Detection of targets using magnetic resonance

A magnetic resonance and nuclear magnetic resonance technology is applied in the directions of magnetic resonance measurement, analysis by nuclear magnetic resonance, and measurement of magnetic variables. It can solve the problems of unreliable measurement results, increase the cost and size of the DMR system, and achieve relief of huge burdens. The effect of accurate diagnosis

Pending Publication Date: 2019-11-12
THE GENERAL HOSPITAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, measurements obtained in uncontrolled environments may be unreliable
Additionally, efforts to control the temperature of the environment and system can increase the cost and size of DMR systems to the point where they are not ideal devices for point-of-care use

Method used

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  • Detection of targets using magnetic resonance
  • Detection of targets using magnetic resonance
  • Detection of targets using magnetic resonance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] Example 1: Evaluation of DMR temperature change compensation

[0128] The ability of the DMR system to compensate temperature changes was evaluated through experiments. Test samples were prepared by adding magnetic nanoparticles (cross-linked iron oxide particles aminated to provide primary amine ligands (CLIO-NH2)) in phosphate buffered saline (PBS). It should be noted that the surface of the CLIO particles of this ferrite core (approximately 7 nm in diameter) was covered with a layer of 10 kDa dextran, which was cross-linked with epichlorohydrin to stabilize the coating, and subsequently aminated to provide primary amine ligands (CLIO-NH 2 ). The hydraulic diameter of CLIO as determined by dynamic light scattering (Zetasizer 1000HS laser particle sizer, Malvern Instruments) of the samples thus prepared was 38 nm.

[0129] Samples are added to sample tubes and loaded onto the DMR system. We used the following CPMG pulse sequence to measure T 2 : For short T 2 (...

Embodiment 2

[0133] Example 2: DMR System Benchmarking

[0134] To verify the accuracy of the system, the performance of the new DMR system was compared with another large benchtop NMR relaxation instrument. A benchtop NMR relaxation instrument (minispec mq20 from Bruker) was run at an external magnetic field of 0.47T under similar conditions to the new DMR system. As discussed in Example 1, we prepared samples containing different amounts of CLIO. Using the same pulse sequence parameters described in Example 1 above, the longitudinal T of the magnetic nanoparticles was measured using reverse recovery and CPMG pulse sequences, respectively. 1 and the transverse relaxation time T 2 . By recording the relaxation time, the longitudinal r is calculated 1 and r 2 Relaxation rate, where the relaxation rate is magnetic particle-induced T 1 and T 2 The capacity to change is not equal to the reciprocal of the corresponding relaxation time. Figure 10 with Figure 11 are graphs showing th...

Embodiment 3

[0136] Example 3: Avidin detection

[0137] To demonstrate the biological application of the new DMR system for the rapid and sensitive diagnosis of small molecule disease markers, we validated the avidin interaction. We used biotinylated CLIO (CLIO-biotin) to detect avidin. To demonstrate CLIO targeting specificity, we modified the particle surface with an affinity ligand. To make CLIO-NH 2 For biotinylation, we dissolved 2 mg of sulfosuccinimidyl-6-(nicotinamide) hexanoate (sulfo-NHS-LC-biotin) with 0.5 mg of CLIO-NH 2 Mix and keep at room temperature for 3 hours, then use membrane filtration (Amicon with a molecular weight of 30,000, Millipore Company), and then use Sephadex G-50 (GE Healthcare Company, with PBS (pH 7.2) as the eluent buffer ) to purify biotinylated CLIO. Concentration between 800nM [A V ] to 2nM [A V ] The dilution solution is to utilize stock solution concentration to be 1 mg / ml [A V ] PBS solution (ImmunoPure avidin, Pierce Biotechnology Compan...

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Abstract

A portable magnetic resonance system includes a permanent magnet, a nuclear magnetic resonance probe, and control electronics. The control electronics are configured to transmit to the probe a magnetic resonance excitation signal having an excitation frequency f, receive from the probe a magnetic resonance measurement signal, detect in the magnetic resonance measurement signal a magnetic resonancefrequency f0, and automatically adjust the excitation frequency f until the difference between the excitation frequency and the magnetic resonance frequency is approximately equal to a target offset.

Description

[0001] priority claim [0002] This application asserts U.S. Provisional Patent Application No. 61 / 466,135, filed March 22, 2011, U.S. Provisional Patent Application No. 61 / 515,065, filed August 4, 2011, and The entire disclosure of which is hereby incorporated by reference in its entirety for the benefit of and priority of U.S. Provisional Patent Application No. 61 / 515,150. [0003] Statement on Federally Funded Research Outcomes [0004] Research work described herein was supported by funding provided through National Institutes of Health grant numbers R01-EB004626, HHSN268201000044C, U54-CA119349, and T32-CA79443, each of which is administered by the federal government, which has certain rights in this invention . [0005] Background of the invention [0006] The rapid and accurate measurement of biomarkers in biological samples provides various information for the quantification of chemical entities in order to facilitate the early detection of diseases and provide insigh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08G01R33/465G01R33/31G01R33/44
CPCG01N33/57484G01N2333/4725G01N2333/485G01N2333/70596G01N2333/71G01N2800/00G01N2800/52G01N24/088G01N33/574G01N21/6428G01N33/54326G01N33/57492G01N33/587G01R33/1269
Inventor R.韦斯莱德李学虎D.伊萨多尔
Owner THE GENERAL HOSPITAL CORP
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