Construction method of living cell probe based on neutrophilic granulocyte

A neutrophil and construction method technology, applied in the field of neutrophil-based living cell probe construction, can solve the problem of affecting neutrophil activity and biological characteristics, affecting high T1 relaxation rate, and tumor delivery ability Damage and other problems, to achieve the effect of simple nano-construction method, little impact on cell physiological functions, and easy access
CN110585449AInactive Publication Date: 2019-12-20NANTONG TUMOR HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANTONG TUMOR HOSPITAL
Publication Date
2019-12-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of biomedical science detection, and discloses a construction method of a living cell probe based on neutrophilic granulocyte. The construction method comprises the steps of: A, performing BSA reduction; B, synthesizing Gd loaded BSA nanoparticles; C, labeling the Gd loaded BSA nanoparticles with Bodipy; and D, constructing a neutrophilic granulocyte probe: firstly with PBS as a medium, adding DTNB to a product solution in the step C, performing interaction at room temperature for a certain time to finish activation of BSA nanoparticles, after ultrafiltration and purification are performed, diluting the activated BSA nanoparticles to 0.1mg mL<-1> with an RPMI 1640 culture medium without FBS, then performing incubation with 1.25*10<6>cells.mL<-1>of neutrophilic granulocyte at room temperature, and then performing washing with ice-cold PBS so as to obtain the neutrophilic granulocyte probe.
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Description

technical field

[0001] The invention relates to the technical field of biomedical detection, and relates to the construction of live cell probes, in particular to a neutrophil-based live cell probe construction method. Background technique

[0002] Timely and accurate identification of tumor lesions is an important prerequisite for clinical cancer intervention and the goal of tumor imaging diagnosis. Advances in nanoparticle-based molecular imaging have greatly improved the sensitivity and specificity of tumor detection. For example, inspired by biomineralization, previous studies successfully constructed chelated gadolinium ions (Gd 3+ ) bovine serum albumin (BSA) nanoparticles, which have obvious advantages over commercial gadolinium contrast agent (DTPA) in magnetic resonance imaging (MRI) detection of tumors. Some reports have made further studies on Gd-loaded nanoparticles, especially modifying the ligands of overexpressed cancer cell receptors to endow them with the ...

Claims

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