A kind of wide-adapted plant tissue culture medium and 1/2 culture medium
A plant tissue and culture medium technology, applied in the field of plant tissue culture, can solve the problems of cumbersome process, unfavorable plant growth, time-consuming, etc., and achieve the effect of promoting plant germination, simplifying the preparation process, and eliminating potential safety hazards
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Embodiment 1
[0024] Embodiment one, the extensive adaptability plant tissue culture medium of this embodiment, it contains in every 1000mL culture medium: KNO 3 2662mg, (NH 4 ) 2 SO 4 264mg, NH 4 h 2 PO 4 288mg, MgSO 4 ·7H 2 O 370mg, CaCl 2 332mg, MnSO 4 ·H 2 O15mg, ZnSO 4 ·7H 2 O 4mg, H 3 BO 3 3mg, CoCl 2 ·6H 2 O 0.02mg, CuSO 4 ·5H 2 O 0.04mg, Na 2 MoO 4 2H 2 O 0.1mg, NaFeEDTA 37mg, inositol 100mg, niacin 2mg, thiamine hydrochloride 7mg, pyridoxine hydrochloride 1mg, glycine 2mg. The broad-adapted plant tissue culture medium in this example is named CS basic medium.
Embodiment 2
[0025] Embodiment two, present embodiment wide adaptability plant tissue 1 / 2 culture medium, it contains in every 1000mL culture medium: KNO 3 1331mg, (NH 4 ) 2 SO 4 132mg, NH 4 h 2 PO 4 144mg, MgSO 4 ·7H 2 O 185mg, CaCl 2 166mg, MnSO 4 ·H 2 O15mg, ZnSO 4 ·7H 2 O 4mg, H 3 BO 3 3mg, CoCl 2 ·6H 2 O 0.02mg, CuSO 4 ·5H 2 O 0.04mg, Na 2 MoO 4 2H 2 O 0.1mg, NaFeEDTA 37mg, inositol 100mg, niacin 2mg, thiamine hydrochloride 7mg, pyridoxine hydrochloride 1mg, glycine 2mg. The wide-adapted plant tissue 1 / 2 medium in this example is named 1 / 2CS basic medium.
[0026] The CS basal medium and 1 / 2CS basal medium in the above-mentioned embodiment are compared with the components of the existing MS basal medium and 1 / 2MS basal medium as shown in Table 1:
[0027] Table 1 MS, CS, 1 / 2MS, 1 / 2CS basic medium composition list
[0028]
[0029]
[0030] The CS basal medium and 1 / 2CS basal medium in the above examples, if applied to the production of solid medium in ...
example 1
[0031] Cultivation Example 1: Culture of Columbia-type Arabidopsis sterilized seedlings
[0032] (1) Seed preservation and disinfection methods:
[0033] a. Preservation method of Arabidopsis thaliana seeds: After the Arabidopsis thaliana seeds of Columbia (Col, Columbia) mature, collect the seeds in a 1.5mL centrifuge tube, add 3-8 pieces of color-changing silica gel desiccant, seal with parafilm and place in 4 ℃ long-term storage;
[0034] b. Sterilization method for Arabidopsis seeds: Take out the seeds that have been dried and preserved at low temperature, put an appropriate amount of seeds in a sterile 1.5mL centrifuge tube in a sterile environment, add an appropriate volume of sterile water, and turn the centrifuge tube upside down for several Centrifuge with a low-speed centrifuge for 10 seconds after the first time, pour off the supernatant carefully, and repeat 3 times; add 75% alcohol, turn the centrifuge tube upside down several times, and centrifuge for 10 seconds...
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