Organic-inorganic hybrid nanoflower and preparation method thereof

A nanoflower, organic technology, applied in biochemical equipment and methods, immobilized on or in inorganic carriers, glycosylases, etc., can solve the problems of reduced catalytic performance of biological enzymes, hindering the wide application of biocatalytic systems, etc. achieve high catalytic performance

Active Publication Date: 2020-01-10
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
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Problems solved by technology

However, because the synthesis of most immobilized enzymes is carried out under harsh conditions, such as high temperature and pressure or the use of toxic organic so

Method used

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  • Organic-inorganic hybrid nanoflower and preparation method thereof
  • Organic-inorganic hybrid nanoflower and preparation method thereof
  • Organic-inorganic hybrid nanoflower and preparation method thereof

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[0043] The present invention provides a method for preparing organic-inorganic hybrid nanoflowers described in the above scheme, comprising the following steps:

[0044](1) Mix the rare earth nitrate aqueous solution with biological enzymes to obtain a mixed solution; the rare earth ions in the rare earth nitrate are La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm One or more of , Yb and Y;

[0045] (2) After adding ammonium nitrate and ammonia water to the mixed liquid in sequence, aging is carried out to obtain an aged liquid;

[0046] (3) The aging liquid is centrifuged, washed and dried in sequence to obtain the organic-inorganic hybrid nanoflowers.

[0047] The invention mixes the rare earth nitrate aqueous solution and the biological enzyme to obtain the mixed solution. In the present invention, the aqueous solution of rare earth nitrates is preferably obtained by dissolving rare earth nitrates in water. In the present invention, the rare earth nitrate is preferably Ln...

Embodiment 1

[0056] (1) Set Y(NO 3 ) 3 •6H 2 O is dissolved in deionized water and is prepared into a yttrium nitrate solution with a total concentration of rare earth ions of 0.5mol / L;

[0057] (2) get the standby yttrium nitrate solution that quantitative step (1) makes, add horseradish peroxidase to yttrium nitrate solution under 15 ℃, the concentration of horseradish peroxidase in yttrium nitrate solution is 2.5mg / mL, stir well;

[0058] (3) the mixed solution of step (2) is added ammonium nitrate, and the concentration of ammonium nitrate in mixed solution is 1.5mol / L, stirs;

[0059] (4) Add the mixed solution of step (3) dropwise to the ammonia solution, and adjust the pH to 6;

[0060] (5) aging the mixed solution of step (4) for 24 h;

[0061] (6) After centrifuging the aged mixture, wash it with deionized water and absolute ethanol, and dry it in a blast drying oven at 30°C for 48 hours to obtain the rare earth layered compound as the inorganic carrier. Horseradish peroxid...

Embodiment 2

[0066] (1) Dy(NO 3 ) 3 •6H 2 O is dissolved in deionized water to prepare a dysprosium nitrate solution with a total concentration of rare earth ions of 0.1mol / L;

[0067] (2) Get the standby dysprosium nitrate solution prepared by the quantitative step (1), add horseradish peroxidase to the dysprosium nitrate solution at 25°C, the concentration of horseradish peroxidase in the dysprosium nitrate solution is 0.05mg / mL, stir well;

[0068] (3) the mixed solution of step (2) is added ammonium nitrate, and the concentration of ammonium nitrate in mixed solution is 0.5mol / L, stirs;

[0069] (4) Add the mixed solution of step (3) dropwise to the ammonia solution, and adjust the pH to 7.6;

[0070] (5) aging the mixed solution of step (4) for 72 h;

[0071] (6) After centrifuging the aged mixture, wash it with deionized water and absolute ethanol, and dry it in a blast drying oven at 45°C for 24 hours to obtain the rare earth layered compound as the inorganic carrier.

[0072...

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Abstract

The invention provides an organic-inorganic hybrid nanoflower and a preparation method thereof, which relate to the technical field of enzyme immobilization. The organic-inorganic hybrid nanoflower provided by the invention is a flower-like immobilized enzyme formed by self-assembly compounding by taking a rare earth layered compound as an inorganic carrier and taking a biological enzyme as an organic component; wherein the rare earth layered compound is Ln2 (OH) 5NO3.nH2O, Ln is one or more of La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Y, and n is 1.1-2.5; wherein the biological enzyme is one or more of alpha-amylase, horse radish peroxidase and laccase. According to the invention, the rare earth layered compound is used as an inorganic carrier to load the biological enzyme and form the flower-like immobilized enzyme for the first time, and compared with free enzyme, the obtained immobilized enzyme has better stability and catalytic performance; the preparation method is mild in condition, simple in process and short in consumed time.

Description

technical field [0001] The invention relates to the technical field of enzyme immobilization, in particular to an organic-inorganic hybrid nanoflower and a preparation method thereof. Background technique [0002] Biological enzymes are an extremely important class of biocatalysts with high specificity and high catalytic performance. However, the industrial application of enzymes is still challenged due to the limitations of enzymes themselves or related process parameters, such as poor stability, difficult recovery, and high production costs. [0003] Enzyme immobilization is a technique that can effectively overcome these limitations. Compared with free enzymes, immobilized enzymes have several advantages: increased stability of free enzymes, improved enzyme reusability, and easier product isolation. However, because the synthesis of most immobilized enzymes is carried out under harsh conditions, such as high temperature and pressure or the use of toxic organic solvents,...

Claims

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Application Information

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IPC IPC(8): C12N11/14
CPCC12N11/14C12N9/0065C12N9/2414C12N9/0061C12Y110/03002C12Y302/01001C12Y111/01007Y02A50/20C01F17/276C01P2002/82C01P2004/03
Inventor 武晓鹂任珂王孟武贺泽文彭少玲宁继禅刘远立
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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