Photoelectrochemical sensor for detecting kanamycin and its preparation method and application
A technology of kanamycin and photoelectrochemistry, applied in the field of detection and analysis, can solve the problems of lack of photothermal stability, limit the activity of biosensors, hinder the detection of kanamycin, etc., and achieve rapid detection, good stability and high sensitivity Effect
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Embodiment 1
[0039] A method for preparing a photoelectrochemical sensor for detecting kanamycin, comprising the following steps:
[0040] (1) Synthesis of octahedral Cu 2 O precursor; pipette 5mL0.1mol / L pyridine solution and add dropwise to 5mL0.05mol / L CuSO 4 ·5H 2 In the O solution, blue pyridinesulfur copper colloids are produced after adding pyridine; the mixed solution is stirred for 20min, and 10mL0.1mol / LNaOH is added to adjust the mixed solution to alkalinity, ensuring that 5mL0.1mol / L glucose solution is added under alkaline conditions. Under the condition of 75°C in a water bath, heat the above reaction mixture for 10min, Cu 2+ Reduction by glucose affords octahedral Cu 2 O, centrifuged, washed, vacuum dried. The resulting octahedral Cu 2 The scanning electron microscope image of O is shown in figure 1 , it can be seen that the method successfully obtained octahedral Cu 2 O.
[0041] (2) Preparation of spherical core-shell structure CuO@Pd;
[0042] Weigh 0.01gCu 2 O ...
Embodiment 2
[0053] A method for preparing a photoelectrochemical sensor for detecting kanamycin, comprising the following steps:
[0054] (1) Synthesis of octahedral Cu 2 O precursor; pipette 5mL0.1mol / L pyridine solution and add dropwise to 5mL0.05mol / L CuSO 4 ·5H 2 In the O solution, blue pyridinesulfur copper colloids are produced after adding pyridine; the mixed solution is stirred for 50min, and 10mL0.1mol / L NaOH is added to adjust the mixed solution to alkaline, and 5mL 0.1mol / L glucose solution is added under alkaline conditions, In a water bath at 85°C, heat the above reaction mixture for 3 min, Cu 2+ Reduction by glucose affords octahedral Cu 2 O, centrifugal, washing, vacuum drying;
[0055] (2) Preparation of spherical core-shell structure CuO@Pd;
[0056] Weigh 0.05gCu 2 O precursor dispersed in 1ml distilled water to obtain Cu 2 O suspension, then Cu 2 O suspension was slowly dropped into 1mL 0.05mol / L PdCl 2 solution to obtain a mixture; then the mixture was placed ...
Embodiment 3
[0067] The photoelectrochemical sensor prepared by embodiment 1 is applied to the detection of kanamycin, specifically:
[0068] (1) Use the Tianjin Aida LED light source and the electrochemical workstation (CHI832D) to automatically control the three-electrode system for testing, the platinum column electrode is the counter electrode, and the Ag / AgCl is the reference electrode. The sensor is the working electrode, tested in TE buffer;
[0069] (2) Test the standard solution of kanamycin with different concentrations by using the time current method I~t as the test technology. The concentrations of the standard solution of kanamycin are respectively a: 0, b: 0.01nM, c: 0.05nM, d : 0.1nM, e: 50nM, f: 100nM, g: 150nM, h: 200nM, i: 250nM, j: 300nM, k: 350nM, l: 400nM, m: 450nM, n: 500nM, o: 600nM, p: 800nM), set the voltage to 0V, and perform photoelectric signal detection under the irradiation of a blue light source with a wavelength of 465nm;
[0070] (3) The photocurrent res...
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