry out negative pressure flash explosion under 0.024mBar vacuum degree, and then carry out vacuum drying at 4°C and 0.024mBar to obtain the Cordyceps militaris fermented network scaffold;

[0041] Step 3: Cut the Cordyceps militaris fermentation network support into 8cm 3 Cubic packed column, the height-to-diameter ratio of the reaction column is 10:1, and the packed column volume is 80%. Prepare a conversion stock solution of 15g / L glucose, 0.1g / L potassium dihydrogen phosphat...

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Abstract

The invention discloses a method for preparing cordycepin by fermenting cordyceps militaris through a calcium alginate freeze-dried network scaffold, and belongs to the field of biochemical engineering and food additives. The preparation method provided by the invention comprises the steps: (1) after cordyceps militaris spores are treated and activated through a plasma treater, a sodium alginate solution is added, even stirring is conducted, and the mixture is freeze-dried into a 3d scaffold; (2) the 3d scaffold is soaked through a calcium chloride solution, negative-pressure flash explosion is conducted, then vacuum drying is conducted, and the cordyceps militaris fermented network scaffold is prepared; and (3) columns are mounted on the cordyceps militaris fermented network scaffold andsoaked through a transformation original solution, and a cordycepin transformation solution is collected. The preparation method of the cordycepin is high in transformation efficiency, the preparationprocess can be continuously conducted, and industrialization promotion is facilitated.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a method for preparing cordycepin by fermenting Cordyceps militaris with a calcium alginate freeze-dried network support. Background technique [0002] Cordycepin is one of the main active ingredients in Cordyceps. Cordycepin has a variety of biological activities, such as anti-tumor, anti-proliferation, anti-metastasis, antibacterial, antiviral, immune regulation, and anti-inflammation. The preparation of cordycepin mainly includes chemical synthesis and biosynthesis. Due to the high production cost of chemically synthesized cordycepin, complex synthesis process, low yield and difficult product purification, cordycepin is mainly prepared by biosynthesis. There are two ways to prepare cordycepin by biosynthesis: one is to obtain the fruiting body of Cordyceps sinensis by solid fermentation, and then extract it. Due to the small porosity of the immobilized cell packing used i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/40C12N11/10C12R1/645
CPCC12P19/40C12N11/10
Inventor 汤佳鹏葛彦朱俐
Owner 广州大鱼创福科技有限公司
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