Organic-inorganic hybrid nanoflower and preparation method thereof
A nanoflower, organic technology, applied in biochemical equipment and methods, immobilized on or in inorganic carriers, glycosylases, etc., can solve the problems of reduced catalytic performance of biological enzymes, hindering the wide application of biocatalytic systems, etc. achieve high catalytic performance
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[0043] The present invention provides a method for preparing organic-inorganic hybrid nanoflowers described in the above scheme, comprising the following steps:
[0044](1) Mix the rare earth nitrate aqueous solution with biological enzymes to obtain a mixed solution; the rare earth ions in the rare earth nitrate are La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm One or more of , Yb and Y;
[0045] (2) After adding ammonium nitrate and ammonia water to the mixed liquid in sequence, aging is carried out to obtain an aged liquid;
[0046] (3) The aging liquid is centrifuged, washed and dried in sequence to obtain the organic-inorganic hybrid nanoflowers.
[0047] The invention mixes the rare earth nitrate aqueous solution and the biological enzyme to obtain the mixed solution. In the present invention, the aqueous solution of rare earth nitrates is preferably obtained by dissolving rare earth nitrates in water. In the present invention, the rare earth nitrate is preferably Ln...
Embodiment 1
[0056] (1) Set Y(NO 3 ) 3 •6H 2 O is dissolved in deionized water and is prepared into a yttrium nitrate solution with a total concentration of rare earth ions of 0.5mol / L;
[0057] (2) get the standby yttrium nitrate solution that quantitative step (1) makes, add horseradish peroxidase to yttrium nitrate solution under 15 ℃, the concentration of horseradish peroxidase in yttrium nitrate solution is 2.5mg / mL, stir well;
[0058] (3) the mixed solution of step (2) is added ammonium nitrate, and the concentration of ammonium nitrate in mixed solution is 1.5mol / L, stirs;
[0059] (4) Add the mixed solution of step (3) dropwise to the ammonia solution, and adjust the pH to 6;
[0060] (5) aging the mixed solution of step (4) for 24 h;
[0061] (6) After centrifuging the aged mixture, wash it with deionized water and absolute ethanol, and dry it in a blast drying oven at 30°C for 48 hours to obtain the rare earth layered compound as the inorganic carrier. Horseradish peroxid...
Embodiment 2
[0066] (1) Dy(NO 3 ) 3 •6H 2 O is dissolved in deionized water to prepare a dysprosium nitrate solution with a total concentration of rare earth ions of 0.1mol / L;
[0067] (2) Get the standby dysprosium nitrate solution prepared by the quantitative step (1), add horseradish peroxidase to the dysprosium nitrate solution at 25°C, the concentration of horseradish peroxidase in the dysprosium nitrate solution is 0.05mg / mL, stir well;
[0068] (3) the mixed solution of step (2) is added ammonium nitrate, and the concentration of ammonium nitrate in mixed solution is 0.5mol / L, stirs;
[0069] (4) Add the mixed solution of step (3) dropwise to the ammonia solution, and adjust the pH to 7.6;
[0070] (5) aging the mixed solution of step (4) for 72 h;
[0071] (6) After centrifuging the aged mixture, wash it with deionized water and absolute ethanol, and dry it in a blast drying oven at 45°C for 24 hours to obtain the rare earth layered compound as the inorganic carrier.
[0072...
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