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Application of chlorella sorokiniana in functional feed for improving health condition of fishes

A health status, chlorella technology, applied in application, animal feed, animal feed, etc., can solve the problems of drug-resistant bacteria, environmental pollution, etc., and achieve the effects of promoting growth, avoiding ecological environment pollution, and reducing the incidence of diseases

Inactive Publication Date: 2019-12-24
DEMETER BIOTECH (ZHUHAI) LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the face of high incidence of diseases, the use of antibiotics is the most common coping strategy for aquaculture practitioners; however, although the use of antibiotics promotes the growth of fish and reduces the occurrence of diseases, it also brings various problems, such as resistance Bacteria production, environmental pollution and food safety [3-5]

Method used

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  • Application of chlorella sorokiniana in functional feed for improving health condition of fishes
  • Application of chlorella sorokiniana in functional feed for improving health condition of fishes
  • Application of chlorella sorokiniana in functional feed for improving health condition of fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Cultivation of Sorokin Chlorella starch-deficient mutant strain GT-1-SLM2 (preservation number CGMCC No.13862)

[0032] The medium formula for heterotrophic culture is as follows:

[0033] In basal culture medium and supplementary culture medium: glucose 20g / L; KNO 3 2g / L; KH 2 PO 4 1.2 g / L; MgSO 4 ·7H 2 O 1.2g / L; Trisodium Citrate 0.2g / L; CaCl 2 2H 2 O mother liquor (1000×) 1ml; FeSO 4 ·7H 2 O and EDTA·2Na mother solution (1000×) 1mL; trace element mother solution (1000×) 1mL;

[0034] CaCl 2 Preparation of mother liquor: 105g CaCl 2 2H 2 O dissolved in 1000ml H 2 O;

[0035] FeSO 4 ·7H 2 O and Na 2 Preparation of EDTA mother liquor: 16g FeSO 4 ·7H 2 O and 2.1 g Na 2 EDTA dissolved in 1000ml H 2 O;

[0036] Composition of trace element mother liquor: H 3 BO 3 2.86g / L, ZnSO 4 ·7H 2 O 0.222g / L, MnCl 2 4H 2 O1.81g / L, NaMoO 4 0.021g / L, CuSO 4 2H 2 O 0.07g / L. Adjust the pH to 6.5.

[0037] The cultivation method is:

[0038] Primary cul...

Embodiment 2

[0069] Embodiment 2 is to reduce the addition amount of sorokin chlorella starch-deficient mutant strain algae flour from 5% to 2% in the experimental group feed of embodiment 1, and adopt fishmeal and soybean oil to adjust the feed of the control group, so that The diets of the experimental group and the control group conformed to the principle of equal nitrogen and equal fat.

[0070] The results of the experiment found that after adding 2% algae powder of the starch-deficient mutant strain of Chlorella sorokinus as an additive to the feed, although the fish's food intake, growth and weight gain speed, antioxidant enzymes in the body, and fish immunity were improved to a certain extent Or enhance the improvement effect, but the improvement effect is not as obvious as Example 1.

Embodiment 3

[0072] The formulations of the experimental group feed and the control group feed in Example 1 were used for culturing carp. Specifically, two carp are raised in two large tanks with oxygen supply and temperature control respectively. During the breeding period, the average water temperature of the fish tank was kept at 9.33°C, the dissolved oxygen was 8.83mg / L, the natural pH of the normal water quality, the total ammonia nitrogen was 0.05mg / L, and under the condition of natural light, a 90-day breeding experiment was carried out to explore the starch of Chlorella sorokini Application effect of defective mutant strain algae powder in carp breeding.

[0073] Experimental result finds, the fish body weight of experimental group feed feeding increases to 400g by initial 150g. At the same time, the feed quality of the experimental group was stable, and the feed coefficient was around 0.85. The feeding rate and weight gain rate of carp fed with the experimental group feed increa...

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Abstract

The invention relates to an application of chlorella sorokiniana in a functional feed for improving the health condition of fishes. The Chlorella sorokiniana disclosed by the invention is a starch defect type mutant strain GT-1-SLM2, and a preservation number of the strain is CGMCC No.13862, and the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC, an address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing). According to the invention, chlorella sorokiniana is used as starch-deficient mutant strain algae powder and is added into the fish feed, which can replace part of fish meal, can significantly improve the food intake, growth weight gain speed, oxidation resistance and immunity of fishes, the product can replace traditional antibiotics, and avoids the problems of ecological environment damage and food safety caused by large-scale application of antibiotics in the feed. Therefore, the invention provides a brand-new, green and safe immunopotentiator, a phagostimulant or an antioxidant.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to the application of sorokin chlorella in functional feed for improving the health of fish. Background technique [0002] According to the latest statistics from FAO, in 2016, the world's aquaculture animal production was 80 million tons, and China's reached 49.51 million tons, accounting for 62% of the world's farmed fish production. [1] . Aquaculture is one of the fastest growing agricultural sectors. However, behind its rapid development is the intensification of aquaculture, the deterioration of water quality, and the high incidence of diseases. [2] . According to statistics, the annual loss of aquaculture animals in my country due to diseases is as high as 10-20 billion yuan. [2,3] . In the face of high incidence of diseases, the use of antibiotics is the most common coping strategy for aquaculture practitioners; however, although the use of antibiotics promotes the gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/16
CPCA23K10/16A23K50/80Y02A40/818
Inventor 胡强陈卫军韩丹翔
Owner DEMETER BIOTECH (ZHUHAI) LTD
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