The invention provides an organic-inorganic hybrid nanoflower and a preparation method thereof, which relate to the technical field of enzyme immobilization. The organic-inorganic hybrid nanoflower provided by the invention is a flower-like immobilized enzyme formed by self-assembly compounding by taking a rare earth layered compound as an inorganic carrier and taking a biological enzyme as an organic component; wherein the rare earth layered compound is Ln2 (OH) 5NO3.nH2O, Ln is one or more of La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Y, and n is 1.1-2.5; wherein the biological enzyme is one or more of alpha-amylase, horse radish peroxidase and laccase. According to the invention, the rare earth layered compound is used as an inorganic carrier to load the biological enzyme and form the flower-like immobilized enzyme for the first time, and compared with free enzyme, the obtained immobilized enzyme has better stability and catalytic performance; the preparation method is mild in condition, simple in process and short in consumed time.