Neisseria gonorrhoeae agar plate and preparation method thereof

Abstract

The invention discloses a Neisseria gonorrhoeae agar plate and a preparation method thereof, and relates to the technical field of culture medium. The Neisseria gonorrhoeae agar plate is more comprehensive in nutrition and capable of effectively promoting the growth of Neisseria gonorrhoeae and improving the separation rate of Neisseria gonorrhoeae. According to the preparation method, corn starchis separated to be subjected to high temperature dissolution since the corn starch is not easy to dissolve, thus ensuring the dissolving efficiency and avoiding the nutrient damage to other components caused by high temperature. Meanwhile, after high temperature sterilization of the culture medium, the mixed solution is subjected to heating hematolysis, so that the V factor and the X factor in red blood cells are released. During heating, a short time heating is carried out at a higher temperature to promote the rapid rupture of cell walls, and then a continuous heating is carried out at a lower temperature to ensure the full release of substances in the red blood cells and avoid the damage to other nutrient substances by high temperature. The preparation method is simple to operate, lowin requirements on equipment, capable of effectively improving product quality and suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of medium, in particular to a gonorrhoeae agar plate and a preparation method thereof. Background technique [0002] Pathogenic bacteria or opportunistic pathogenic bacteria exist in the infected site, leading to common clinical infections, such as respiratory tract infection, reproductive tract infection, intestinal infection, etc. In order to accurately diagnose the type of bacteria in the infected site, patients are often used clinically Samples are collected from the site for culture, and clinical examiners select suspicious strains for identification based on clinical symptoms, so as to determine the type of infected bacteria in the patient's site and provide a basis for clinical treatment. Diagnosis and culture of infected bacterial types in patient parts are currently the only feasible methods in clinical diagnosis. Other methods, such as immune antigen detection and nucleic acid detection, are not su...

AI Technical Summary

Problems solved by technology

[0003] The existing Neisseria gonorrhoeae agar medium is not very comprehensive and uniform in nutrient composition, and the effect on the cultivation of Neisseria gonorrhoeae is not good

Moreover, the existing method for preparing Neisseria gonorrhoeae agar is more suitable for small-scale self-production and self-use. During large-scale production, there will be agglomeration, overheating, etc., which will affect product quality.