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Extraction method of antarctic krill protein

An Antarctic krill and extraction method technology, applied in the field of protein extraction, can solve the problems of krill protein production decline, krill protein impurities, heavy fishy smell, etc., and achieve the effect of increasing the extraction amount and improving the removal effect

Pending Publication Date: 2020-02-07
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese Patent Publication No. CN103583789 discloses a method for ultrasonic-assisted extraction of protein isolated from Antarctic krill, which sequentially includes alkaline leaching, ultrasonic extraction, centrifugation, secondary ultrasonic extraction, acid precipitation, alcohol washing, and water washing; On the basis of protein, an ultrasonic-assisted method is used to optimize the extraction process of Antarctic krill protein isolate, but the obtained krill protein has more impurities and a heavier fishy smell; Chinese Patent Publication No. CN106798073 discloses a A method for extracting protein from krill. This method uses Antarctic shrimp as raw material, and uses the endogenous protease of Antarctic shrimp to autolyze in stages at different temperatures, so that the best effect of different endogenous proteases can be maximized. Separate the protein in Antarctic krill, use activated carbon to decolorize and deodorize the krill protein, remove bitter amino acids and fishy trimethylamine nitrogen, but the adsorption of activated carbon can also adsorb the protein after enzymatic hydrolysis of Antarctic krill, resulting in krill decrease in protein production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting Antarctic krill protein, comprising the following steps:

[0025] 1) Freeze-dry 5kg of Antarctic krill, pulverize and sieve, add distilled water at a material-to-liquid ratio of 1:10, mix and homogenize to obtain a slurry;

[0026] 2) adding sodium hydroxide solution with a mass concentration of 8% to the slurry to adjust the pH to 9.5, and then adding 10 g of protease for enzymolysis for 1.5 hours to obtain an enzymolysis solution;

[0027] 3) Ultrasonicate the enzymatic solution for 30 minutes at 200W power, then inactivate protease, and centrifuge at 8000r / min for 30 minutes to obtain the protein supernatant;

[0028] 4) adding a mass concentration of 10% hydrochloric acid solution to the protein supernatant to adjust the pH to 4, acid precipitation, and then adding sepiolite for adsorption treatment, the amount of sepiolite being 3wt% of the supernatant; then adding Adjust the pH to 11 with 8% sodium hydroxide solution, centrifuge at 8000r / m...

Embodiment 2

[0032] The preparation method of modified sepiolite comprises the following steps:

[0033] Add 5g of sepiolite to 100mL of 10% ethanol aqueous solution, stir and disperse evenly to obtain a suspension, add 0.5g of KH-550 silane coupling agent to the suspension, heat in a water bath to 45°C, stir for 1.5h, filter , and dry to obtain alkylated sepiolite; adding the alkylated sepiolite to a concentration of 1wt% polyethyleneimine aqueous solution, the mass volume ratio of alkylated sepiolite to polyethyleneimine aqueous solution is 2g / 50mL, Raise the temperature to 62°C and keep it warm for 4 hours to obtain the product.

[0034] A method for extracting Antarctic krill protein, comprising the following steps:

[0035] 1) Freeze-dry 5kg of Antarctic krill, pulverize and sieve, add distilled water at a material-to-liquid ratio of 1:10, mix and homogenize to obtain a slurry;

[0036] 2) adding sodium hydroxide solution with a mass concentration of 8% to the slurry to adjust the p...

Embodiment 3

[0042] The preparation method of modified sepiolite comprises the following steps:

[0043] Add 5g of sepiolite to 100mL of 10% ethanol aqueous solution, stir and disperse evenly to obtain a suspension, add 0.5g of KH-550 silane coupling agent to the suspension, heat to 50°C in a water bath, stir for 1.5h, filter , and dry to obtain alkylated sepiolite; the alkylated sepiolite is added to a concentration of 2.5wt% polyethyleneimine aqueous solution, and the mass volume ratio of alkylated sepiolite to polyethyleneimine aqueous solution is 2g / 50mL , heating up to 65°C, and keeping the temperature for 3h, that is.

[0044] A method for extracting Antarctic krill protein, comprising the following steps:

[0045] 1) Freeze-dry 5kg of Antarctic krill, pulverize and sieve, add distilled water at a material-to-liquid ratio of 1:10, mix and homogenize to obtain a slurry;

[0046]2) adding sodium hydroxide solution with a mass concentration of 10% to the slurry to adjust the pH to 10,...

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PUM

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Abstract

The invention relates to the technical field of protein extraction and discloses an extraction method of antarctic krill protein. The extraction method comprises the following steps: 1) freeze-dryingantarctic krill, performing crushing and sieving, adding the sieved antarctic krill into distilled water, and performing mixing and homogenization to form slurry, 2) adding a sodium hydroxide solutioninto the slurry to regulate pH to 9-10, and adding protease for enzymolysis to form an enzymolysis solution, 3) performing ultrasonic treatment on the enzymolysis solution, and performing protease inactivation and centrifugation to obtain a protein supernate, 4) adding a hydrochloric acid solution into the protein supernate for acid sediment, adding meerschaum for absorption treatment, adding thesodium hydroxide solution to regulate the pH to 10-13, performing centrifugation and taking supernate, and 5) adding the hydrochloric acid solution into the supernate for secondary acid sediment, andperforming filtration, separation, washing and freeze-drying to obtain the antarctic krill protein. The meerschaum is added for deodorization treatment on the krill protein in an antarctic krill protein extraction process, and does not absorb the krill protein, and an extraction rate of the krill protein is increased.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a method for extracting Antarctic krill protein. Background technique [0002] Antarctic krill is the most abundant and urgently developed fishery resource on the earth. Because of its high nutritional value, it can be used as a potential food source for humans. As a rich strategic resource, the development and utilization of Antarctic krill is an effective use of global resources and has important strategic significance to our country. As a polar marine organism with huge resources and high nutritional value, its protein content is high, and its amino acid composition meets the high-quality protein standards stipulated by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO). While providing sufficient nutrition, it reduces cholesterol. risk of ingestion. It is a high-quality protein with good potential for develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04A23L5/20
CPCA23J1/04A23L5/273
Inventor 欧阳小琨卢雨清杨立业范丽红
Owner ZHEJIANG OCEAN UNIV