A preparation method of self-beautifying biological material and self-beautifying biological material

Abstract

The present invention provides a method for preparing an autologous cosmetic biological material and provides an autologous cosmetic biological material, which is characterized in that it includes the following steps: collecting skin tissue; separating and culturing fibroblasts; preparing a blood platelet-rich plasma mixture preparation; autologous cosmetic biological The preparation of the material, the self-beauty biological material prepared by the method, has a short preparation time. High safety, high activity and good curative effect.

Description

technical field

[0001] The invention relates to a biological material and a preparation method thereof, in particular to a self-beautifying biological material and a preparation method thereof.

[0002] technical background

[0003] For plastic surgeons, facial soft tissue filling has always been a very challenging topic, and the development of injectable soft tissue materials is the difficulty of research. Since Wegener first reported the use of liquid silicone injection in 1957, the development of injectable soft tissue materials has gone through a long development process and has not been well resolved so far. The soft tissue filling materials used clinically are mainly synthetic-biological filling materials. It mainly includes liquid silica gel, silica gel particles, bovine collagen, fibrin, hyaluronic acid, etc. These biological materials have no biological activity and temporarily reside in the body, and there are different degrees of absorption problems and more or le...

Examples

Embodiment 1

[0041] This embodiment provides a method for making an autologous cosmetic biological material, comprising the following steps:

[0042] 1. Tissue collection: Under sterile conditions, use a skin sampler (skin trephine) to collect circular skin tissue with a diameter of 2-4mm behind the ear. Among them, the skin tissue behind the ear is a complete skin tissue, including epidermis, dermis, and subcutaneous tissue. Wash samples with phosphate buffered saline containing antibiotics and store in liquid nitrogen;

[0043] 2. Fibroblast isolation culture and primary (P0) cell culture:

[0044]Take out the tissue, wash it 1-2 times with DMEM (Dulbecco's Modified Eagle Medium) medium containing antibiotics after thawing, then use 75% alcohol to sterilize for a short time, and then wash it with phosphate buffer saline without calcium and magnesium ions. In this embodiment, epithelial cells and keratinocytes are removed by using a special culture medium for fibroblasts and the digesti...

Embodiment 2

[0074] This embodiment provides a method for making an autologous beauty biomaterial, the manufacturing steps of which are basically the same as those in Embodiment 1, the difference being that:

[0075] In fibroblast isolation culture and primary (P0) cell culture:

[0076] Take out the tissue, wash it 1-2 times with DMEM (Dulbecco's Modified Eagle Medium) medium containing antibiotics after thawing, then use 75% alcohol to sterilize for a short time, and then wash it with phosphate buffer saline without calcium and magnesium ions. Purify fibroblasts by removing epithelial cells and keratinocytes with fibroblast-specific culture medium and by limiting digestion time. Place the tissue in a composition containing collagenase and dispase for overnight digestion. After overnight digestion, remove the epidermis of the tissue block, then cut the tissue into pieces and move it to a special medium for culture for 20 days. When the degree of cell confluence reaches At 80%, the cells ...

Embodiment 3

[0095] This example provides a self-cosmetic biological material prepared by the method for making the self-cosmetic biomaterial described in Example 1. The self-cosmetic biomaterial is composed of fibroblasts, physiological saline, and plasma rich in autologous platelets. , cell growth factors, collagen.

[0096] The number of third-generation fibroblasts is 0.5*10 7 indivual.

[0097] The purity of the third passage fibroblasts was 99.7%.

[0098] The viability of the third passage fibroblasts was 99.5%.

[0099] The content of autologous plasma in 1ml of autologous beauty biomaterial is 0.8mL.

[0100] The content of physiological saline in 1ml of the self-cosmetic biological material is about 0.2mL.

[0101] The cell growth factors in the self-cosmetic biological material include fibroblast growth factor, epidermal growth factor, platelet-derived growth factor, transforming growth factor, vascular endothelial cell growth factor and insulin-like growth factor.

[0102]...

technical field

[0001] The invention relates to a biological material and a preparation method thereof, in particular to a self-beautifying biological material and a preparation method thereof.

[0002] technical background

[0003] For plastic surgeons, facial soft tissue filling has always been a very challenging topic, and the development of injectable soft tissue materials is the difficulty of research. Since Wegener first reported the use of liquid silicone injection in 1957, the development of injectable soft tissue materials has gone through a long development process and has not been well resolved so far. The soft tissue filling materials used clinically are mainly synthetic-biological filling materials. It mainly includes liquid silica gel, silica gel particles, bovine collagen, fibrin, hyaluronic acid, etc. These biological materials have no biological activity and temporarily reside in the body, and there are different degrees of absorption problems and more or le...

Examples

Embodiment 1

[0041] This embodiment provides a method for making an autologous cosmetic biological material, comprising the following steps:

[0042] 1. Tissue collection: Under sterile conditions, use a skin sampler (skin trephine) to collect circular skin tissue with a diameter of 2-4mm behind the ear. Among them, the skin tissue behind the ear is a complete skin tissue, including epidermis, dermis, and subcutaneous tissue. Wash samples with phosphate buffered saline containing antibiotics and store in liquid nitrogen;

[0043] 2. Fibroblast isolation culture and primary (P0) cell culture:

[0044]Take out the tissue, wash it 1-2 times with DMEM (Dulbecco's Modified Eagle Medium) medium containing antibiotics after thawing, then use 75% alcohol to sterilize for a short time, and then wash it with phosphate buffer saline without calcium and magnesium ions. In this embodiment, epithelial cells and keratinocytes are removed by using a special culture medium for fibroblasts and the digesti...

Embodiment 2

[0074] This embodiment provides a method for making an autologous beauty biomaterial, the manufacturing steps of which are basically the same as those in Embodiment 1, the difference being that:

[0075] In fibroblast isolation culture and primary (P0) cell culture:

[0076] Take out the tissue, wash it 1-2 times with DMEM (Dulbecco's Modified Eagle Medium) medium containing antibiotics after thawing, then use 75% alcohol to sterilize for a short time, and then wash it with phosphate buffer saline without calcium and magnesium ions. Purify fibroblasts by removing epithelial cells and keratinocytes with fibroblast-specific culture medium and by limiting digestion time. Place the tissue in a composition containing collagenase and dispase for overnight digestion. After overnight digestion, remove the epidermis of the tissue block, then cut the tissue into pieces and move it to a special medium for culture for 20 days. When the degree of cell confluence reaches At 80%, the cells ...

Embodiment 3

[0095] This example provides a self-cosmetic biological material prepared by the method for making the self-cosmetic biomaterial described in Example 1. The self-cosmetic biomaterial is composed of fibroblasts, physiological saline, and plasma rich in autologous platelets. , cell growth factors, collagen.

[0096] The number of third-generation fibroblasts is 0.5*10 7 indivual.

[0097] The purity of the third passage fibroblasts was 99.7%.

[0098] The viability of the third passage fibroblasts was 99.5%.

[0099] The content of autologous plasma in 1ml of autologous beauty biomaterial is 0.8mL.

[0100] The content of physiological saline in 1ml of the self-cosmetic biological material is about 0.2mL.

[0101] The cell growth factors in the self-cosmetic biological material include fibroblast growth factor, epidermal growth factor, platelet-derived growth factor, transforming growth factor, vascular endothelial cell growth factor and insulin-like growth factor.

[0102]...

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