Environment sensitive gel crosslinked with metalloprotein, and applications thereof

Abstract

The invention discloses a special large molecular gel with environment sensitivity. According to a preparation method, metalloprotein is embedded into a gel three dimensional network through chemicalcovalent bonds. The gel is prepared through copolymerization of an environment sensitive gel monomer or a skeleton formed through crosslinking of the environment sensitive gel monomer with a metalloprotein containing reaction groups or a complex of the metalloprotein with metal ions. Shrinkage and swelling of the environment sensitive gel with changing of environment can be realized, so that the chelating capacity of the metalloprotein on metal ions is adjusted, and high efficiency and selective capturing and release of metal ions are realized. The special large molecular gel with environmentsensitivity is capable of realizing high efficiency, stable, circulation capturing of metal ions at extremely concentration in liquid.

Description

technical field [0001] The invention belongs to the field of functional materials, and relates to an environment-sensitive gel of cross-linked metalloproteins and an application thereof. Utilize the properties of environment-sensitive gels that can shrink and swell with environmental changes to adjust the ability of metalloproteins and / or metal ion-binding domains of metalloproteins to chelate metal ions, so as to achieve efficient and selective capture of metal ions and freed. Background technique [0002] Heavy metal ion pollution in the environment will have serious impacts and harms on the human body and other organisms. These heavy metal ions are different from the pollution of other organic compounds. Most organic compounds can be purified or degraded by nature itself, while heavy metal ions can only be used as a One form is transformed into another form, which is easily enriched in living organisms, and then produces severe toxicity. For example, it will cause irrev...

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Problems solved by technology

For example, Xiaojie Ju et al. used NIPAM and BCAm to prepare hydrogels with temperature-sensitive properties, which can adsorb and desorb Pb using temperature changes. 2+ , but Pb 2+ The concentration is as high as 4mM, and cannot remove Pb at low concentrations (such as μM or nM levels) 2+ Ions (Ju XJ,Zhang SB,Zhou MY,Xie R,Chu LY,Novel heavy-metaladsorption materials:ion-recognition P(NIPAM-co-BCAm)hydrogels for removal oflead(II)ions.J Hazard Mater,2009,167 :114-118.); Neeshma Dave et al. used acrylamide and DNA-prepared hydrogels to detect and remove 10nM Hg 2+ , but the gel can only be regenerated by acid treatment (Dave N, Chan MY, Huang PJ, Smith BD, Liu JW, Regenerale DNAfunctionalized hydrogels for ultrasensitive instrument-free mercury(II) detection and removal in water.J AmChem Soc 2010,132 :12668-12673.); Aaron P.Esser-Kahn et al. use metallothionein as a gel formed by a cross-linking agent to remove heavy metal Cu 2+ , Zn 2+ , Cd 2+ and Hg 2+ However, the material can only be desorbed by chelating agent EDTA after adsorbing metals, and then re-adsorbed

In addition, this material has poor selectivity to metals, and the metal ion concentration used for adsorption is about 50ppb (Cd ion concentration is about 450nM), and the sensitivity is low (Esser-Kahn AP, Iavarone AT, Francis MB, Metallothionein-cross-linked hydrogels for the selective removal of heavy metals from water. J Am Chem Soc, 2008, 130:15820-15822.)

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