A method for spraying Robinia pseudoacacia on exposed rock walls of shale with high efficiency and rapid regreening and improving soil pH

A technology for improving soil and rock walls, applied in the direction of soil preparation methods, soil conditioning materials, chemical instruments and methods, etc., can solve the problems of soil drought resistance decline, root system reduction, soil water loss, etc., to improve the content of organic matter, pH Value improvement, the effect of reducing the amount of application

A technology for improving soil and rock walls, applied in the direction of soil preparation methods, soil conditioning materials, chemical instruments and methods, etc., can solve the problems of soil drought resistance decline, root system reduction, soil water loss, etc., to improve the content of organic matter, pH Value improvement, the effect of reducing the amount of application

CN110521323BActive Publication Date: 2022-02-01NANJING FORESTRY UNIV
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  • A method for spraying Robinia pseudoacacia on exposed rock walls of shale with high efficiency and rapid regreening and improving soil pH
  • A method for spraying Robinia pseudoacacia on exposed rock walls of shale with high efficiency and rapid regreening and improving soil pH

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Acquisition and identification of strains

[0032] Soil samples were collected from 10 cm of rhizosphere soil on both sides of Yueyang Avenue, Yueyang City, using the dilution coating plate method, in a 35 ° C incubator, with nutrient agar solid medium (NA medium: 10 g of peptone; 3 g of beef powder; Sodium chloride 5g; Agar 15g; Sterile water 1000mL; pH value 7) Cultivate for 2-3 days, pick different colonies by visual observation, and repeatedly streak and purify to obtain several single colonies of different species.

[0033] Select a single colony to make a plate, and send it to Shanghai Jinyu Medical Laboratory for sequencing to obtain the 16S rDNA gene sequence, as shown in SEQID NO.1. The measured 16S rDNA gene sequence was compared with the sequence in the GenBank database by BLAST. The results showed that the similarity between the strain and Kocuria polaris was 99.32%. Combined with morphological characteristics (yellow in the center, light yellow at the ...

Embodiment 2

[0041] 1. Preparation of microbial mixture

[0042] 1) Take Koukia X-22, Microbacterium X-26, Bacillus X-28 strains, and Microbacterium X-18, respectively, on nutrient agar solid medium, activate at 35°C for 24 hours;

[0043] 2) Pick a ring of sludge from the activated Microbacterium X-26, Bacillus X-28 and Microbacterium X-18 with an inoculation loop and add them to the LB liquid medium respectively. 22 into the NA liquid medium, 35 ° C, 200r / min constant temperature shaking for 24 hours to prepare the seed liquid; the pH value of the seed liquid changes are shown in Table 2;

[0044] Table 2 pH change results before and after seed solution

[0045] pH before culture pH after incubation culture medium Microbacterium sp. X-18 6.8-7.0 4.78 LB Koukia X-22 6.8-7.0 8.35 NA Microbacterium sp. X-26 6.8-7.0 8.77 LB Bacillus X-28 6.8-7.0 8.39 LB

[0046] 3) Take the seed liquid according to the inoculation amount of 3% and ino...

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Abstract

The invention discloses a method for spraying black locust on exposed rock walls of shale for efficient and rapid regreening and improving soil pH, and belongs to the technical fields of ecological restoration and soil improvement. The guest soil spraying method is used to spray mixed microorganisms, organic fertilizers, and soil on the exposed shale rock wall, and the greening plant is black locust, so as to achieve efficient and rapid regreening and improve soil pH. The mixed microorganisms are composed of Koukia X-22, Microbacterium X-26, Bacillus X-28, and Microbacterium X-18, and the mixed microorganisms are added to organic fertilizer and soil as fermentation broth. The weight ratio of the mixed microorganisms, organic fertilizer and soil is 1:1:8. This method realizes the synergy between microbial flora and leguminous nitrogen-fixing bacteria, promotes the rapid growth of Robinia pseudoacacia on the exposed shale rock wall, can significantly increase the organic matter content, available phosphorus content, and pH value of Robinia pseudoacacia soil, improve soil fertility, and reduce the application of chemical fertilizers. It can provide a low-cost, high-efficiency new method for efficient and rapid regreening of shale exposed rock walls.

Description

technical field [0001] The invention belongs to the technical field of soil improvement, and in particular relates to a method for spraying black locust on exposed rock walls of shale for efficient and rapid regreening and improving soil pH. Background technique [0002] Soil is the foundation of forestry production, an important carrier of nutrients in material cycle and energy flow, and one of the important factors affecting the environment. With the advancement of human civilization, a series of activities such as mining and road construction have caused ecological problems such as the destruction of mountain vegetation, exposed rock walls, and increasing soil erosion. Human development and utilization of land will not only change the soil structure, but also change the soil fertility and activity, etc., which has a serious impact on the cycle of soil materials. [0003] Soil acidity and alkalinity are important indicators for evaluating soil nutrients, and have extremel...

Claims

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Application Information

Patent Timeline
01 Feb 2022
Publication
CN110521323B
IPC
A01B79/02; C09K17/40; C09K109/00; C09K105/00; C09K101/00
CPC
A01B79/02; C09K17/40; C09K2109/00; C09K2101/00; C09K2105/00; C05F11/10; A01G24/60; C05F5/008
Inventors
庄家尧; 刘超