Application of Methyl 2-(methylamino)benzoate
A technology of methyl benzoate and methylamino, which is applied in the direction of medical preparations containing active ingredients, antibacterial drugs, organic active ingredients, etc., to achieve the effects of easy access, small effective dose, and small potential side effects
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Embodiment 1
[0036] Example 1: Effect of 2-(methylamino)methyl benzoate on the growth of Pseudomonas aeruginosa
[0037] To the Pseudomonas aeruginosa that adds the 2-(methylamino) methyl benzoate effect 48h of different concentrations, the growth curve drawn is as follows figure 1 As shown, different concentrations of PCR-M had no significant inhibition on the growth of Pseudomonas aeruginosa.
Embodiment 2
[0038] Example 2: Effect of methyl 2-(methylamino)benzoate on pyocyanin, elastase, rhamnolipid and biofilm of Pseudomonas aeruginosa
[0039] The inhibitory effect of PCR-M on Pseudomonas aeruginosa was investigated according to the experimental methods of pyocyanin, elastase, rhamnolipid and biofilm, and the results are shown in Table 1. The experimental results showed that the inhibition rates of 2.5mg / mL PCR-M to Pseudomonas aeruginosa pyocyanin, elastase, rhamnolipid and biofilm were 86.4%, 20.5%, 40.5% and 46.8%, respectively. One-way analysis of variance showed that PCR-M had significant inhibitory effect on virulence factors (including pyocyanin, elastase, rhamnolipid) and biofilm formation of Pseudomonas aeruginosa (P<0.05).
[0040] Table 1 is the inhibition of pyocyanin, elastase, rhamnolipid and biofilm of Pseudomonas aeruginosa by 2-(methylamino) methyl benzoate
[0041]
[0042] Among them, different letters * and ** in the table indicate that the differences ...
Embodiment 3
[0043] Embodiment 3: 2-(Methylamino) benzoic acid methyl ester affects the real-time fluorescence quantitative PCR of the genes related to virulence factors and biofilm formation in Pseudomonas aeruginosa quorum sensing system
[0044] Real-time quantitative PCR experiments were used to detect the regulation of PCR-M on the transcription level of genes related to the quorum sensing system of Pseudomonas aeruginosa. The genes with significant changes were as follows: figure 2 shown.
[0045] The experimental results show that the relative expression levels of genes lasA, pqsA, phzM, mvfR, pelF, lasR, chiC, lasI, lasB, rhlI, rhlR, pslA, rpoS and other genes related to virulence factors or biofilm formation during the growth of Pseudomonas aeruginosa Both were down-regulated, and the difference was extremely significant (P<0.001); this indicates that PCR-M has a significant regulatory effect on the transcription level of the quorum sensing system of Pseudomonas aeruginosa, there...
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