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Polypeptide RLY4 and uses thereof for promoting liver regeneration and inhibiting hepatocyte apoptosis

A liver cell and liver regeneration technology, applied in the field of cell proliferation and apoptosis, can solve the problems of RLY4 regeneration and inhibition of liver cell apoptosis, and achieve the effect of promoting liver cell proliferation, small molecular weight, and low immunogenicity

Active Publication Date: 2019-12-31
LINK HEALTH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] RLY4 is a polypeptide containing 4 amino acids (RLYE). It has been reported that RLY4 can inhibit angiogenesis induced by vascular epidermal growth factor (VEGF), and can be used for the treatment of diseases such as tumors and wet macular degeneration (see Chinese patent CN108350029A, and non- Patent Documents 1, 2, 3), but there is no report that RLY4 is used to promote liver cell regeneration and inhibit liver cell apoptosis

Method used

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  • Polypeptide RLY4 and uses thereof for promoting liver regeneration and inhibiting hepatocyte apoptosis
  • Polypeptide RLY4 and uses thereof for promoting liver regeneration and inhibiting hepatocyte apoptosis
  • Polypeptide RLY4 and uses thereof for promoting liver regeneration and inhibiting hepatocyte apoptosis

Examples

Experimental program
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Effect test

Embodiment 2

[0042] Example 2 Detecting the Activity of Promoting Hepatocyte Proliferation in Vitro

[0043] In this example, the human liver cell line LO2 was purchased from ATCC.

[0044] In this example, the method for detecting the activity of the polypeptide RLY4 described in Example 1 in promoting the proliferation of liver cells in vitro is as follows:

[0045] After LO2 cells were revived according to conventional methods, they were placed at 37°C and 5% CO 2 In the incubator, culture with DMEM complete medium containing 10% FBS and 1% double antibody. When the cells have grown to about 85% of the bottom of the bottle, digest with trypsin-EDTA, add DMEM complete medium and blow gently to make a cell suspension, adjust the cell concentration, inoculate into a 96-well plate, 100 μL per well, Then place at 37°C, 5% CO 2 cultured in an incubator until use.

[0046] Inoculate LO2 in the logarithmic phase at a density of 5000 / well in a 96-well plate and culture for 24 hours. Divided...

Embodiment 3

[0048] Example 3 Detecting the Activity of Inhibiting Hepatocyte Apoptosis in Vitro

[0049] In this example, the method for detecting the activity of the polypeptide RLY4 described in Example 1 in inhibiting liver cell apoptosis in vitro is as follows:

[0050] Human hepatocytes HL-7702 were spread in 96-well plates, and the cell concentration was 1×10 5 cells / ml, 100 μl per well, that is, 1×10 cells per well 4 indivual.

[0051] Each well was treated with 20ng / mL actinomycin D2 (Act D2) for 30min and 80ng / ml tumor necrosis factor α (TNF-α) for 48h to establish the apoptosis model.

[0052] The corresponding polypeptide RLY4 was added for treatment, the dosing volume was 10 μl, and the final concentrations were 0.1, 1, and 10 μg / ml respectively, and the same volume of PBS was used as a negative control, and 0.2 μg / ml epidermal growth factor (EGF) was used as a positive control.

[0053] After 24 hours of drug treatment, add 100 μl to each well Reagent ( 3 / 7 provided by...

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Abstract

The present invention discloses a polypeptide and uses thereof for promoting liver cell proliferation and / or inhibiting hepatocyte apoptosis. An amino acid sequence of the polypeptide capable of promoting hepatocyte proliferation and / or inhibiting hepatocyte apoptosis has a sequence identity of 3 / 4 or more compared with an amino acid sequence shown in SEQ ID NO:1, length of the polypeptide is 4 amino acids, and the polypeptide has advantages of small molecular weight, easy synthesis, low immunogenicity, etc., and thus has broad application prospects in treatment of liver diseases.

Description

technical field [0001] The invention relates to the technical field of cell proliferation and apoptosis, in particular to a polypeptide RLY4 and its use for promoting liver cell proliferation and / or inhibiting liver cell apoptosis. Background technique [0002] The liver is the largest internal organ in the human body (accounting for about 2.5% of the total body mass), and it is also the central metabolic organ. Parenchymal cells are mainly responsible for liver function, accounting for 80% of the total liver cells. Hepatocytes play a series of roles, including producing plasma, synthesizing carrier proteins, detoxifying digests, conjugating and secreting hormones, regulating the synthesis and metabolism of blood lipids and amino acids, etc. [0003] Apoptosis is an active suicide process of cells under specific circumstances. Apoptosis is two different patterns of apoptosis and death observed by Kerr et al. in 1972 when studying liver ischemia. Apoptosis is characterized...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/11A61K38/07A61P1/16
CPCC07K5/1019A61P1/16A61K38/00
Inventor 张时群卢肖宇王华李天然
Owner LINK HEALTH GRP
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