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A kind of fluorescence detection agent for specifically detecting arginine and preparation method of detection test paper

A technology for fluorescence detection and detection test paper, which is applied in the field of amino acid detection and can solve problems such as high cost and complexity

Active Publication Date: 2021-06-22
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although many methods for determining arginine have been reported at present, such as high performance liquid chromatography (HPLC), gas chromatography (GC) and capillary electrophoresis, most of these methods require special experimental equipment and complicated procedures, and the cost is relatively high

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  • A kind of fluorescence detection agent for specifically detecting arginine and preparation method of detection test paper
  • A kind of fluorescence detection agent for specifically detecting arginine and preparation method of detection test paper
  • A kind of fluorescence detection agent for specifically detecting arginine and preparation method of detection test paper

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Embodiment 1

[0021] Embodiment 1: Fluorescent detection agent for specific detection of arginine

[0022] (1) Preparation of DBAB: 4-(diethylamino)benzaldehyde (2.00g, 11.28mmol) was dissolved in dehydrated ethanol (130ml); 2-aminobenzoic acid (1.55g, 17.06mmol) was dissolved in N , in N-dimethylformamide (DMF), the concentration of 2-aminobenzoic acid is 20mmol / mL; then the N, N-dimethylformamide solution of 2-aminobenzoic acid and two drops of acetic acid (mass concentration 98%) was added in the dehydrated ethanol solution of 4-(diethylamino) benzaldehyde to form a mixture; The mixture was stirred at 50° C. for 5 hours, then cooled to room temperature, filtered, and finally washed solid with ethanol and hexane The product is (E)-2-((4-(diethylamino)benzylidene)amino)benzoic acid DBAB, a fluorescent detection agent for specifically detecting arginine.

[0023] (2) Detection of arginine: DBAB was dissolved in DMF at a concentration of 1.7 mM, and the solution was diluted to 0.169 mM with...

Embodiment 2

[0025] Embodiment 2: the detection test paper of specific detection arginine

[0026] (1) Preparation of test paper: pure white filter paper was cut into uniform strips and immersed in THF (tetrahydrofuran) solution (0.169 mM) of DBAB. After soaking for 5 minutes, remove and place in a fume hood to dry at room temperature.

[0027] (2) Application of test paper: drop the same amount of deionized water and 20 kinds of amino acids on the test paper, and observe the colors under ambient light and 365nm ultraviolet light respectively.

[0028] The test of the above-mentioned fluorescent detection agent for specifically detecting arginine and the detection test paper shows that the detection agent realizes faster detection and shortens the response time to 2s. The practical application potential of portability, low cost and fast response can be realized by applying it to test strips.

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Abstract

The invention discloses a fluorescent detection agent for specifically detecting arginine and a preparation method of a detection test paper. The detection reagent ((E)-2-((4-(diethylamino)benzylidene)amino)benzoic acid, DBAB) is a one-step synthesis of 4-(diethylamino)benzaldehyde and 2-aminobenzoic acid. Formula synthesis; then soak the white filter paper in the DBAB solution to prepare the detection test paper. In solution, DBAB showed specific recognition for arginine. In the colorimetric experiment, the mixed solution of DBAB‑Arg showed an obvious color change from yellow to colorless; in the fluorescence experiment, the mixed solution of DBAB‑Arg showed an obvious fluorescence change from cyan to purple. The same phenomenon also exists in the test paper experiment. According to the ultraviolet absorption spectrum and the fluorescence spectrum, DBAB is a multi-channel sensor with double absorption signals and double emission signals for arginine. The detection method has high selectivity and transient response.

Description

technical field [0001] The invention belongs to the technical field of amino acid detection, and in particular relates to a fluorescent detection agent for specifically detecting arginine and a preparation method of a detection test paper. Background technique [0002] Of the 20 common amino acids in the human body, arginine has attracted attention for its important effects on the chemistry of the human brain, including dilation of blood vessels, immune response and neurotransmission. Arginine (Arg), also known as protein amino acid, is the most basic amino acid. Compared with other 20 kinds of amino acids, its isoelectric point (pI) is the highest, which is 10.76. Arginine plays an important role in various biological functions such as cell division, wound healing, protein production, immune response, etc. In addition, arginine participates in the ornithine cycle in the human body, promotes the formation of urea, converts the ammonia produced in the human body into non-tox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N21/78G01N21/64
CPCG01N21/6428G01N21/78G01N33/68
Inventor 黄潇楠路昭然朱晓雨韩天宇
Owner CAPITAL NORMAL UNIVERSITY