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Rapid Determination Method of Pyridoxal Phosphate Using Nanogold as Chromogenic Probe

A technology for rapid determination of pyridoxal phosphate, applied in analytical chemistry and nanometer fields, can solve problems such as limited application, and achieve the effects of fast detection speed, simple and fast preparation process, and high detection sensitivity

Active Publication Date: 2021-10-15
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although most of the above techniques allow sensitive and selective detection of pyridoxal phosphate, the need for sophisticated instruments and trained operators limits their use in routine testing

Method used

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  • Rapid Determination Method of Pyridoxal Phosphate Using Nanogold as Chromogenic Probe
  • Rapid Determination Method of Pyridoxal Phosphate Using Nanogold as Chromogenic Probe
  • Rapid Determination Method of Pyridoxal Phosphate Using Nanogold as Chromogenic Probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of mercaptoethylamine-gold nanoparticles: First, take 400 μL of mercaptoethylamine hydrochloride with a concentration of 213 mmol / L and 2.23 mL of HAuCl with a concentration of 10 mg / mL 4 Add it to a beaker containing 37.5 mL of water, and stir at room temperature for 20 min. After the end, add 10 μL of NaBH with a concentration of 10 mmol / L to the above reaction solution 4 , and then continue to stir at room temperature for 30 minutes to obtain the crude mercaptoethylamine-nanogold. Purify the obtained crude mercaptoethylamine-gold nanoparticles with a dialysis bag with a molecular weight cut-off of 7000 to obtain pure mercaptoethylamine-gold nanoparticles. The obtained mercaptoethylamine-nanogold pure product was sealed and protected from light, and stored in a refrigerator at 4 °C for future use. All glassware used in the above process was soaked in aqua regia, washed thoroughly with double distilled water, and dried.

Embodiment 2

[0035] Add 0.2 mL of pyridoxal phosphate with a final concentration of 0.5 μmol / L and 0.2 mL of mercaptoethylamine-nanogold prepared in Example 1 to 0.6 mL of acetate buffer solution (pH = 6.0, 10 mmol / L) in sequence, Mix well and react at room temperature for 1 minute. The color of mercaptoethylamine-gold nanoparticles changed from wine red to blue (see figure 1, in the figure A: mercaptoethylamine-gold nanoparticles, B: mercaptoethylamine-gold nanoparticles + pyridoxal phosphate), and a new absorption peak is generated at a wavelength of 700 nm (see figure 2 , in the figure A: mercaptoethylamine-gold nanoparticles, B: mercaptoethylamine-gold nanoparticles + pyridoxal phosphate).

Embodiment 3

[0037] Add 0.2 mL of pyridoxal phosphate with a final concentration of 0.5 μmol / L and 0.2 mL of mercaptoethylamine-nanogold prepared in Example 1 to 0.6 mL of acetate buffer solution (pH = 6.0, 10 mmol / L) in sequence, Mix well, react at room temperature for 1 minute, and then carry out transmission electron microscope test. Depend on image 3 It can be seen that when not reacting with pyridoxal phosphate, mercaptoethylamine-gold nanoparticles are spherical and well dispersed (A in the figure); after reacting with pyridoxal phosphate, mercaptoethylamine-gold nanoparticles are obviously aggregated ( Figure B).

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Abstract

The invention discloses a rapid determination method of pyridoxal phosphate using nano-gold as a color probe, which is characterized in that the interaction between pyridoxal phosphate and nano-gold leads to the aggregation of nano-gold to show solution color and ultraviolet-visible absorption spectrum characteristics To determine the concentration of pyridoxal phosphate. When visually observing the color characteristics, with the gradual increase of pyridoxal phosphate concentration (0~0.5μmol / L), the color of mercaptoethylamine-gold nanoparticles changes from wine red→pink→purple→blue; When determining the absorbance ratio A 700 / A 525 , when the concentration of pyridoxal phosphate changes in the range of 0.25~0.5μmol / L, the absorbance ratio A of mercaptoethylamine-nano gold 700 / A 525 Increased with increasing pyridoxal phosphate concentration.

Description

technical field [0001] The invention relates to a rapid assay method for pyridoxal phosphate content using nano-gold as a color probe, and belongs to the fields of analytical chemistry and nanotechnology. Background technique [0002] Gold nanoparticles have attracted extensive attention due to their facile preparation and biofunctionalization, excellent biological stability, and unique spectral properties. The surface plasmon absorption bands of gold nanoparticles lie in the visible region of the electromagnetic spectrum and are influenced by the morphology of the nanoaggregates. Typical colloidal gold nanoparticles are wine red, while their aggregates appear purple or blue, which is due to the shift of the surface plasmon absorption band of gold nanoparticles to long wavelengths. Methods based on this principle can be used for the detection of various analytes such as cells, proteins, DNA, and metal ions. [0003] Pyridoxal phosphate, also known as -5'-pyridoxal phosphat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/3151G01N21/78G01N2021/3155
Inventor 邓豪华陈伟黄开源彭花萍何少斌
Owner FUJIAN MEDICAL UNIV
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