Glow-type chemiluminescence sensor constructed based on hydrogel and preparation method and application thereof
A chemiluminescence and hydrogel technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials react chemically, can solve the problems of limited application, complex electrode modification process equipment and other problems, and achieve enhanced chemiluminescence intensity , long chemiluminescent time and short detection time
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[0049] Example 1 ABEI / Co 2+ / CS synthesis of hydrogel
[0050] In 15mL alkaline solution (the mass ratio is LiOH: KOH: Urea: H 2 O=4.5:7:8:80.5) stir and disperse 0.4g chitosan powder, add 0.6mL CoCl 2 (1mM) solution, stir for 5 minutes, and refrigerate at -20°C until completely frozen. After thawing at room temperature, add 1.5mL ABEI (10mM) solution and stir for 5 hours to evenly disperse in the system to obtain ABEI / Co 2+ / CS hydrogel and stored at 4°C.
[0051] figure 2 A shows the synthesized ABEI / Co 2+ SEM image of / CS hydrogel; the characteristic absorption peak of ABEI is located at 291nm of the red line. The UV-visible absorption spectrum proves that ABEI is successfully encapsulated in the hydrogel.
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[0052] Example 2 ABEI / Co 2+ / CS synthesis of hydrogel
[0053] In 15mL alkaline solution (the mass ratio is LiOH: KOH: Urea: H 2 O=4.5:7:8:80.5) stir and disperse 0.4g chitosan powder, add 0.6mL CoCl 2 (0.1mM) solution, stir for 5 minutes, and refrigerate at -20°C until completely frozen. After thawing at room temperature, add 1.5mL ABEI (4mM) solution and stir for 5 hours to evenly disperse in the system to obtain ABEI / Co 2+ / CS hydrogel and stored at 4°C.
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[0054] Example 3 Synthesis of MOF-Pt
[0055] First, weigh 0.2g TCPP and 0.12g AlCl 3 ·3H 2 O and 0.144 g of CTAB were dissolved in 20 mL of water, stirred for 10 minutes and placed in a 20 mL reactor, and then reacted at 180°C for 16 hours. After centrifugation at 3000 rpm, the supernatant was centrifuged at 13000 rpm to obtain a precipitate. The precipitate was washed twice with DMF, water and acetone, respectively, and then dried in vacuum overnight. Next, the purple and black precipitates were obtained by vacuum drying at 150°C for 7 hours for further reaction. Then weigh out 0.038g of precipitate and 0.064g of Cu(Ac) 2 Dissolved in 8ml DMF organic solvent, stirred for 10 minutes, then poured into a 20ml reactor and reacted at 100°C for 24 hours to obtain a red-black blocky precipitate. After centrifugation at 13000 rpm, the precipitate was washed twice with DMF, water and acetone, and then dried in vacuum overnight to obtain product 1.
[0056] Finally, add 0.5ml of H 2 PtCl...
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