Improved grease composition

[0021] Example 1

[0022] Put a total of 200 g of commercially available CLA and caprylic acid glyceride (CLA: caprylic acid glyceride = 3, mol) into a 500ml flask with stopper, preheat it to 50°C and add immobilized lipase (Novo435, NovozymesA) /S) 40g, placed in a constant temperature water bath shaker at 50℃, oscillated at 200r/min for 5h, and then stopped the reaction. After the product was filtered to remove the immobilized enzyme particles, the fatty acid in the product was further separated by molecular distillation. The heavy fraction was then decolorized and deodorized in a conventional manner to obtain an oil and fat composition 1.

[0023] Under the conditions of 50% water addition and vigorous stirring, use the free 1,3-position specific lipase as a catalyst for the commercially available rapeseed oil (LipAse F-AP15, product of AmAno Enzyme Inc., or high hydrolysis from other companies). Enzyme-active free lipase product) at 40°C for the hydrolysis reaction of oil and fat, and stop the reaction after reaching a certain degree of hydrolysis. After incubation at 65°C for 0.5h, the enzyme is inactivated and left to stand to remove most of the water. In addition to the remaining water, add diatomaceous earth, stir for 0.5h, and filter to remove the remaining enzyme protein. The remaining product is further separated by molecular distillation, and then decolorized and deodorized in a conventional manner to obtain diglycerides and triglycerides. The oil and fat composition 2 and free fatty acid-based composition 3.

[0024] The oil and fat composition 1 and the oil and fat composition 2 were uniformly mixed in a volume ratio of 20:80 to obtain a fat and oil composition 4.

[0025] Example 2

[0026] Put a total of 500 g of commercially available CLA ethyl ester and caprylic acid glyceride (CLA ethyl ester: caprylic acid glyceride = 3, mol) into a stirred 60°C constant temperature reactor and mix uniformly. The immobilized lipase (Lipozyme TL IM, Novozymes A/S) 10g is packed into a packed bed column type enzyme reactor (with insulation jacket), and then a metering pump is used to continuously extract the reaction substrate from the reaction vessel and inject it into the enzyme reactor to immobilize the enzyme The transesterification reaction occurs under the catalysis of, and the reaction product flows out of the enzyme reactor and directly enters the container with the substrate, in this way the continuous cycle reaction is carried out. Adjust the flow rate to about 3L/hr to keep the oil in the enzyme reactor for 6 hours and then stop the reaction. The reaction product undergoes molecular distillation to further separate the ethyl ester, and the heavy fraction is then decolorized and deodorized in a conventional manner to obtain a grease composition 5.

[0027] After mixing composition 3 and a certain amount of glycerol (fatty acid group: glycerol=2, mol), add commercially available immobilized 1,3-specific lipase (Lipozyme RMIM, Novozymes A/S, or other commercial immobilized Lipase), while keeping the internal pressure of the system at 5mmHg absolute pressure, the mixture was allowed to react at 60°C under stirring until the free fatty acid content dropped to 10%, then the reaction was stopped, the immobilized enzyme particles were removed by filtration, and the remaining product was further subjected to molecular distillation After separation, followed by decolorization and deodorization in a conventional manner, an oil and fat composition 6 mainly composed of diglycerides was obtained.

[0028] The oil and fat composition 5 and the oil and fat composition 6 were uniformly mixed in a volume ratio of 10:90 to obtain a fat and oil composition 7.

[0029] Example 3

[0030] The oil and fat composition 1 and the oil and fat composition 2 were uniformly mixed in a volume ratio of 15:85 to obtain a fat and oil composition 8.