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A kind of wide-adapted plant tissue culture medium and 1/2 culture medium

A plant tissue and culture medium technology, applied in the field of plant tissue culture, can solve the problems of cumbersome process, unfavorable plant growth, time-consuming, etc., and achieve the effect of promoting plant germination, simplifying the preparation process, and eliminating potential safety hazards

Active Publication Date: 2022-03-08
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the "Regulations on the Safety Management of Hazardous Chemicals" (Decree No. 591 of the State Council), the "Regulations on the Safety Management of Civilian Explosives" and the "List of Hazardous Chemicals Predisposing to Explosives" (2017 edition), the main components of MS medium are potassium nitrate, Ammonium nitrate is a controlled reagent for explosive precursors. As the national supervision becomes stricter, it becomes more and more difficult to purchase, store and use all explosive precursor reagents, especially ammonium nitrate with both nitrate nitrogen and ammonium nitrogen. Its pure product is banned from market circulation, which brings great difficulties to plant tissue culture
B5 culture is based on the design by Gamborg et al. for the cultivation of soybean root cells in 1968, and N6 culture is based on the design by Zhu Zhiqing et al. in 1974 for the cultivation of anthers of cereal crops such as rice. Both are derived from MS medium and are used in some plant tissues. It is widely used in culture research. Although the B5 and N6 mediums do not contain other explosive components except potassium nitrate, the contents of calcium ions and magnesium ions in both of them are greatly reduced, and the ammonium ions in the B5 medium are greatly reduced, and the N6 Potassium ions in the medium increase significantly, and large fluctuations in the concentration of these ions are detrimental to the tissue culture of some plants
CN102224802A Strawberry Proliferation Medium discloses a medium without ammonium nitrate, but its content of potassium nitrate and ammonium sulfate is too high, so it is only suitable for strawberry tissue culture; CN105104208A A kind of strawberry tissue culture medium and its preparation method discloses a medium without nitric acid Ammonium medium, which has achieved good results in strawberry tissue culture, but the content of urea and ammonium sulfate introduced in it is too high, which is unfavorable to the growth of various plants; CN108812313A is a basic medium for plant tissue culture and CN108812329A is a A medium for plant tissue culture without NH4NO3 respectively discloses a nitric acid-free medium, which obtains a good culture effect, but the newly introduced calcium nitrate and magnesium nitrate are both explosive reagents; CN109717076A A kind of plant tissue culture The special medium discloses a medium without ammonium nitrate, which can improve the success rate of plant tissue culture, but introduces the explosive reagent sodium nitrate, and it is only suitable for plant in vitro culture, and has no versatility
In addition, existing plant tissue culture media, including MS, B5, N6 and other disclosed plant tissue culture media, have large pH fluctuations when they are used to prepare liquid culture media, and all need to be adjusted when they are used for plant hydroponics pH, the process is tedious and time consuming

Method used

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  • A kind of wide-adapted plant tissue culture medium and 1/2 culture medium
  • A kind of wide-adapted plant tissue culture medium and 1/2 culture medium
  • A kind of wide-adapted plant tissue culture medium and 1/2 culture medium

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment one, the extensive adaptability plant tissue culture medium of this embodiment, it contains in every 1000mL culture medium: KNO 3 2662mg, (NH 4 ) 2 SO 4 264mg, NH 4 h 2 PO 4 288mg, MgSO 4 ·7H 2 O 370mg, CaCl 2 332mg, MnSO 4 ·H 2 O15mg, ZnSO 4 ·7H 2 O 4mg, H 3 BO 3 3mg, CoCl 2 ·6H 2 O 0.02mg, CuSO 4 ·5H 2 O 0.04mg, Na 2 MoO 4 2H 2 O 0.1mg, NaFeEDTA 37mg, inositol 100mg, niacin 2mg, thiamine hydrochloride 7mg, pyridoxine hydrochloride 1mg, glycine 2mg. The broad-adapted plant tissue culture medium in this example is named CS basic medium.

Embodiment 2

[0025] Embodiment two, present embodiment wide adaptability plant tissue 1 / 2 culture medium, it contains in every 1000mL culture medium: KNO 3 1331mg, (NH 4 ) 2 SO 4 132mg, NH 4 h 2 PO 4 144mg, MgSO 4 ·7H 2 O 185mg, CaCl 2 166mg, MnSO 4 ·H 2 O15mg, ZnSO 4 ·7H 2 O 4mg, H 3 BO 3 3mg, CoCl 2 ·6H 2 O 0.02mg, CuSO 4 ·5H 2 O 0.04mg, Na 2 MoO 4 2H 2 O 0.1mg, NaFeEDTA 37mg, inositol 100mg, niacin 2mg, thiamine hydrochloride 7mg, pyridoxine hydrochloride 1mg, glycine 2mg. The wide-adapted plant tissue 1 / 2 medium in this example is named 1 / 2CS basic medium.

[0026] The CS basal medium and 1 / 2CS basal medium in the above-mentioned embodiment are compared with the components of the existing MS basal medium and 1 / 2MS basal medium as shown in Table 1:

[0027] Table 1 MS, CS, 1 / 2MS, 1 / 2CS basic medium composition list

[0028]

[0029]

[0030] The CS basal medium and 1 / 2CS basal medium in the above examples, if applied to the production of solid medium in ...

example 1

[0031] Cultivation Example 1: Culture of Columbia-type Arabidopsis sterilized seedlings

[0032] (1) Seed preservation and disinfection methods:

[0033] a. Preservation method of Arabidopsis thaliana seeds: After the Arabidopsis thaliana seeds of Columbia (Col, Columbia) mature, collect the seeds in a 1.5mL centrifuge tube, add 3-8 pieces of color-changing silica gel desiccant, seal with parafilm and place in 4 ℃ long-term storage;

[0034] b. Sterilization method for Arabidopsis seeds: Take out the seeds that have been dried and preserved at low temperature, put an appropriate amount of seeds in a sterile 1.5mL centrifuge tube in a sterile environment, add an appropriate volume of sterile water, and turn the centrifuge tube upside down for several Centrifuge with a low-speed centrifuge for 10 seconds after the first time, pour off the supernatant carefully, and repeat 3 times; add 75% alcohol, turn the centrifuge tube upside down several times, and centrifuge for 10 seconds...

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Abstract

The invention discloses a wide-adaptability plant tissue culture medium and 1 / 2 culture medium, each 1000mL culture medium contains: KNO 3 2662mg, (NH 4 ) 2 SO 4 264mg, NH 4 h 2 PO 4 288mg, MgSO 4 ·7H 2 O 370mg, CaCl 2 332mg, MnSO 4 ·H 2 O 15mg, ZnSO 4 ·7H 2 O 4mg, H 3 BO 3 3mg, CoCl 2 ·6H 2 O 0.02mg, CuSO 4 ·5H 2 O 0.04mg, Na 2 MoO 4 2H 2 O 0.1mg, NaFeEDTA 37mg, inositol 100mg, niacin 2mg, thiamine hydrochloride 7mg, pyridoxine hydrochloride 1mg, glycine 2mg; halve the content of macroelements to obtain 1 / 2CS tissue culture medium. The plant tissue culture medium of the present invention can be widely used in various plant tissue cultures, the culture effect is equivalent to or better than MS medium, and it removes the existing There are explosive reagents NH contained in the culture medium 4 NO 3 , which is convenient for the purchase, storage and management of the medium; and the pH stability of the liquid medium prepared with it is good.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a plant tissue culture medium. Background technique [0002] MS culture is based on the design by Murashige and Skoog in 1962 and is currently the most widely used medium in plant tissue culture. According to the "Regulations on the Safety Management of Hazardous Chemicals" (Decree No. 591 of the State Council), the "Regulations on the Safety Management of Civilian Explosives" and the "List of Hazardous Chemicals Predisposing to Explosives" (2017 edition), the main components of MS medium are potassium nitrate, Ammonium nitrate is a controlled reagent for explosive precursors. As the national supervision becomes stricter, it becomes more and more difficult to purchase, store and use all explosive precursor reagents, especially ammonium nitrate with both nitrate nitrogen and ammonium nitrogen. Its pure product is prohibited from market circulation, which brings great...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 陈新龙尚丽娜何光华桑贤春邢亚迪杜丹张莹莹赵芳明凌英华涂丹
Owner SOUTHWEST UNIV
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