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Tuna white meat ACE inhibitory peptide and preparation method thereof

A technology of tuna and inhibitory peptides is applied in the field of tuna white meat ACE inhibitory peptide and its preparation, which can solve the problems of high cost of blood pressure-lowering peptide, unsuitable for industrial production, low blood pressure lowering activity and the like, and achieves good inhibitory activity, low preparation cost, Efficient effect

Active Publication Date: 2020-01-24
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problems of high cost, low efficiency, inapplicability to industrial production, and low antihypertensive activity of existing antihypertensive peptides, the present invention proposes a tuna white meat ACE inhibitory peptide and a preparation method thereof

Method used

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  • Tuna white meat ACE inhibitory peptide and preparation method thereof
  • Tuna white meat ACE inhibitory peptide and preparation method thereof
  • Tuna white meat ACE inhibitory peptide and preparation method thereof

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Embodiment 1-15

[0041] Embodiment 1-15: a preparation method of tuna white meat ACE inhibitory peptide, comprising the following steps:

[0042] 1) Thaw the white meat of the tuna, dry it to remove the water, then add ethyl acetate to the tuna, immerse it to degrease, rotate it, dry it, and pulverize it for later use;

[0043] 2) adding distilled water to the pretreated white meat powder, adjusting the pH value to 8.5-10.5, adding 1-3 wt% alkaline protease, and enzymatically hydrolyzing at 45-65°C for 3 hours to obtain an enzymolyzed solution;

[0044] 3) Heat the enzymatic hydrolysis solution in a water bath at 100°C for 10 minutes to inactivate the enzyme, then centrifuge at 4000r for 20 minutes, take the supernatant to obtain the enzymatic hydrolysis solution inactivating the enzyme, then freeze-dry to obtain the polypeptide powder, and measure the ACE inhibitory activity;

[0045]4) Use a 3.5KDa ultrafiltration membrane to carry out ultrafiltration classification on the enzymatic hydrolyz...

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Abstract

The invention discloses a tuna white meat ACE inhibitory peptide and a preparation method thereof, which relate to the field of ACE inhibitory peptide preparation, a peptide fragment amino acid sequence of the tuna white meat ACE inhibitory peptide is Ser-Pro or Val-Asp-Arg-Tyr-Phe, and the preparation method comprises the following preparation steps: 1) thawing tuna white meat, drying to remove water, degreasing and drying; 2) adding distilled water into the pretreated white meat powder, adjusting the pH value, and adding protease for enzymolysis to obtain an enzymatic hydrolysate; 3) carrying out enzyme deactivation treatment on the enzymatic hydrolysate, centrifuging, taking a supernate to obtain an enzyme-deactivated enzymatic hydrolysate, freeze-drying to obtain polypeptide powder, and measuring ACE inhibitory activity; and 4) performing ultrafiltration, column chromatography, high performance liquid chromatography purification and amino acid sequencing on the enzyme-deactivated enzymatic hydrolysate to obtain the tuna white meat ACE inhibitory peptide. The tuna white meat ACE inhibitory peptide prepared by the method is good in inhibitory activity, low in preparation cost, high in efficiency and suitable for industrial production.

Description

technical field [0001] The invention relates to the field of preparation of ACE inhibitory peptides, in particular to an ACE inhibitory peptide of tuna white meat and a preparation method thereof. Background technique [0002] Hypertension is a systemic disease characterized by elevated arterial vascular systolic or (and) diastolic blood pressure, and causes various complications such as stroke, myocardial infarction, heart failure, dementia, liver and kidney failure, and blindness. It is an important public health problem in our country and even in the world. Human blood pressure regulation is related to various nervous and humoral systems, such as sympathetic nervous system, renin-angiotensin system (Renin-Angiotensin System, RAS), kallikrein-kinin system (Kallikrein-Kinin System, KKS) , kidney and body fluid balance system, and nitric oxide-endothelin system. Among them, the renin-angiotensin system and the kallikrein-kinin system play an important role in the regulatio...

Claims

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Application Information

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IPC IPC(8): C07K5/062C07K7/06A61P9/12C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16
CPCA61P9/12C07K5/06069C07K7/06C12P21/06
Inventor 赵玉勤
Owner ZHEJIANG OCEAN UNIV
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