Tissue engineering bionic liver lobe structure based on 3D printing of living cells and preparation method thereof

A 3D printing and tissue engineering technology, applied in the field of biomedical engineering, can solve problems such as difficulty in achieving long-term survival of large-volume artificial tissues, difficulty in forming cell function integration, and support barriers
CN110403731AActive Publication Date: 2019-11-05THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
Publication Date
2019-11-05

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Abstract

The invention discloses a tissue engineering bionic liver lobe structure based on 3D printing of living cells and a preparation method thereof. Firstly, biological information of human body liver lobes is collected, and inputted into a computer for high biomimetic modeling; then, material separated printing and material-cell mixed printing output are performed with a hydrogel material and cells for constructing liver, bile duct epithelium, blood vessel endothelium, smooth muscle and fibroblast and the like required for the human liver lobes by a biological 3D printer, wherein a hollow part inblood vessels and bile ducts is printed by a casting agent; after all the hydrogel materials are solidified, the casting agent in the blood vessels and bile ducts are removed by means of enzymes or chelating reactions or temperature control or illumination and the like, and a vascular system and a bile duct system in the artificial bile duct-containing liver lobes are constructed. The circulationfeeding of the vascular system of the bionic liver lobes and gas exchange are achieved by connection of main blood vessels at two ends of the vascular system and a delivery pipe of a culture solution.The long-term survival and biological function of the bionic liver lobes in vitro and in vivo are achieved.
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Description

technical field

[0001] The invention belongs to biomedical engineering, and particularly relates to a tissue engineering bionic liver lobe structure based on living cell 3D printing and a preparation method, that is, to prepare an artificial vascularized bile duct that can survive for a long time and has biological functions by adopting the method of biological 3D printing liver lobe. Background technique

[0002] Existing artificial tissues are usually prepared by in vitro three-dimensional culture of cells, and only small-volume artificial tissues can be prepared. When the thickness of the artificial tissue exceeds 2 mm, it will cause deep tissue supply barriers, making it difficult for the artificial tissue to survive for a long time. In order to solve the problem of deep support barriers, researchers at home and abroad have carried out various explorations. For example, cells are seeded on porous scaffolds and soaked in medium for culture, so that nutrients can reach t...

Claims

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