Widely-applicable plant tissue culture medium and 1/2 culture medium

Abstract

The invention discloses a widely-applicable plant tissue culture medium and a 1 / 2 culture medium. Each 1000 mL of the culture medium comprises 2662 mg of KNO3, 264 mg of (NH4)2SO4, 288 mg of NH4H2PO4,370 mg of MgSO4.7H2O, 332 mg of CaCI2, 15 mg of MnSO4.H2O, 4 mg of ZnSO4.7H2O, 3 mg of H3BO3, 0.02 mg of CoCl2.6H2O, 0.04 mg of CuSO4.5H2O, 0.1 mg of Na2MoO4.2H2O, 37 mg of NaFeEDTA, 100 mg of inositol, 2 mg of nicotinic acid, 7 mg of thiamine hydrochloride, 1 mg of pyridoxine hydrochloride, and 2 mg of glycine. The content of macro elements is halved to obtain the 1 / 2 CS tissue culture medium. The plant tissue culture medium disclosed by the invention can be widely applied to various plant tissue cultures, the culture effect is equal to or better than that of an MS culture medium, the explosive reagent NH4NO3 contained in the existing culture medium is removed on the premise that new explosive components are not introduced, and the purchase, storage and management of the culture medium are facilitated; moreover, pH stability of a liquid culture medium prepared with the plant tissue culture medium is good.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a plant tissue culture medium. Background technique [0002] Based on the design of Murashige and Skoog in 1962, MS culture is the most widely used medium in plant tissue culture. According to the "Regulations on the Safety Management of Hazardous Chemicals" (Order No. 591 of the State Council), the "Regulations on the Safety Management of Civil Explosives" and the "List of Predisposing Hazardous Chemicals" (2017 edition), the main components of MS culture medium are potassium nitrate, Ammonium nitrate is an explosive control reagent. With the stricter national supervision, the purchase, storage and use of all explosive preparation reagents have become more and more difficult, especially ammonium nitrate, which has both nitrate nitrogen and ammonium nitrogen. Its pure product is banned from market circulation, which brings great difficulty to plant tissue culture. ...

AI Technical Summary

Problems solved by technology

According to the "Regulations on the Safety Management of Hazardous Chemicals" (Decree No. 591 of the State Council), the "Regulations on the Safety Management of Civilian Explosives" and the "List of Hazardous Chemicals Predisposing to Explosives" (2017 edition), the main components of MS medium are potassium nitrate, Ammonium nitrate is a controlled reagent for explosive precursors. As the national supervision becomes stricter, it becomes more and more difficult to purchase, store and use all explosive precursor reagents, especially ammonium nitrate with both nitrate nitrogen and ammonium nitrogen. Its pure product is banned from market circulation, which brings great difficulties to plant tissue culture

B5 culture is based on the design by Gamborg et al. for the cultivation of soybean root cells in 1968, and N6 culture is based on the design by Zhu Zhiqing et al. in 1974 for the cultivation of anthers of cereal crops such as rice. Both are derived from MS medium and are used in some plant tissues. It is widely used in culture research. Although the B5 and N6 mediums do not contain other explosive components except potassium nitrate, the contents of calcium ions and magnesium ions in both of them are greatly reduced, and the ammonium ions in the B5 medium are greatly reduced, and the N6 Potassium ions in the medium increase significantly, and large fluctuations in the concentration of these ions are detrimental to the tissue culture of some plants

CN102224802A Strawberry Proliferation Medium discloses a medium without ammonium nitrate, but its content of potassium nitrate and ammonium sulfate is too high, so it is only suitable for strawberry tissue culture; CN105104208A A kind of strawberry tissue culture medium and its preparation method discloses a medium without nitric acid Ammonium medium, which has achieved good results in strawberry tissue culture, but the content of urea and ammonium sulfate introduced in it is too high, which is unfavorable to the growth of various plants; CN108812313A is a basic medium for plant tissue culture and CN108812329A is a A medium for plant tissue culture without NH4NO3 respectively discloses a nitric acid-free medium, which obtains a good culture effect, but the newly introduced calcium nitrate and magnesium nitrate are both explosive reagents; CN109717076A A kind of plant tissue culture The special medium discloses a medium without ammonium nitrate, which can improve the success rate of plant tissue culture, but introduces the explosive reagent sodium nitrate, and it is only suitable for plant in vitro culture, and has no versatility

In addition, existing plant tissue culture media, including MS, B5, N6 and other disclosed plant tissue culture media, have large pH fluctuations when they are used to prepare liquid culture media, and all need to be adjusted when they are used for plant hydroponics pH, the process is tedious and time consuming

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